Method for simultaneously analyzing and detecting residual veterinary drug compositions in animal tissue
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A technology for animal tissue and animal detection, which is applied in analysis materials, measuring devices, material separation, etc., can solve the problems of complex operation steps and high detection costs, improve detection efficiency, reduce cost consumption, and simplify sample pretreatment and analysis. Effect
Inactive Publication Date: 2014-04-09
INSPECTION & QUARANTINE TECH CENT OF NINGBO ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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However, there are currently no national standard methods for many drugs, such as dapsone, phenylethanolamine, amantadine, rimantadine, etc.
In the existing national standard methods, it is also necessary to use many different methods to detect these compounds, the operation steps are complicated, solid phase extraction columns are required, and the detection cost is high. Therefore, it is necessary to develop a general detection method for Daily sample testing
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[0026] In this example, 18 kinds of quinolones, 22 kinds of sulfonamides, dapsone, phenylethanolamine A, amoxicillin, amantadine, rimantadine, and ethoxyquinoline residues were analyzed simultaneously in animal tissues .
[0027] 1. Materials
[0028] 1.1 Instruments used: ultra-high performance liquid chromatography-tandem mass spectrometry. (Waters Company of the United States); refrigerated centrifuge (SIGMA Company of the United States). Ultrapure water device MILLI-Q (MILLI PORECO, USA). Vortex mixer Votexgenie-2 (Germany). Ultrasonic cleaner 250LH type (KUDOS company.)
[0029] 1.2 Reagents used: methanol, acetic acid, acetonitrile, ethanol (all HPLC grade). 2.0mmol / L ammonium acetate aqueous solution: add 0.154g ammonium acetate to 1000mL water, and mix by ultrasonic. Acetonitrile + ethanol: 4:1, V / V.
[0030] 1.3 Standards used: purity ≥ 95%, purchased from Dr. Ehrenstorfer, Sigma.
[0031] 2. Simultaneous rapid analysis and detection method of multi-component ...
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Abstract
A disclosed method for simultaneously analyzing and detecting residual veterinary drug compositions in animal tissue comprises: performing homogenate on an animal tissue sample by 50% acetonitrile and ethanol, performing ultrasonic extraction and hexane purifying, and performing further precipitation by acetonitrile and ethanol, concentrating, utilizing UPLC-MS/MS to perform multi-reaction monitoring (MRM) determination under the negative ion mode, and quantifying according to a standard curve and an external standard method, detecting and calculating to obtain the content of 46 residual veterinary drug compositions in animal tissue. The method is applicable to present commonly-used veterinary drugs such as 18 kinds of quinolone drugs, 22 kinds of sulfanilamide drugs, dapsone, phenylethanolamine A, amoxicillin, adamantanamine, rimantadine, ethoxyquin and the like, and is capable of performing one-step simultaneous rapid accurate detection, and the application scope of the method is enlarged.
Description
1. Technical field [0001] The present invention relates to a method for analyzing and detecting residues of commonly used veterinary drugs in animal tissues, in particular to 18 kinds of quinolones, 22 kinds of sulfonamides, 2 kinds of antiviral drugs, 1 kind of β-lactams, 1 kinds of residues in animal tissues One kind of sulfone antibacterial drug, one kind of quinoxaline, one kind of β-agonist drug, a new rapid and universal multi-component simultaneous analysis and detection method. 2. Background technology [0002] 18 quinolones (including pipemidic acid, marbofloxacin, pefloxacin, ofloxacin, enoxacin, norfloxacin, ciprofloxacin, enrofloxacin, and monofloxacin , lomefloxacin, orbifloxacin, difloxacin, sarafloxacin, sparfloxacin, cinoxacin, oxolinic acid, nalidixic acid, flumequine); 22 kinds of sulfa drugs (sulfa, acetyl Sulfa, Sulfadiazine, Sulfamethoxine, Sulfathiazole, Sulfapyridine, Sulfamethazine, Trimethoprim, Sulfamethoxypyridazine, Sulfamethoxine, Sulfamethoxazi...
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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 倪梅林钟莺莺葛晓鸣湛嘉俞雪钧
Owner INSPECTION & QUARANTINE TECH CENT OF NINGBO ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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