A method for detecting pesticide residues in agricultural products
A technology for pesticide residue detection and agricultural products, applied in the field of detection, can solve problems such as complex chemical components, achieve the effect of fast and convenient process, and reduce detection cost
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Embodiment 1
[0023] A method for detecting pesticide residues in agricultural products, comprising the steps of:
[0024] S1. Take 0.17 parts of monocrotophos, 1.4 parts of chitosan, 3.0 parts of hydroxyethyl acrylate, 1.5 parts of polyethyleneimine, and 4.0 parts of deionized water in a beaker and mix them thoroughly. After deoxidizing with nitrogen, fill it In the splint space formed by two glass slides, 60Co-γ rays were used to irradiate hydroxyethyl acrylate and polyethyleneimine at -78°C to synthesize a film-like imprinted hydrogel with template molecules ;
[0025] S2. Mince the vegetables to be detected; add an extractant for ultrasonic extraction, then add an adsorbent for centrifugal purification, recover the upper extract after purification, blow off the extractant in the eluent; blow off the solvent after the extraction Add a chelating agent to the solution for mixing, then add reduced glutathione and glutathione transferase to catalyze the mixed reaction, after the reaction is...
Embodiment 2
[0031] A method for detecting pesticide residues in agricultural products, comprising the steps of:
[0032] S1. Take 0.21 parts of omethoate, 1.4 parts of chitosan, 3.0 parts of hydroxyethyl acrylate, 1.5 parts of polyethyleneimine, and 4.0 parts of deionized water in a beaker and mix them thoroughly. After deoxidizing with nitrogen, fill it In the splint space formed by two glass slides, 60Co-γ rays were used to irradiate hydroxyethyl acrylate and polyethyleneimine at -78°C to synthesize a film-like imprinted hydrogel with template molecules ;
[0033] S2. Mince the vegetables to be detected; add an extractant for ultrasonic extraction, then add an adsorbent for centrifugal purification, recover the upper extract after purification, blow off the extractant in the eluent; blow off the solvent after the extraction Add a chelating agent to the solution for mixing, then add reduced glutathione and glutathione transferase to catalyze the mixed reaction, after the reaction is com...
Embodiment 3
[0039] A method for detecting pesticide residues in agricultural products, comprising the steps of:
[0040]S1. Take 0.19 parts of phorate, 1.4 parts of chitosan, 3.0 parts of hydroxyethyl acrylate, 1.5 parts of polyethyleneimine, and 4.0 parts of deionized water in a beaker and mix them thoroughly. After deoxidizing with nitrogen, fill it In the splint space formed by two glass slides, 60Co-γ rays were used to irradiate hydroxyethyl acrylate and polyethyleneimine at -78°C to synthesize a film-like imprinted hydrogel with template molecules ;
[0041] S2. Mince the vegetables to be detected; add an extractant for ultrasonic extraction, then add an adsorbent for centrifugal purification, recover the upper extract after purification, blow off the extractant in the eluent; blow off the solvent after the extraction Add a chelating agent to the solution for mixing, then add reduced glutathione and glutathione transferase to catalyze the mixed reaction, after the reaction is comple...
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