Method for detecting content of phenylethanolamine A by utilizing CdTe quantum dot

A technology of phenylethanolamine and quantum dots, applied in the field of analytical chemistry, can solve the problems that cannot be used to detect the content of phenylethanolamine A, and achieve the effects of short detection time, low detection limit and low analysis cost

Active Publication Date: 2016-07-06
INST OF AGRI PROD QUALITY SAFETY & STANDARD JIANGXI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the difference in the structure of albuterol and phenylethanolamine A, the mechanism of interaction with CdTe quantum dots is different, the above method cannot be used to detect the content of phenylethanolamine A

Method used

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  • Method for detecting content of phenylethanolamine A by utilizing CdTe quantum dot
  • Method for detecting content of phenylethanolamine A by utilizing CdTe quantum dot
  • Method for detecting content of phenylethanolamine A by utilizing CdTe quantum dot

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Embodiment 1

[0037] A kind of preparation method of the present embodiment CdTe quantum dots, comprises the steps:

[0038] (1) Weigh 0.1276g of tellurium powder and 0.1100g of sodium borohydride respectively, add 1mL of high-purity water, quickly seal the seal with a parafilm, and insert the injection needle to communicate with the outside world at the same time, carry out the stirring reaction for 4 hours under ice-water bath conditions, and let it stand 10min, get NaHTe solution;

[0039] The reaction equation is as follows:

[0040] 4NaBH 4 +2Te+7H 2 O=2NaHTe+Na 2 B 4 o 7 +14H 2 ↑

[0041] (2) Weigh 0.07gCdCl 2 2.5H 2 Dissolve O in 190mL high-purity water, then add 80μL mercaptopropionic acid to form a suspension system;

[0042](3) First use 0.1mol / L NaOH solution to adjust the pH of the suspension system to 11, pass high-purity nitrogen gas for 30 minutes, and add 200 μL of the NaHTe solution under nitrogen protection to control the concentration of Cd in the solution. 2+ ...

Embodiment 2

[0056] A kind of preparation method of the present embodiment CdTe quantum dots, comprises the steps:

[0057] (1) Weigh 0.12g of tellurium powder and 0.1200g of sodium borohydride respectively, add 2mL of high-purity water, quickly seal the seal with a parafilm, and insert the injection needle to communicate with the outside world at the same time, carry out the stirring reaction for 4 hours under ice-water bath conditions, and let it stand 10min, get NaHTe solution;

[0058] (2) Weigh 0.08gCdCl 2 2.5H 2 Dissolve O in 180mL high-purity water, then add 100μL mercaptopropionic acid to form a suspension system;

[0059] (3) First use 0.1mol / L NaOH solution to adjust the pH of the suspension system to 10, pass high-purity nitrogen gas for 30 minutes, and under nitrogen protection, add 500 μL of the NaHTe solution to control the concentration of Cd in the solution. 2+ 、HTe - , The molar ratio of mercaptopropionic acid is 0.9:0.7:2.8 to obtain the CdTe quantum dot precursor;

...

Embodiment 3

[0068] A kind of preparation method of the present embodiment CdTe quantum dots, comprises the steps:

[0069] (1) Weigh 0.13g tellurium powder and 0.1300g sodium borohydride respectively, add 4mL high-purity water, quickly seal the seal with a parafilm, insert the injection needle to communicate with the outside world at the same time, carry out the stirring reaction for 4h under ice-water bath conditions, and let it stand 10min, get NaHTe solution;

[0070] (2) Weigh 0.06gCdCl 2 2.5H 2 O was dissolved in 200mL high-purity water, and then mercaptopropionic acid was added to form a suspension system;

[0071] (3) First use 0.1mol / L NaOH solution to adjust the pH of the suspension system to 12, pass high-purity nitrogen gas for 30 minutes, and under nitrogen protection, add 400 μL of the NaHTe solution to control the concentration of Cd in the solution. 2+ 、HTe - , The molar ratio of mercaptopropionic acid is 0.8:0.6:2.5 to obtain the CdTe quantum dot precursor;

[0072] (...

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Abstract

The invention relates to a method for detecting the content of phenylethanolamine A by utilizing a CdTe quantum dot. The method takes mercaptopropionic acid as a stabilizer; the CdTe quantum dot prepared by a microwave radiation heating method is used as a fluorescent probe; and the method for detecting the content of the phenylethanolamine A is established based on a fluorescent intensity enhancing effect on the CdTe quantum dot by the phenylethanolamine A. The method comprises the following steps: uniformly mixing the CdTe quantum dot with the phenylethanolamine A of a substance to be detected in a phosphate buffering solution; standing for 15 minutes; and detecting the fluorescent intensity of a system by adopting a molecular fluorescence photometer so as to realize trace detection of the phenylethanolamine A. The method has the advantages of high detection sensitivity, simplicity and convenience for operation, short detection time, low analysis cost, relatively low detection limit and relatively high standard recovery rate.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and in particular relates to a method for detecting the content of phenylethanolamine A by using CdTe quantum dots. Background technique [0002] Phenylethanolamine A is a β-receptor agonist, which can make the nutrients in the animal body transfer from fat to muscle, showing a nutrient redistribution effect, and then regulate the material metabolism of the animal body, enhance fat decomposition, promote protein synthesis, and significantly improve carcass. Lean percentage and feed compensation. However, β-receptor agonists tend to accumulate residues in animal tissues, especially viscera. After entering the human body through the food chain, they can cause symptoms such as muscle tremors, arrhythmia, and headaches. In severe cases, they may be life-threatening. In view of the above reasons, my country has listed β-receptor agonists such as phenylethanolamine A, clenbuterol, and phenylethanol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64C09K11/88C01B19/04
CPCC01B19/04C09K11/883G01N21/6428G01N2021/6432
Inventor 张金艳魏益华罗林广邱素艳廖且根胡丽芳魏本华周瑶敏
Owner INST OF AGRI PROD QUALITY SAFETY & STANDARD JIANGXI ACAD OF AGRI SCI
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