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Phenylethanolamine A magnetic molecular imprinted polymer microsphere as well as preparation method and application thereof

A magnetic molecular imprinting, phenylethanolamine technology, applied in the directions of alkali metal compounds, chemical instruments and methods, ion exchange, etc., can solve the problems of illegal use of feeding animals, making huge profits, etc., and achieve good inclusion complexing ability and chemical stability. properties, preventing agglomeration and oxidation

Inactive Publication Date: 2014-01-29
INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, driven by profit, there are still some lawbreakers who regard it as a substitute for "Clenbuterol" (Clenbuterol hydrochloride), illegally use it in feed or feeding to feed animals, and make huge profits from it

Method used

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  • Phenylethanolamine A magnetic molecular imprinted polymer microsphere as well as preparation method and application thereof
  • Phenylethanolamine A magnetic molecular imprinted polymer microsphere as well as preparation method and application thereof
  • Phenylethanolamine A magnetic molecular imprinted polymer microsphere as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1. Preparation of Phenylethanolamine A Magnetic Molecularly Imprinted Polymer Microspheres (MMIP)

[0029] (1) Fe 3 o 4 Synthesis of Magnetic Nanoparticles

[0030] Into a 500ml Erlenmeyer flask, pass nitrogen gas for 20min, and add 2g FeCl to it successively 2 4H 2 O, 5.2 g FeCl 3 ·6H 2 O. Dissolve 0.85ml of 12mol / L HCl in 200mL of water, and ultrasonically deoxygenate for 20min. Prepare 250mL of 0.75mol / L NaOH solution and ultrasonically deoxygenate for 20min. Add HCl solution and N to the Erlenmeyer flask in sequence a OH solution, put it into the magnet, and pass nitrogen gas again for 10 minutes to ensure that the Erlenmeyer flask is filled with nitrogen gas, quickly cover and seal. The reaction was carried out in an environment of a water bath at 80° C. with electromagnetic stirring for 2 hours, and a black precipitate was obtained. Separated with an external magnetic field, washed 3 times with deionized water and 2 times with absolute ethanol to...

Embodiment 2

[0037] Characterization of the phenylethanolamine A magnetic molecularly imprinted polymer microspheres prepared in Example 2 and Example 1

[0038] (1) TEM transmission electron microscope analysis

[0039] Use TEM transmission electron microscope to observe the size and shape of magnetic nanoparticles, such as figure 2 (a), (b), (c), where, figure 2 (a) for Fe 3 o 4 TEM images of magnetic nanoparticles, figure 2 (b) and figure 2 (c) are TEM pictures of MMIP prepared in Example 1. It can be seen from the figure that the unmodified Fe 3 o 4 The diameter of the magnetic nanoparticles is about 15nm, and they are very easy to agglomerate. It can be observed from the figure that some large and compact agglomerates have been formed. Compared figure 2 (a), Magnetic microspheres coated with a layer of molecularly imprinted polymers in figure 2 The particle size in (b) and (c) is obviously dozens of times larger, about 300nm, but it shows better dispersion and has an o...

Embodiment 3

[0044] Example 3, Research on the adsorption performance of phenylethanolamine A magnetic molecularly imprinted polymer microspheres

[0045] (1) Comparison of imprinted magnetic microspheres and non-imprinted microspheres

[0046] A standard solution of phenylethanolamine A was prepared, using ultrapure water and PBS buffer solutions of different pH as solvents, and 5 mg of magnetic nanoparticles were adsorbed for the same period of time before HPLC / MS / MS detection. The experimental results show that PA achieves better adsorption effect in PBS buffer solution with pH 8.0. Subsequent experiments all used PBS buffer solution with pH 8.0 as solvent.

[0047] In this embodiment, the adsorption is overnight, so the adsorption time is selected as 12h. With 2% acetic acid-water as the eluent, the elution time is 2h.

[0048] Prepare 50, 100, and 200 μg / L standard solutions of phenylethanolamine A, add 5 mg of MMIP and MNIP magnetic nanoparticles respectively, absorb with 8 mL of ...

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Abstract

The invention discloses a phenylethanolamine A magnetic molecular imprinted polymer microsphere as well as a preparation method and application thereof. The method comprises the following steps: mixing phenylethanolamine A and methacrylic acid, and then pre-polymerizing; adding surface aminated Fe3O4 magnetic nanoparticles, divinyl benzene and 2, 2'-azodiisobutyronitrile into a system after prepolymerization for polymerization reaction; collecting polymers obtained after the polymerization reaction by a magnet, and washing the polymers with acetic acetonitrile liquor, wherein when the cleaning liquid does not have the absorption peak of phenylethanolamine A under ultraviolet detection, the microsphere is obtained, and the ultraviolet absorption peak of phenylethanolamine A in the ultraviolet-visible absorption spectrum is 278nm. According to the invention, a molecular imprinted polymer is combined with Fe3O4, thereby not only effectively preventing Fe3O4 magnetic nanoparticles from being agglomerated and oxidized, but also selectively identifying template molecules as well as analogues thereof by means of cavities in the molecular imprinted polymer which are matched with the steric configuration of the template molecules and have multiple acting points. Therefore, the microsphere has better including and complexing capacity and chemical stability.

Description

technical field [0001] The invention relates to a phenylethanolamine A magnetic molecularly imprinted polymer microsphere, a preparation method and application thereof. Background technique [0002] Phenylethanolamine A, also known as clonpamine (PA), belongs to β-receptor agonists, and its molecular structure is as follows: figure 1 shown. The Ministry of Agriculture has explicitly prohibited the use of phenylethanolamine A in animal feeding in Announcement No. 1519 (2010) "Catalogue of Drugs Prohibited for Use in Feed and Animal Drinking Water". However, driven by profit, there are still some lawbreakers who regard it as a substitute for "clenbuterol" (clenbuterol hydrochloride) and illegally use it to feed animals in feed or breeding, and make huge profits from it. Therefore, it is extremely important to establish a convenient and reliable method for the enrichment and detection of PA and its analogues. [0003] The phenylethanolamine A magnetic molecularly imprinted m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F212/36C08F220/06C08F2/44C08K9/06C08K3/22C08J9/26B01J20/26B01J20/28B01D15/08
Inventor 王培龙苏晓鸥朱若华刘佳贾静
Owner INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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