159 results about "Rhodamine derivative" patented technology

The invention provides a novel rhodamine fluorescence probe, and belongs to the field of function materials. The novel rhodamine fluorescence probe provided by the invention is characterized in that rhodamine B (RhB) is used as a raw material and is modified by using diamine so as to prepare rhodamine lactam, further the rhodamine lactam reacts with maleic anhydride (MAH) and amantadine so as to obtain the rhodamine fluorescence probe, and the novel rhodamine fluorescence probe contains multiple amido functional groups and carbon-carbon double bonds, wherein the multiple amido functional groups can react with metal ions, and the carbon-carbon double bonds can react with Hg<2+>. After the spiral rhodamine lactam compound is combined with heavy metal particles, the protonation of lactam nitrogen atoms can result in the reduction of the charge density of the nitrogen atoms, thus the cracking of a C-N chemical bond at the spiral center is triggered, so as to generate the fluorescence change and the color change. The obtained rhodamine derivative has relatively good recognition capability on mercury ions and iron ions in a water solution, the detection limit of the obtained rhodamine derivative on the iron ions reaches 8.3ppm, and the detection limit of the obtained rhodamine derivative on the mercury ions reaches 4.7ppm. The prepared rhodamine derivative can serve as the fluorescence probe and is widely applied to the fields such as environmental engineering, detection and the like.

The invention relates to rhodamine derivatives and their preparation method and use. The rhodamine derivatives have a general formula I and can be utilized for determination of Fe<3+>. The preparation method of the rhodamine derivatives comprises that an intermediate produced by the reaction of rhodamine B and ethylenediamine is utilized as a raw material and undergoes a condensation reaction with acetylacetone or ethyl acetoacetate in the presence of ceric ammonium nitrate as a catalyst to produce the rhodamine derivatives. Results of an analysis of a mixed-ion interference-resistant iron-ion specific identification capacity of the rhodamine derivatives and an analysis of a living cell condition adaptation capacity of the rhodamine derivatives show that the rhodamine derivatives have strong iron ion specificity, a strong other cation-resistant capacity and a strong living cell condition adaptation capacity, wherein iron ion concentration detection sensitivity is 1*10<-6>; reaction stabilization time is 20 minutes; and a pH value of a Tris-HCl buffer solution is 5.5 or 6.0. The rhodamine derivatives can be utilized as iron ion induction agents for a non-invasive micro-metering system and realizes determination of Fe<3+> concentration, a Fe<3+> flowing speed and a Fe<3+> motion direction.

The invention relates to a silicon-based rhodamine derivative, which is represented by a biological structural formula shown in the description. The invention also relates to a preparation method of the silicon-based rhodamine derivative, and applications of the silicon-based rhodamine derivative in preparation of cell dye, biological dye, biological molecule / particle fluorescent marker, and the like. The provided synthesis method of silicon-based rhodamine derivative has the advantages of high yield, simple reaction conditions, convenient operation, capability for massive production, and wide substrate selection range.

The invention discloses visual, portable and quick mercury ion detection test paper and a preparation method and application thereof. The preparation method of the test paper comprises the following steps: firstly preparing rhodamine hydrazide, carrying out etherification and Heck reaction on hydroxyl on filter paper so as to introduce in required isosulfocyanate radical end group, grafting the prepared rhodamine hydrazide onto the filter paper to finally obtain the mercury ion detection test paper. The prepared detection test paper has the advantages that a rhodamine derivative fluorescence probe and the filter paper are chemically bonded, the structure is firm, the reaction speed is high, the detection limit is 10mu m, the selectivity is good, the influences from other various coexisting ions are avoided, the detection environment is an organic-solution-free total aqueous solution system containing mercury ions, the pollution of other organic solvents to the environment is reduced; and under the irradiation of an ultraviolet lamp, the test paper can realize visual detection; the detection measures are convenient and can be performed on site; and therefore, the test paper and the preparation method of the test paper have great application prospect in the field of water pollution caused by mercury ions.

The invention provides a rhodamine fluorescent probe capable of detecting and separating heavy metal ions, and a preparation method of the rhodamine fluorescent probe, belonging to the field of a functional material. The fluorescent probe is prepared by comprising the following steps: modifying rhodamine B (RhB) serving as raw material so that the terminal has an azobenzene group; preparing the rhodamine fluorescent probe capable of detecting metal mercury ions and having a relatively low detection limit. By utilizing subjective-objective clathration action of azobenzene and cyclodextrin and taking the cyclodextrin magnetic nanoparticles prepared in advance as an adsorbent, the clathrated fluorescent probe and the detected metal ions can be separated from a solution under the action of an external magnetic field; furthermore, by utilizing the photoreaction property of the azobenzene group, the removal of heavy metal ions and the repeated use of the cyclodextrin magnetic nanoparticles can be realized. The rhodamine derivative has relatively good identification capability to mercury ions in the aqueous solution, thus being used as a mercury ion detection and removal agent. As the fluorescent probe, the rhodamine derivative can be widely applied to the fields of environmental engineering, analysis and detection and the like.

The invention discloses a preparation method and an application of GSH (glutathione) water-soluble fluorescent probe based on rhodamine. A structural formula of the fluorescent probe is represented in the specification. The invention provides a water-soluble fluorescent probe for detecting GSH with high selectivity on the basis of rhodamine derivatives. 1,2,4-trimellitic anhydride, 3-diethylaminophenol, hydrazine hydrate and glyoxal are taken as raw materials to synthesize the fluorescent probe. On one hand, the rhodamine structure is modified with carboxyl, and water solubility of the probe can be greatly improved; on the other hand, a hydrolysis-addition reaction is performed between SH of an analyte and aldehyde groups of the probe, and GSH, Cys and Hcy are distinguished. The fluorescent probe is the first one capable of efficiently distinguishing GSH, Cys and Hcy in a 100% aqueous solution on the basis of the rhodamine derivatives. The probe shows quite high sensitivity and good selectivity on GSH, and can be applied to cell imaging and detect intracellular GSH.

The invention discloses a rhodamine derivative and a preparation method and application thereof. The rhodamine derivative is prepared by reacting rhodamine hydrazide and 4-chloro-7-nitro-2,1,3-benzoxaoxadiazole in the presence of an acid binding agent. The Cu<2+> and Fe<3+> can be respectively detected at high selectivity and high sensitivity. The designed and synthesized structure is short in synthetic route and mild in reaction conditions, the obtained derivative can be used for an aqueous phase system, and the ultraviolet-visible absorption spectrum and fluorescence spectrum are insensitive to the acid-base property of the system.

The invention discloses a preparation method and application of a near infrared GSH (glutathione) fluorescent probe. The structural formula of the fluorescent probe is shown as the accompanying drawing. On one hand, the Rhodamine wavelength is increased by 1,3,3-Trimethyl-2-(formylmethylene)indoline, so that the wavelength is prolonged to be 750nm; on the other hand, SH of an analyte and an aldehyde group of the probe take an addition-hydrolysis reaction for realizing GSH, Cys (cysteine) and Hcy(homocysteine) distinguishing. The near infrared GSH fluorescent probe is the first near infrared fluorescent probe which is based on rhodamine derivatives and can efficiently distinguish the GSH, the Cys and the Hcy; the probe shows high sensitivity on the GSH. When the pH value is 6.0 to 8.0, the determination on the GSH by the fluorescent probe is not influenced; the fluorescent probe and the GSH response is fast; the probe cannot be interfered by other biological mercaptan (Cys and Hcy) and other 19 kinds of amino acids; good selectivity is shown. More importantly, the near infrared GSH fluorescent probe can be applied to optical imaging and detection of GSH in cells and tissues.

The invention discloses a method for preparing a water-soluble mercury ion fluorescence probe on the basis of rhodamine and application of the water-soluble mercury ion fluorescence probe. A structural formula of the Hg<2+> fluorescence probe are shown, and the water-soluble mercury ion fluorescence probe is synthesized from 1, 2, 4-trimellitic anhydride, 3-diethylamino phenol and hydrazine hydrate. The method and the application have the advantages that the water-soluble Hg<2+> fluorescence probe prepared by the aid of the method is simple in structure, is based on rhodamine derivatives and is the first fluorescence probe for detecting Hg<2+> in 100% aqueous solution by the aid of rhodamine lactam derivatives; ring opening of rhodamine lactam structures can be carried out under the induction effects of the Hg<2+> in systems, intense fluorescence can be generated, and the water-soluble mercury ion fluorescence probe is high in Hg<2+> detection sensitivity; the fluorescence probe is excellent in Hg<2+> selectivity and is almost free of interference of other positive ions; the Hg<2+> can be measured by the fluorescence probe without disturbance when pH (potential of hydrogen) values range from 5.0-8.0, the fluorescence probe and the Hg<2+> can act quickly, and the response time is shorter than 3 minutes; the probe further can be applied to measuring Hg<2+> in environmental water samples and carrying out fluorescence imaging on Hg<2+> in biological cells.

The invention discloses a preparation method of a blotting membrane for Hg<2+> detection. The preparation method comprises the following steps: designing and synthesizing Rhodamine derivative with special developing function to Hg<2+>; and opolymerizing on a support polyvinylidene fluoride membrane by using target metal ion as a template, ethylene dimethacrylate as a crosslinking agent, azodiisobutyronitrile as a radical initiator, and the synthesized Rhodamine derivative as a function monomer to obtain the blotting membrane for mercury ion detection. The characteristic that the ionic blotting technology has high selectivity to the template ion is adopted by the invention to combine with the simplicity of a colorimetric / fluorescence chemical sensor, a colorimetric / fluorescence chemical sensing blotting membrane with high selectivity to Hg<2+> is prepared, and a fast and high-speed new scene detection method with high sensitivity and high selectivity is hopefully provided for heavy metal ions in environment and food.

The invention discloses N-(2,4-dinitrophenyl)-rhodamine B hydrazide and a preparation method and an application of the N-(2,4-dinitrophenyl)-rhodamine B hydrazide. The rhodamine B derivative provided by the invention is N-(2,4-dinitrophenyl)-rhodamine B hydrazide, the structural formula of which is I. The N-(2,4-dinitrophenyl)-rhodamine B hydrazide, is colorless and free from fluorescence, but can selectively react with Cu<2+> ions in chromogenic and fluorescent opening reaction. The colorless substance generates a system with excellent optical performance, so that the detection sensitivity and selectivity of Cu<2+> ions are greatly improved. Therefore, the N-(2,4-dinitrophenyl)-rhodamine B hydrazide is appropriate for high selectivity and high sensitivity detection for the Cu<2+> ions. Detection can be carried out by a light absorption spectrum and a fluorescence spectrum. (FORMULA I).

The invention belongs to the technical field of a functional material, and in particular relates to a modified SBA-15 mesoporous material as well as a preparation method and an application thereof. The mesoporous material is an SBA-15 mesoporous material modified by trimethoxy-[(3-ethylene oxide methox)yl propyl] silane and rhodamine B derivative, and the modified mesoporous material is prepared by the following steps: firstly, substituting silicon hydroxyl of an SBA-15 mesoporous material which is prepared in advance with a substitute (as shown in Specification), and then, carrying out a cycloaddition reaction with thio rhodamine B hydrazide (as shown in Specification), so as to obtain the modified SBA-15 mesoporous material with chemical structural formula as shown in Specification. The modified SBA-15 mesoporous material, as a hybridized mesoporous fluorescent adsorbent showing efficient detection and a capacity of removing mercury ions in a water body and integrating multiple functions, can simultaneously carry out detection and recovery processes on dangerous chemical substances in a water solution, an air system, and in particular in body blood or tissue.

The invention relates to a hybridization probe for fluorescent detection of copper ions in a water phase and a preparation method thereof. The hybridization probe is prepared by grafting a fluorescence detection group, i.e., a rhodamine derivative, into a mesoporous silica nanosphere inorganic matrix via a covalent bond and carrying out surface polyethylene glycol (PEG) modification, and can realize fluorescent detection of copper ions in the water phase. The preparation method for the hybridization fluorescent nanometer probe is mild in reaction conditions and simple in synthesis steps. The hybridization fluorescent nanometer probe is applicable to detection of copper ions in water, has good anti-interference capability, good stability and high selectivity, can realize detection of a single copper ion, and is free of influence by co-existing ions, and fluorescence and color changes of the probe are visible to naked eyes. Moreover, the hybridization fluorescent nanometer probe is applicable to fluorescence imaging of Cu<2+> in living cells, and has potential application value in fluorescent detection of Cu<2+> in a living body and in early diagnosis and pathogenesis research of diseases related to Cu<2+>.

The invention discloses an application of a rhodamine B derivative in nitrite ion detection, and the rhodamine B derivative is the rhodamine B derivative by connecting five-membered spirolactam in molecule with 1,2-phenylenediamine. The rhodamine B derivative for nitrite detection is characterized in that 1,2-phenylenediamine is reacted with nitrite under acidic condition, and ring opening of the the rhodamine five-membered spirolactam in molecule is initiated, and a substance with the magenta color and strong fluorescence is generated. The invention has the beneficial effect that intermolecular five-membered rhodamine B spirolactam-o-phenylendiamine is reacted with nitrite to generate the substance possessing color and emission fluorescence. The color development reaction can be used in the fields that 1) nitrite existence can be visually measured; 2) the measurement of the fluorescent emission intensity or visible light absorption intensity of the reaction system through an analysis apparatus enables quantitative determination of the content of the nitrite in the sample.

It is an object of the present invention to provide an ophthalmic lens characterized in that it is capable of developing a color at a position where a light is irradiated, and to provide a marking method of the ophthalmic lens. It is another object of the present invention to provide an ophthalmic lens, which can form a clear and easily readable mark, without causing problems such as discoloration of the mark, caused by cleaning of the lens by rubbing, or deterioration of the mechanical strength of the lens, and to provide a marking method of the ophthalmic lens. The ophthalmic lens of the present invention is obtained by a polymerization of a polymerizable composition of monomers, which contains an alkyl (meth)acrylate as an essential polymerizable component, and this polymerization is carried out while the rhodamine derivative is present in the composition.

The present invention is to provide a colored composition having higher heat resistance compared with conventional colored compositions. The present invention further relates to: “a polymer having a monomer unit derived from a monomer which has (i) a cationic rhodamine derivative having, as an counter anion, an anion including an aryl group having an electron-withdrawing substituent and an anion group and (ii) an ethylenically unsaturated bond”, “a monomer which has (i) a cationic rhodamine derivative having, as an counter anion, an anion including an aryl group having an electron-withdrawing substituent and an anion group and (ii) an ethylenically unsaturated bond”, “a colored composition comprising the above-described polymer or the monomer”, and “a colored composition for a color filter comprising the above-described polymer or the monomer”.

The present invention relates to the use of the photoactivable derivatives for the photodynamic treatment for the selective destruction and / or inactivation of immunologically reactive cells without affecting the normal cells and without causing systemic toxicity for the patient, wherein appropriate intracellular levels of said derivatives are achieved and irradiation of a suitable wavelength and intensity is applied.

The invention discloses a hydrogen-peroxide-enhanced fluorescent probe based on Rhodamine derivatives, having a chemical structural formula shown as in formula (1) which is shown the description. The hydrogen-peroxide-enhanced fluorescent probe based on Rhodamine derivatives is low in price, easy to obtain and convenient to use, is capable of specifically reacting with hydrogen peroxide to generate a Rhodamine derivative having greater fluorescence emitting capacity, is never disturbed by other related oxides during the detection of hydrogen peroxide, has good selectivity for hydrogen peroxide, and can detect hydrogen peroxide in live cells and live animals accurately.

The invention relates to a preparation method of a mercury ion rate probe based on a rhodamine derivative and a quantum dot fluorescent microsphere. The preparation method comprises the steps of firstly, preparing a water-soluble quantum dot (CdTe:Zn) by using a water-phase synthesis method, and synthesizing a silicon dioxide nanoparticle by using a reverse-phase microemulsion method, wherein Zn<2+> is doped into the water-soluble quantum dot, the surface of the water-soluble quantum dot is provided with mercaptopropionic acid (MPA), and the quantum dot is doped into the silicon dioxide nanoparticle; then, preparing a lactamized rhodamine derivative with specific recognition capability for Hg<2+>, and modifying siloxy through aminopropyltriethoxysilane (APS) on the basis, so that the lactamized rhodamine derivative is connected with the silicon dioxide nanoparticle; and finally, grafting the rhodamine derivative onto the surface of the silicon dioxide nanoparticle to construct the mercury ion rate probe based on FRET. By using the probe, not only is the interference of the external environment to the optical property of the quantum dot reduced, but also the distance from an energy donor (the quantum dot) to an energy acceptor (the rhodamine derivative) is shortened, the energy transfer efficiency of FRET is increased, and furthermore the mercury ion detection sensitivity is enhanced.

The invention discloses a rhodamine B derivative containing an 8-aminoquinoline group, a preparation method, an application and a method for carrying out fluorescence analysis on Cr<3+> and Cu<2+>. The rhodamine B derivative containing the 8-aminoquinoline group is capable of selectively and quantitatively detecting chromium ions (Cr<3+>) in acetonitrile, and is capable of selectively and quantitatively detecting copper ions (Cu<2+>) in ethanol and generating an obvious fluorescence increment effect. The rhodamine B derivative containing the 8-aminoquinoline group is simple in synthesis method, and is capable of quantitatively detecting different heavy metal ions Cr<3+> and Cu<2+> in different solvents at high selectivity and high sensitivity, is a dual-functional fluorescence probe, and has a good application prospect in the fields of environment analysis and detection.

Compounds of formula (I)wherein: one of R1, R2, R3, R4 and (R10)n represents halogen and each remaining R1, R2, R3, R4 and R10 group is independently selected from hydrogen, halogen, amino, acylamino, dialkylamino, cycloalkylamino, azacycloalkyl, alkylcycloalkylamino, aroylamino, diarylamino, arylalkylamino, aralkylamino, alkylaralkylamino, arylaralkylamino, hydroxy, alkoxy, aryloxy, aralkyloxy, mercapto, alkylthio, arylthio, aralkylthio, carboxyl, alkoxycarbonyl, aryloxycarbonyl, aralkoxycarbonyl, carbamoyl, alkylcarbamoyl, dialkylcarbamoyl, cyano, hydroxysulfonyl, amidosulfonyl, dialkylamidosulfonyl, arylalkylamidosulfonyl, formyl, acyl, aroyl, alkyl, alkylene, alkenyl, aryl, aralkyl, vinyl, alkynyl, and corresponding substituted groups; m=0-1; n=1-4, A is nil, O, or NH; R9 is alkylene; -Z is H, amino, dialkylamino, or a trialkylamino salt; X− is an anion; R5, R6, R7 and R8 are independently H or C1-C6 alkyl or R1 in combination with R5 or R6, or R2 in combination with R5 or R6, or R3 in combination with R7 or R8, or R4 in combination with R7 or R8 represents alkylene, are useful alone or with a pharmaceutically acceptable carrier, as intermediates and as bactericides, antiviral agents and in treatment of immunologic disorders.

The invention discloses a hydrogen peroxide detecting fluorescent probe ACR, and belongs to the technical field of analytical chemistry. The hydrogen peroxide detecting fluorescent probe uses a rhodamine derivative as a parent and 2-azide methyl aromatic formic acid ester as a switch, and the chemical structural formula of the hydrogen peroxide detecting fluorescent probe is shown in the specification. The hydrogen peroxide detecting fluorescent probe is simple to synthesize and easy to use, can be specifically reacted with hydrogen peroxide to release fluorescence, and has good hydrogen peroxide selectivity, and in the hydrogen peroxide detection process in the presence of other related oxides, the hydrogen peroxide detecting fluorescent probe has good anti-interference performance, and can detect hydrogen peroxide in living cells.

The invention discloses an application of a rhodamine B derivative containing methyl chloride carbonyl piperazine as a cellular mitochondrion fluorescence coloring agent. The rhodamine B derivative can be used as the cellular mitochondrion fluorescence coloring agent for high-selectivity long-term stability label of the cellular mitochondrion. The rhodamine B derivative enters the cellular mitochondrion after incubating with different cells. The rhodamine B derivative has long retention time in the cellular mitochondrion, and has high stability, is not easily optically quenched, and does not react with active substance in the cells. The rhodamine B derivative can be used for specifically and stably labeling different cellular mitochondria. The rhodamine B derivative can be used as a fluorescent label substance for different cells, and can be used for long-time labeling and tracking the mitochondria in the cells.

The invention discloses a reaction type cysteine probe and a preparation method thereof. According to the preparation method, rhodamine derivatives are taken as fluorophores, and an acrylate structure is taken as a specific reaction group of cysteine; the probe is very weak in fluorescent light, but the fluorescent light of the probe is enhanced after the probe reacts with the cysteine, so that the probe can be used for detecting the cysteine. The probe can more rapidly react with the cysteine in a PBS 7.4 (30% ethanol) system, and has good selectivity and higher sensitivity; the probe can be applied to fluorescence detection, visual qualitative detection and cell imaging detection which are carried out for determining the content of the cysteine in biological and environmental systems.

It is an object of the present invention to provide an ophthalmic lens characterized in that it is capable of developing a color at a position where a light is irradiated, and to provide a marking method of the ophthalmic lens. It is another object of the present invention to provide an ophthalmic lens, which can form a clear and easily readable mark, without causing problems such as discoloration of the mark, caused by cleaning of the lens by rubbing, or deterioration of the mechanical strength of the lens, and to provide a marking method of the ophthalmic lens. The ophthalmic lens of the present invention is obtained by a polymerization of a polymerizable composition of monomers, which contains an alkyl (meth)acrylate as an essential polymerizable component, and this polymerization is carried out while the rhodamine derivative is existed in the composition.

To solve the problems caused by using rhodamine derivatives and 9-trimethylbenzyl-10-methylacridine ion that take S and Se as the bridge atoms as the photosensitive agents, the invention provides a novel nitrogen-containing cation chromophore and a preparation method thereof. The compound has the characteristics of long absorption wavelength, long excitation wavelength, high quantum yield and long exiting time of excited triplet state generated by absorbing photons, easy water-solubility, and incapability of coordinating with metal catalysts. The preparation method comprises the following steps: making m-bromoaniline derivatives carry out Ullmann C-N coupling reactions with excess primary amine in the presence of a CuI catalyst so as to obtain a reaction product A; making the reaction product A carry out Ullmann C-N coupling reactions with m-iodoaniline derivatives in the presence of KN(Si(CH3)3)2 so as to obtain a reaction product B; making the reaction product B carry out Bayer condensation reactions with benzaldehyde derivatives in the present of a ferric trichloride catalyst or a zinc dichloride catalyst to enclose the ring so as to obtain a reaction product C; and finally carrying out a dehydration treatment on the reaction product C with 2,3-dicyano-5,6-dichlorobenzoquinone (DDQ) so as to obtain the target product.

The invention discloses a copper ion ratio type fluorescent probe based on a rhodamine derivative and a preparation method and application. The chemical formula of the fluorescent probe is C41H45N5O2.A rhodamine B hydrazide intermediate is firstly prepared, then an ethanol solution of the rhodamine B hydrazide intermediate and an ethanol solution of 1,3,3-trimethyl-2-(formylmethylene)indoline arerespectively prepared, the ethanol solution of 1,3,3-trimethyl-2-(formylmethylene)indoline is dropwise added into the ethanol solution of the rhodamine B hydrazide intermediate for carrying out a reaction, and the distillation and purification is performed to obtain the fluorescent probe. The fluorescent probe is applied to detect Cu<2+>. The fluorescent probe can specifically recognize Cu<2+>, the anti-interference ability is strong, the detection limit is low, the sensitivity is high, the fluorescent probe can be well applied to the Cu<2+> detection in the environment or organisms, and thefluorescent probe has important application values; and the preparation method of the fluorescent probe is simple, and the operability is strong.

The invention discloses an up-conversion fluorescent probe rhodamine derivative and an application thereof. The preparation method comprises the following steps: performing a reaction on rhodamine B and ethylenediamine in a nitrogen gas atmosphere and an organic solvent to obtain rhodamine-ethylenediamine; and performing a reaction on the rhodamine-ethylenediamine and phenyl isothiocyanate to obtain the up-convertion fluorescent probe rhodamine derivative. The up-conversion fluorescent probe rhodamine derivative provided by the invention has the high-efficiency down-conversion fluorescence-enhanced response recognition characteristics for mercury ions and has the high-efficiency up-conversion fluorescence-enhanced response recognition characteristics for the mercury ions when used as a fluorescent probe.

The invention discloses a preparation method of a colorimetric mercury ion sensor based on a rhodamine derivative and application. The colorimetric sensor is a novel colorimetric sensor prepared by taking rhodamine B, quadrol, Lawesson reagent and 4-chloro-7-nitrobenzofuran as raw materials. Mercury ions can be subjected to high-selectivity and high-sensitivity detection by the colorimetric sensor in a mixed solvent of acetonitrile and water. Compared with the existing mercury ion detection technology, the novel colorimetric sensor is simple in synthetic route, low in invested cost and simple in used detection device, is suitable for amplification synthesis and actual production application and has great application prospect in technical fields of analytical chemistry, environmental sciences, life sciences and the like.

The invention discloses a two / three-cluster glycosyl rhodamine derivative with general formulas I and II. Researches show that the obtained two / three-cluster glycosyl rhodamine derivative is good in water solubility, sensitive to pH and capable of being used as a fluorescent probe for monitoring pH changes so as to be directly used for monitoring pH changes in solutions, and has important scientific significance in research and application for monitoring on pH changes in cells and environments. Meanwhile, it is found that within the pH range between 4.35 and 7.5, the higher the acidity is, the higher the fluorescence intensity of the two types of compounds is, and the two / three-cluster glycosyl rhodamine derivative can be lightened in liver cancer cells (HepG2) under the slightly-acid condition to release strong red fluorescence and can be positioned in lysosome in a targeted manner. Meanwhile, the inventor established a preparation method of the corresponding compounds, the click chemistry reaction technology is used for the method, and synthesis is easy and scientific.