Agricultural product pesticide residue detection method

A technology for pesticide residue detection and agricultural products, applied in the field of detection, can solve problems such as complex chemical components, achieve the effect of fast and convenient process, and reduce detection cost

Inactive Publication Date: 2018-02-23
云南技师学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many kinds of pesticides and complex chemical components, and there are many kinds of vegetables on the market, so it is necessary to provide a method that can quickly and easily detect the residues of pesticides in vegetables

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method for detecting pesticide residues in agricultural products, comprising the steps of:

[0024] S1. Take 0.17 parts of monocrotophos, 1.4 parts of chitosan, 3.0 parts of hydroxyethyl acrylate, 1.5 parts of polyethyleneimine, and 4.0 parts of deionized water in a beaker and mix them thoroughly. After deoxidizing with nitrogen, fill it In the splint space formed by two glass slides, 60Co-γ rays were used to irradiate hydroxyethyl acrylate and polyethyleneimine at -78°C to synthesize a film-like imprinted hydrogel with template molecules ;

[0025] S2. Mince the vegetables to be detected; add an extractant for ultrasonic extraction, then add an adsorbent for centrifugal purification, recover the upper extract after purification, blow off the extractant in the eluent; blow off the solvent after the extraction Add a chelating agent to the solution for mixing, then add reduced glutathione and glutathione transferase to catalyze the mixed reaction, after the reaction is...

Embodiment 2

[0031] A method for detecting pesticide residues in agricultural products, comprising the steps of:

[0032] S1. Take 0.21 parts of omethoate, 1.4 parts of chitosan, 3.0 parts of hydroxyethyl acrylate, 1.5 parts of polyethyleneimine, and 4.0 parts of deionized water in a beaker and mix them thoroughly. After deoxidizing with nitrogen, fill it In the splint space formed by two glass slides, 60Co-γ rays were used to irradiate hydroxyethyl acrylate and polyethyleneimine at -78°C to synthesize a film-like imprinted hydrogel with template molecules ;

[0033] S2. Mince the vegetables to be detected; add an extractant for ultrasonic extraction, then add an adsorbent for centrifugal purification, recover the upper extract after purification, blow off the extractant in the eluent; blow off the solvent after the extraction Add a chelating agent to the solution for mixing, then add reduced glutathione and glutathione transferase to catalyze the mixed reaction, after the reaction is com...

Embodiment 3

[0039] A method for detecting pesticide residues in agricultural products, comprising the steps of:

[0040]S1. Take 0.19 parts of phorate, 1.4 parts of chitosan, 3.0 parts of hydroxyethyl acrylate, 1.5 parts of polyethyleneimine, and 4.0 parts of deionized water in a beaker and mix them thoroughly. After deoxidizing with nitrogen, fill it In the splint space formed by two glass slides, 60Co-γ rays were used to irradiate hydroxyethyl acrylate and polyethyleneimine at -78°C to synthesize a film-like imprinted hydrogel with template molecules ;

[0041] S2. Mince the vegetables to be detected; add an extractant for ultrasonic extraction, then add an adsorbent for centrifugal purification, recover the upper extract after purification, blow off the extractant in the eluent; blow off the solvent after the extraction Add a chelating agent to the solution for mixing, then add reduced glutathione and glutathione transferase to catalyze the mixed reaction, after the reaction is comple...

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PUM

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Abstract

The invention discloses an agricultural product pesticide residue detection method. The method comprises the following steps that template molecules, chitosan, hydroxyethyl acrylate, polyethyleneimineand deionized water are taken, imprinted hydrogel with the template molecules is synthesized; pretreatment of to-be-detected vegetables is completed, and a to-be-detected sample solution is prepared;the template molecules of the obtained imprinted hydrogel are removed; a standard Raman spectrogram of the template molecules is made; the imprinted hydrogel obtained after the template molecules areremoved is soaked in the to-be-detected sample solution and fished out, after confocal micro-Raman Spectrum detection is conducted, the obtained Raman Spectrum is compared with the standard Raman spectrogram, and then whether the template molecules are contained in samples or not is judged, and the content range of the molecules is judged preliminarily. Accordingly, the hydrogel with the specificrecognition function is prepared through a molecular imprinting technology, after the to-be-tested samples are adsorbed and enriched by the hydrogel, spectra collection is conducted by means of a portable Raman spectroscope, and whether the samples contain pesticide or not is judged by comparing with the standard Raman spectrogram.

Description

technical field [0001] The invention relates to a detection method, in particular to a detection method for pesticide residues in agricultural products. Background technique [0002] Pesticides play a significant role in promoting the development of forestry and animal husbandry, but the environmental pollution and human health problems caused by the simultaneous use of pesticides are becoming more and more serious. The hazards of pesticide residues have also attracted more and more attention, and many countries have established pesticide residue limits in food. At present, there are many kinds of pesticides with complex chemical components, and there are many kinds of vegetables on the market. Therefore, it is necessary to provide a method that can quickly and easily detect the pesticide residues in vegetables. Contents of the invention [0003] In order to solve the above problems, the invention provides a method for detecting pesticide residues in agricultural products...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/65
Inventor 李学章
Owner 云南技师学院
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