Photo-induced electrochemical immunosensor for detecting cancer cells based on self-assembled organic semiconductor material PC05 nanowire structure

An organic semiconductor and photoelectrochemical technology, applied in the field of quantitative detection of tumor markers, can solve the problems of complex operation, cumbersome production steps, and time-consuming detection process, and achieve good biocompatibility and enhanced photoelectric signal effects

Active Publication Date: 2019-05-03
UNIV OF JINAN
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Problems solved by technology

At present, among the PEC immunosensors that have been constructed for detecting tumor markers, most of the work is to spatially separate the working area to distinguish the signals generated by different tumor markers, resulting in cumbersome manufacturing steps, complicated operations, and difficult detection. Process time-consuming and other issues

Method used

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Embodiment Construction

[0013] In order to further understand the present invention, carry out according to the technical scheme of the present invention in conjunction with embodiment, provide specific implementation mode:

[0014] (1) Deposition of gold nanoparticles: The conductive glass is indium tin oxide glass (ITO). The conductive glass was cut into strips of 4.0×0.5 cm, and ultrasonically cleaned with acetone solution, double distilled water and absolute ethanol for 5 min, and the Conductive glass is placed in 5.0mmol / L HAuCl 4 In the solution, gold nanoparticles were deposited by cyclic voltammetry, the deposition potential was -1.5~0 V, the number of scan cycles was 20, and the scan rate was 0.1 V / s;

[0015] (2) Preparation of AuNPs-PC05: Weigh 0.00474 g of PC05 and add it to 3.0 mL of tetrahydrofuran, let it stand for 2 minutes, add 3.0 mL of ultrapure water to the above solution, let it stand for 10 minutes, and put the above mixture at 8000 rpm Centrifuge for 2 min to make the final vo...

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Abstract

The invention discloses a photo-induced electrochemical immunosensor for detecting cancer cells based on the self-assembled organic semiconductor material PC05 nanowire structure. The length of a nanowire is regulated by using a simple self-assembling method and the nanowire with the most suitable length is selected. The organic semiconductor nanowire has the perfect crystal structure to realize the charge transport well. Meanwhile, gold nanoparticles are deposited on the electrode by electrodeposition and the Fermi level of gold matches the PC05 nanowire well, so that the photoelectric signalof the sensor is enhanced. Moreover, because of the PC05 as a p type semiconductor, the dissolved oxygen in the detection solution can consume the excited electrons in photoelectric testing.

Description

technical field [0001] The invention relates to the field of quantitative detection of tumor markers, more specifically to the photoelectrochemical detection of cancer cells based on the self-assembled organic semiconductor material Cobalt(II)-meso-tetra(4-carboxyphenyl)porphyrin (PC05) nanowire structure Construction of the immunosensor. Background technique [0002] In recent years, the morbidity and mortality of cancer have gradually increased, and this trend is still increasing. Cancer cells refer to a new organism produced by some cells with the potential to divide under carcinogenic factors. In addition to their own uncontrolled growth, cancer cells can also invade the surrounding normal tissues and even transfer to other parts of the body through the body's circulatory system or lymphatic system, thereby causing body diseases and even death. Therefore, it is urgent to develop a sensor with high sensitivity and stability to realize the early diagnosis of cancer. [...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/30G01N27/327G01N33/574
Inventor 颜梅苗培张丛丛于京华张晶秦诚坤赵悦英
Owner UNIV OF JINAN
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