Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of highly sensitive photoelectrochemical sensor for detecting microRNA

A photoelectrochemical and sensitive detection technology is applied in the field of photoelectrochemical sensor preparation, which can solve the problems of time-consuming, complex and expensive instruments, and achieve the effects of enhancing sensitivity, accurate detection, and improving analysis and detection signals.

Active Publication Date: 2019-11-05
UNIV OF JINAN
View PDF8 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods are time-consuming, require complex sample pretreatment and expensive instruments, so there is still an urgent need to develop an ultra-sensitive, portable, and low-cost analytical method for detecting microRNA

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Application of ultrasensitive photoelectrochemical sensors in the detection of microRNA-141

[0031] (1) Use Adobe illustrator CS4 software on the computer to design the hydrophobic wax printing pattern of the microfluidic paper chip, print the designed printing pattern on the chromatographic paper through the wax printer, and then put the printed chromatographic paper in the oven, Heat at 130°C for 50 seconds to melt the wax and penetrate the entire thickness of the chromatography paper, forming a hydrophobic area and a hydrophilic working area;

[0032] (2) Design the printing patterns of the working electrode, counter electrode and reference electrode that match the wax printing pattern obtained in step (1) on the computer, and use screen printing technology to print the wax on the wax obtained in step (1). Printed carbon working electrode, carbon counter electrode and Ag / AgCl reference electrode on chromatographic paper;

[0033] (3) Using the in situ gr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a highly sensitive paper-based photoelectrochemical sensor for detecting microRNA. A paper chip is prepared by means of a wax printing technology, gold nano-particles are grownin situ in a hydrophilic working area to achieve functionalization of the paper chip, a cuprous oxide / bismuth sulfide / bismuth vanadate tertiary sensitizer is modified subsequently, and microRNA is captured through a fixed hairpin DNA chain; by means of identification of specificity and digestion effect of duplex-specific nuclease, signal amplification on microRNA is achieved, and then by means ofa multibranched hybrid chain, platinum nanoparticles are embedded into a main part of the chain, branch parts form a chlorhematin / G-tetrad structure, and a DNA concatemer with catalase bological characteristics is formed; signal amplification is further achieved, thus preparation of the photoelectrochemical sensor is completed, and highly sensitive and accurate detection on microRNA is achieved.

Description

technical field [0001] The present invention relates to the technical fields of microRNA analysis and detection technology, multi-branched hybrid chain signal amplification technology, DNA imitation enzyme catalysis technology, paper chip technology and composite nanomaterial technology, more specifically a photoelectrochemical sensor for ultrasensitive detection of microRNA preparation. Background technique [0002] In recent years, the incidence of cancer in the world has risen sharply, which is a great threat to human health, and early detection of cancer has attracted more and more attention. Changes in microRNA can regulate the expression of genes, and it is a potential tumor marker, so the detection of microRNA has attracted widespread attention. High-sensitivity and high-selectivity determination of microRNA is of great significance to gene screening, early diagnosis and treatment of genetic diseases. So far, some detection methods like real-time fluorescent quantit...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/327
CPCG01N27/308G01N27/3278
Inventor 于京华李正林杨红梅胡孟苏颜梅葛慎光
Owner UNIV OF JINAN
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com