Preparation method of highly sensitive photoelectrochemical sensor for detecting microRNA

A photoelectrochemical and sensitive detection technology is applied in the field of photoelectrochemical sensor preparation, which can solve the problems of time-consuming, complex and expensive instruments, and achieve the effects of enhancing sensitivity, accurate detection, and improving analysis and detection signals.

Active Publication Date: 2019-11-05
UNIV OF JINAN
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AI Technical Summary

Problems solved by technology

However, these methods are time-consuming, require complex sample pretreatment and expensive instruments, so there is still an urgent need to develop an ultra-sensitive, portable, and low-cost analytical method for detecting microRNA

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Application of ultrasensitive photoelectrochemical sensors in the detection of microRNA-141

[0031] (1) Use Adobe illustrator CS4 software on the computer to design the hydrophobic wax printing pattern of the microfluidic paper chip, print the designed printing pattern on the chromatographic paper through the wax printer, and then put the printed chromatographic paper in the oven, Heat at 130°C for 50 seconds to melt the wax and penetrate the entire thickness of the chromatography paper, forming a hydrophobic area and a hydrophilic working area;

[0032] (2) Design the printing patterns of the working electrode, counter electrode and reference electrode that match the wax printing pattern obtained in step (1) on the computer, and use screen printing technology to print the wax on the wax obtained in step (1). Printed carbon working electrode, carbon counter electrode and Ag / AgCl reference electrode on chromatographic paper;

[0033] (3) Using the in situ gr...

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Abstract

The invention discloses a highly sensitive paper-based photoelectrochemical sensor for detecting microRNA. A paper chip is prepared by means of a wax printing technology, gold nano-particles are grownin situ in a hydrophilic working area to achieve functionalization of the paper chip, a cuprous oxide/bismuth sulfide/bismuth vanadate tertiary sensitizer is modified subsequently, and microRNA is captured through a fixed hairpin DNA chain; by means of identification of specificity and digestion effect of duplex-specific nuclease, signal amplification on microRNA is achieved, and then by means ofa multibranched hybrid chain, platinum nanoparticles are embedded into a main part of the chain, branch parts form a chlorhematin/G-tetrad structure, and a DNA concatemer with catalase bological characteristics is formed; signal amplification is further achieved, thus preparation of the photoelectrochemical sensor is completed, and highly sensitive and accurate detection on microRNA is achieved.

Description

technical field [0001] The present invention relates to the technical fields of microRNA analysis and detection technology, multi-branched hybrid chain signal amplification technology, DNA imitation enzyme catalysis technology, paper chip technology and composite nanomaterial technology, more specifically a photoelectrochemical sensor for ultrasensitive detection of microRNA preparation. Background technique [0002] In recent years, the incidence of cancer in the world has risen sharply, which is a great threat to human health, and early detection of cancer has attracted more and more attention. Changes in microRNA can regulate the expression of genes, and it is a potential tumor marker, so the detection of microRNA has attracted widespread attention. High-sensitivity and high-selectivity determination of microRNA is of great significance to gene screening, early diagnosis and treatment of genetic diseases. So far, some detection methods like real-time fluorescent quantit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/327
CPCG01N27/308G01N27/3278
Inventor 于京华李正林杨红梅胡孟苏颜梅葛慎光
Owner UNIV OF JINAN
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