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Method of detecting content of phenylethanolamine A and detection kit

A detection kit and technology of phenylethanolamine, which is applied in the research field of food safety supervision or food analysis, can solve the problems of lack of high sensitivity and high specificity, and achieve the effect of low test cost, high sensitivity and improved sensitivity

Active Publication Date: 2013-08-28
SUZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Yet so far, still lack the ELISA detection method of high sensitivity and high specificity phenylethanolamine A

Method used

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  • Method of detecting content of phenylethanolamine A and detection kit
  • Method of detecting content of phenylethanolamine A and detection kit
  • Method of detecting content of phenylethanolamine A and detection kit

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Embodiment 1

[0053] 1. Preparation of phenylethanolamine A modification

[0054] 1) phenylethanolamine A hapten (PA-NH 2 )Synthesis

[0055] Weigh PA and dissolve it in absolute ethanol, heat up to 80°C, add hydrazine hydrate (PA and hydrazine hydrate are added in equimolar amounts), palladium carbon is used as a catalyst, and reflux at 80°C for 1-10 hours under nitrogen protection (until the reaction is complete ). After the reaction, suction filtration was performed, and the filtrate was spin-dried to obtain a pale yellow crude product. Use dichloromethane / methanol / triethylamine to pass through the column according to the solvent ratio of (15:1:0.05) to obtain pure product, the product is abbreviated as PA-NH 2 , and its reaction formula is as follows:

[0056]

[0057] 2) phenylethanolamine A hapten (PA-NH 2 ) of the characterization:

[0058] 1 H-NMR spectrum: Bruker AMX-400 nuclear magnetic resonance instrument is used to test the nuclear magnetic spectrum of phenylethanola...

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Abstract

The invention discloses a method of detecting a content of phenylethanolamine A and a detection kit. According to the method, immunogens and coating antigens are prepared by synthesizing hapten derivatives of the phenylethanolamine A and crosslinking the derivatives with carrier proteins. Four kinds of polyclonal antibodies of rabbit anti-phenylethanolamine A through immunizing animals are obtained and all the four antibodies can be used for immunoassay of the phenylethanolamine A. The invention further provides an ELISA detection kit containing the antigens and / or antibodies. The ELISA method built by the invention is high in sensitivity and strong in specificity. Four kinds of samples: pig kidney, pig liver, pork and pig forage, are added with different amounts of the phenylethanolamine A for an addition and reclamation test, and the test shows that the detecting method provided by the invention is accurate, and has relatively good correlation with an LC-MS-MS method.

Description

technical field [0001] The invention belongs to the research field of food safety supervision or food analysis, and relates to an enzyme-linked immunosorbent assay method (ELISA) for detecting the content of phenylethanolamine A, and also relates to a related detection kit. Background technique [0002] β-adrenoceptor agonist (abbreviated as β-agonist) is a kind of phenylethanol derivatives whose structure and physiological function are similar to epinephrine and norepinephrine, because it can interact with most tissue cell membranes in animals. Named for binding to β-adrenergic receptors. β-agonists can significantly improve carcass lean percentage, weight gain and improve feed conversion ratio. Therefore, β-agonists are widely used illegally in livestock production, and the most commonly used ones are clenbuterol, salbutamol, ractopamine, etc. There have been many reports of poisoning caused by eating animal liver or lung tissue containing β-agonist residues at home and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C217/70C07C213/08G01N33/566C07K14/765C07K14/77C07K16/44
Inventor 邓安平曹碧云杨红
Owner SUZHOU UNIV
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