12results about How to "Achieve specific identification" patented technology

The present invention relates to the field of biotechnology detection, and in particular to a primer and a probe and a kit for detecting Salmonella pullorum. The present invention provides a primer and a probe for detecting Salmonella pullorum, wherein the primer includes a primer for detecting Salmonella pullorum ( Salmonella Pullorum , SP) citE2 SP-F and SP-R of a gene target sequence, wherein the probe is modified with a fluorescent reporter group and a fluorescent quencher group. citE2 The primer pair SP‑F and SP‑R that specifically bind to the gene target sequence, combined with the probe for atopic recognition, can accurately and sensitively detect Salmonella pullorum.

The invention discloses an ERA / CRISPR-LFD dual detection system for simultaneously detecting avian metapneumoviruses B and C and application of the ERA / CRISPR-LFD dual detection system, and relates to the technical field of biology. The ERA / CRISPR-LFD dual detection system comprises an ERA amplification reaction primer probe group, crRNA (Complementary Ribonucleic Acid) and an ssDNA-FB Reporter, the ERA amplification reaction primer probe group comprises an upstream primer aMPV / B-F4, a downstream primer aMPV / B-R2, an upstream primer aMPV / C-F3, a downstream primer aMPV / C-R4 and a probe aMPV / C-R2 '''. A novel technical tool which is sensitive, specific, convenient, low in cost and high in stability is provided for on-site rapid typing detection of the avian metapneumoviruses B and C, and the kit has important practical application value and popularization prospect in early discovery, early prevention and control of related diseases of poultry and reduction of loss of the breeding industry.

The invention discloses a fluorescent probe for recognizing lithium ions and a preparation method and application thereof.The method comprises the steps that rhodamine B, HATU and methyl 3-amino-3-methyl-1-butyrate are mixed and subjected to a first-step reaction, a first reactant is obtained, the first reactant, ethyl alcohol and sodium hydroxide are mixed and subjected to a second-step reaction, a second reactant is obtained and subjected to a third-step reaction, and the fluorescent probe for recognizing the lithium ions is obtained. And mixing the second reactant, crown ether, EDCI and HOBT, and carrying out a third-step reaction to obtain the fluorescent probe. According to the method, lithium ions in a complex environment can be efficiently recognized, and raw material waste and resource loss in the production process are avoided.

The invention discloses a light-emitting probe based on a europium-based metal-organic framework and a preparation method and application thereof, and belongs to the technical field of fluorescent sensing materials and food safety detection.The light-emitting probe is a europium-based metal-organic framework material, the chemical formula of the light-emitting probe is Eu (phen) (5-hipa) NO3 <->, phen is 1, 10-phenanthroline ligand, 5-hipa is 5-hydroxyisophthalic acid ligand, and the molecular weight of the Eu (phen) (5-hipa) NO3 <-> is larger than that of Eu (phen) (5-hipa) NO3 <->. The light-emitting probe takes a binuclear Eu cluster as a metal center. According to the luminous probe based on the europium-based metal-organic framework, the synthesized europium-based metal-organic framework material can be used as a bifunctional fluorescent probe, and specific recognition and quantitative detection of carbaryl and enrofloxacin are realized in an aqueous solution; the method has the remarkable effects of high sensitivity, strong anti-interference capability, recyclability and simplicity, convenience and rapidness in operation.

The invention discloses a tyrosine enantiomer sensor and an identification method thereof based on the aggregation-induced electrochemiluminescence effect of chiral pyrene tetracarboxylic acid, and belongs to the field of electrochemiluminescence technology. With 3,4,9,10-pyrene tetracarboxylic acid as the main material, S-3-amino-1-butanol as the chiral ligand, chirality transfer is achieved through an amidation reaction, and under the action of dodecyl ammonium bromide, chloroform and cyclohexane, the monomers are aggregated in micelles through intermolecular π-π stacking interactions, which restricts intramolecular motion, thereby triggering the aggregation-induced electrochemiluminescence effect, and obtaining a pyrene tetracarboxylic acid-S-3-amino-1-butanol assembly, potassium persulfate as a co-reaction reagent, when tyrosine enantiomers are present in the system, electrochemiluminescence signals of different intensities are presented, and a sensing method based on a new electrochemiluminescence system of a pyrene tetracarboxylic acid-S-3-amino-1-butanol assembly is constructed accordingly, and stable identification of tyrosine enantiomers is achieved.

The invention discloses a helicobacter pylori ureB molecular imprinting electrochemical sensor and a preparation method thereof, and belongs to the technical field of biological analysis. The technical problems that in the prior art, helicobacter pylori detection time is long, operation is complex, and dependence on laboratory equipment is high are solved, an electrode substrate is plated with gold to obtain a gold-plated electrode, the gold-plated electrode is treated with ethyl alcohol of 4-aminothiophenol and a PBS buffer solution of a protein cross-linking agent DTSSP to obtain a cleavable covalent bond fixed support electrode, and the cleavable covalent bond fixed support electrode is used for detecting helicobacter pylori. The preparation method comprises the following steps: preparing an ePDA film covered electrode, treating the ePDA film covered electrode with a ureB solution and a DA functional monomer solution to obtain an ePDA film covered electrode, treating the ePDA film covered electrode with an ethanol solution of 2-mercaptoethanol and an acetic acid solution to obtain a molecularly imprinted polymer electrode, and taking the molecularly imprinted polymer electrode as a working electrode to obtain the helicobacter pylori ureB protein molecularly imprinted electrochemical sensor. When the helicobacter pylori ureB molecularly imprinted electrochemical sensor is used for detecting helicobacter pylori ureB, the current response is good, the stability is high, and the operability is high.

This invention discloses a sonodynamic synergistic thrombolysis system and its application. The system includes a tissue plasminogen activator variant, a sonosensitive agent, and an ultrasound source. The tissue plasminogen activator variant is an intravenously administered formulation, the sonosensitive agent is an intravenously infused formulation, and the ultrasound source is used to emit ultrasound during sonosensitive agent administration to activate the agent. This invention significantly improves thrombolysis efficiency and reduces thrombolytic agent dosage and bleeding risk through the dual synergistic effect of TNK-tPA enzymatic hydrolysis and sonodynamic chemical oxidation. The system can significantly improve vascular recanalization rate and blood flow velocity recovery rate, and has high biocompatibility. This invention is applicable to thrombolytic treatment of various thrombotic diseases and has the advantages of simple operation, strong targeting, and wide application range.

This invention discloses an anion-pillared ultraporous membrane, its preparation method, and its application in the separation of low-carbon hydrocarbons. The ultraporous membrane exhibits stable properties and excellent separation selectivity and permeation flux. Preparation method: A polymer with hydrogen-bonded groups is spin-coated onto a substrate, dried to form a film, the substrate is modified, and then an inorganic anion-pillared ultraporous material layer is grown in situ on the substrate surface to obtain the ultraporous membrane. The inorganic anion-pillared ultraporous material layer is formed by the self-assembly of metal ions, inorganic anions, and flexible ligands through coordination bonds; the metal ion is Cu. 2+ Zn 2+ At least one of the following; the inorganic anion is SiF6. 2‑ GeF6 2‑ At least one of the following; the flexible ligand is at least one of 4,4'-bipyridine sulfide and 4,4'-bipyridine sulfoxide.

This invention relates to the field of biomedical technology, and particularly to a responsive RNA delivery system for glioma, its preparation method, and its applications. By fusing the blood-brain barrier-penetrating peptide ANG-2 to mediate cross-blood-brain barrier delivery, and simultaneously combining it with Siglec-15-mediated immune targeting recognition, a dual-targeting mechanism is constructed. This guides the delivery system to accurately identify glioma lesions, significantly increasing the enrichment of the delivery system in the lesion area, laying the foundation for the efficacy of nucleic acid drugs, effectively improving the targeting and effectiveness of treatment, and avoiding damage to normal brain tissue. The dual stimulation release mechanism of acid response and reactive oxygen species response can accurately respond to endogenous stimuli within glioma lesions and cells. Through the synergistic combination of the GO-C14 carrier and charge-reversed polylysine, the loading efficiency of YTHDF2 siRNA is effectively improved, while simultaneously enhancing the cyclic stability of nucleic acid drugs in vivo.

The present application relates to a kind of molecular imprinting glucose sensor for detecting cell glucose metabolism in real time and its preparation method and application, belong to biological sensor technical field.The present application is carbonized with polyvinyl alcohol electrospinning nanofiber, and network-like carbon nano skeleton with good specific surface area and conductivity is obtained, nickel manganese is embedded into carbon nano skeleton, provides active site with uniform distribution, greatly enhances the electron transfer efficiency and electron conduction capacity between molecular imprinting channel and electrode, improves the sensitivity of sensor for detecting glucose.3-amino phenylboronic acid and pyrrole are used as molecular imprinting polymer film monomer, since 3-amino phenylboronic acid has strong affinity for glucose, pyrrole is electrochemically polymerized to form dense polypyrrole, improve the affinity of molecular imprinting channel to glucose, reduce the influence of other interfering substances in cell culture medium during detection, realize the specific recognition of glucose molecule in culture medium.

The invention belongs to the technical field of ion detection, and particularly relates to a fluorescent probe for detecting iodide ions as well as a preparation method and application thereof. The fluorescent probe comprises 1, 3-diphenylbenzo [4, 5] imidazo [1, 2-alpha] pyrazine and copper ions, the 1, 3-diphenylbenzo [4, 5] imidazo [1, 2-alpha] pyrazine is taken as a fluorescent matrix substance, nitrogen atoms in the 1, 3-diphenylbenzo [4, 5] imidazo [1, 2-alpha] pyrazine structure and the copper ions form coordinate bonds, and under the action of a solvent, the 1, 3-diphenylbenzo [4, 5] imidazo [1, 2-alpha] pyrazine fluorescent probe is constructed. 2-[1, 2-alpha] pyrazine and a copper ion binary system are added, and the fluorescent probe is obtained. The fluorescent probe disclosed by the invention has specific recognition on iodide ions and can be used for directly observing and detecting the iodide ions. The lowest detection limit of the fluorescent probe on iodide ions is 6.64 mu M, and the fluorescent probe has the advantages of high sensitivity, good selectivity and simplicity and convenience in operation.

The application discloses a bimodal sensor and a preparation method and application thereof, and the bimodal sensor comprises Fe3O4@TCPP which is surface-modified with mercury ion aptamer; the Fe3O4@TCPP comprises Fe3O4 and tetra (4-carboxyphenyl) porphyrin which is wrapped on the surface of the Fe3O4. The nano probe has low cost, good anti-interference and selectivity, can realize specific identification of mercury ions in food, and then realizes output of fluorescence / colorimetric spectral bimodal detection results, achieves the purpose of self-correction detection, and improves the selectivity, accuracy and efficiency of detection.