Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Enzyme-linked immunoassay kit for detecting phenylethanolamine A

An enzyme-linked immunosorbent reagent and phenylethanolamine technology, which is applied in measurement devices, instruments, scientific instruments, etc., can solve the problems that liquid chromatography-tandem mass spectrometry instruments are expensive, not suitable for rapid screening of a large number of samples, and high requirements for inspection personnel. To achieve the effect of simple processing process, simple and time-saving measurement method, and high sensitivity

Inactive Publication Date: 2012-10-17
CHINA INST OF VETERINARY DRUG CONTROL
View PDF5 Cites 14 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the high price of liquid chromatography-tandem mass spectrometry instruments, complex operation and high requirements for inspectors, it is not suitable for rapid screening of a large number of samples

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Enzyme-linked immunoassay kit for detecting phenylethanolamine A
  • Enzyme-linked immunoassay kit for detecting phenylethanolamine A
  • Enzyme-linked immunoassay kit for detecting phenylethanolamine A

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1, the preparation of the enzyme-linked immunosorbent assay kit that detects phenylethanolamine A drug

[0039] 1. Antigen and Antibody Preparation

[0040] 1. Synthesis of immune (antigen)

[0041] Phenylethanolamine A (referred to as PEA, Hangzhou Dean Technology Co., Ltd.) and human serum albumin (referred to as HSA, Sigma, A-1653) were coupled by diazo method to obtain phenylethanolamine A and human serum albumin conjugate ( PEA-HSA for short), as the immunogen (antigen).

[0042]The specific operation is as follows: Dissolve 30 mg of phenylethanolamine A in water, add 2 mL of hydrochloric acid (HCl concentration of 1 mol / L) and 10 mg of zinc powder in sequence, and slowly stir for 1-5 hours to carry out the reduction reaction to obtain aminophenylethanolamine A solution. Add 20mg of sodium nitrite and 200mg of human serum albumin to the aminophenylethanolamine A solution, mix well, stir at 2-8°C for 1-2 hours to carry out diazo coupling reaction, and t...

Embodiment 2

[0066] Embodiment 2, kit sensitivity, specificity, precision and accuracy test

[0067] 1. Sample pretreatment and detection method

[0068] 1. Pretreatment of urine samples

[0069] Take a fresh or frozen-thawed urine sample, centrifuge at 5000g for 5min, and take the supernatant as the test sample.

[0070] 2. Detection method

[0071] Return the kit of Example 1 to room temperature (18°C-30°C) before testing, add water to the H reagent solution to make a washing solution (0.05% Tween 20 phosphate buffer), and use G reagent solution and washing solution to prepare Enzyme-labeled antigen solution (0.1mg / L, when the protein concentration of G reagent solution is 0.1mg / L, it does not need to be diluted with washing solution). Add 50 μl of sample solution or phenylethanolamine A series concentration standard solution to each well of the enzyme-labeled plate prepared in Step 2, 3 of Example 1, then add 100 μl of enzyme-labeled antigen solution, cover the plate with membrane an...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention discloses an enzyme-linked immunoassay kit for detecting phenylethanolamine A. The kit contains a specific antibody of the phenylethanolamine A, wherein the specific antibody is packaged separately. The specific antibody of the phenylethanolamine A is a polyclonal antibody or a monoclonal antibody prepared by adopting a conjugate of the phenylethanolamine A and a carrier protein as immunogen. According to the present invention, an ELISA competition method is adopted to qualitatively or quantitatively detect the residue level of the phenylethanolamine A drug in animal urine, serum, tissue (muscle, liver and kidney), feed, and other samples; the pre-treatment requirements on the sample are low; the animal urine and the animal serum can be detected directly; the tissue samples and the feed can be loaded on the kit after sample liquid extraction; a large number of samples can be concurrently and rapidly detected; the detecting method has characteristics of simpleness and time saving, and the result can be obtained within one hour; and the lowest detection limit of the pig urine sample is 0.5 mug / L. The kit of the present invention has characteristics of high specificity, high sensitivity, high precision and high accuracy, and plays important roles in detections of the phenylethanolamine A residue in animal products.

Description

technical field [0001] The invention relates to an ELISA kit for detecting phenylethanolamine A in the technical field of ELISA and detection and analysis of veterinary drug residues. Background technique [0002] In the modern breeding industry, in order to increase the lean meat mass of animals, reduce the use of feed and reduce costs, criminals illegally use clenbuterol, ractopamine and albuterol and other β-receptor stimulant substances. As this type of prohibited drug has become the key target of government supervision and crackdown, individual lawbreakers have chosen alternatives, among which phenylethanolamine A is the latest β-receptor stimulant substance added to feed that was discovered in 2011. Phenylethanolamine A, also known as "Clonpamine", is a synthetic chemical substance with a molecular formula of C 19 h 24 N 2 o 4 , the chemical structural formula is 2-[4-(4-nitrophenyl)butyl-2-amino]-1-methoxyphenethyl alcohol, which is an isomer of formoterol. It ha...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/544G01N33/577
Inventor 刘智宏汪霞白玉惠金银珍王鹤佳毕言锋徐士新王旻子张聪敏
Owner CHINA INST OF VETERINARY DRUG CONTROL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com