Photo-induced electrochemical immunosensor for detecting cancer cells based on self-assembled organic semiconductor material PC05 nanowire structure
An organic semiconductor and photoelectrochemical technology, applied in the field of quantitative detection of tumor markers, can solve the problems of complex operation, cumbersome production steps, and time-consuming detection process, and achieve good biocompatibility and enhanced photoelectric signal effects
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[0013] In order to further understand the present invention, carry out according to the technical scheme of the present invention in conjunction with embodiment, provide specific implementation mode:
[0014] (1) Deposition of gold nanoparticles: The conductive glass is indium tin oxide glass (ITO). The conductive glass was cut into strips of 4.0×0.5 cm, and ultrasonically cleaned with acetone solution, double distilled water and absolute ethanol for 5 min, and the Conductive glass is placed in 5.0mmol / L HAuCl 4 In the solution, gold nanoparticles were deposited by cyclic voltammetry, the deposition potential was -1.5~0 V, the number of scan cycles was 20, and the scan rate was 0.1 V / s;
[0015] (2) Preparation of AuNPs-PC05: Weigh 0.00474 g of PC05 and add it to 3.0 mL of tetrahydrofuran, let it stand for 2 minutes, add 3.0 mL of ultrapure water to the above solution, let it stand for 10 minutes, and put the above mixture at 8000 rpm Centrifuge for 2 min to make the final vo...
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