Preparation method of high-sensitivity photoelectrochemical sensor for detecting mucoprotein

A photoelectrochemical and mucin technology, applied in scientific instruments, instruments, measuring devices, etc., can solve problems such as laborious and time-consuming

Active Publication Date: 2020-10-27
UNIV OF JINAN
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Problems solved by technology

However, these methods require complex sample pretreatment and expensive instruments, which are time-consuming and labor-intensive. Therefore, it

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0021] Example 1: Application of Ultrasensitive Photoelectrochemical Sensor in Detection of Mucin 1 (MUC1)

[0022] (1) Deposit a layer of Au nanoparticles on the ITO electrode: first, wash the ITO conductive electrode in acetone, ethanol, and secondary water for 10 min, and insert the cleaned electrode into the deposition solution containing 1% chloroauric acid. Au nanoparticles were deposited on the surface of the ITO conductive electrode at a deposition potential of -0.2 V and a deposition time of 60 s using the potential stripping analysis method. After the deposition was completed, the electrode surface was cleaned with secondary water and dried naturally at room temperature. , to obtain ITO / Au electrodes;

[0023] (2) Using the in-situ growth method to obtain TiO grown on the surface of the ITO / Au electrode in step (1) 2 Particles: insert the ITO / Au electrode into a 25 mL beaker containing 15 mL of ammonium fluorotitanate and boric acid mixture, the concentration of amm...

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Abstract

The invention discloses a preparation method of a high-sensitivity photoelectrochemical sensor for detecting mucoprotein. By growing a composite nano material of titanium dioxide and sulfur indium zinc on the surface of the indium tin oxide conductive electrode modified with the gold nanoparticles, more hairpin DNA chains can be fixed, and detection sensitivity is improved; then, a multi-branch hybrid chain connected with an aptamer and modified with manganese dioxide particles is loaded on the surface of the electrode, has good catalytic reduction capability on hydrogen peroxide, and is usedas a mimic enzyme signal label to consume an electron donor hydrogen peroxide to realize amplification of an analysis signal; through recognition and enzyme digestion of exonuclease, signal amplification of mucoprotein is further realized, so preparation of the photoelectrochemical sensor is completed, and ultra-sensitive and accurate detection of mucoprotein is realized.

Description

technical field [0001] The invention relates to the technical fields of tumor marker detection technology, signal amplification technology of multi-branched hybrid chain and composite nanomaterials, and more specifically relates to a photoelectrochemical sensor for detecting mucin constructed based on the enzyme-like catalytic performance of nanomaterials. Background technique [0002] In recent years, the incidence of cancer in the world has risen sharply, which is a great threat to human health, and early detection of cancer has attracted more and more attention. Mucin is a kind of protein with high molecular weight and high glycosylation. It exists in various malignant tumors and is often highly expressed in breast cancer, ovarian cancer, prostate cancer, pancreatic cancer and other cancers. It is a potential Therefore, it has aroused great interest of scientists in the field of early diagnosis of cancer. So far, several detection methods like enzyme-linked immunosorbent...

Claims

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Application Information

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IPC IPC(8): G01N27/416G01N27/327
CPCG01N27/416G01N27/3278G01N27/3276
Inventor 李正林杨红梅于京华
Owner UNIV OF JINAN
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