95results about "Acridine dyes" patented technology

The present invention describes new compositions of matter in the form of labeled nucleoside polyphosphates with four or more phosphates. In addition compositions of nucleoside polyphosphates with four or more phosphates that are substrates for nucleic acid polymerases with enhanced substrate properties and methods of using these nucleoside polyphosphates for nucleic acid detection, charcterization and quantification are described. The compositions provided by this invention include nucleoside polyphosphate, dideoxynucleoside polyphosphate, or deoxynucleoside polyphosphate analogues which have calorimetric, chemiluminescent, or fluorescent moieties, mass tags or an electrochemical tags attached to the terminal-phosphate. When a nucleic acid polymerase uses this analogue as a substrate, an enzyme-activatable label would be present on the inorganic polyphosphate by-product of phosphoryl transfer. Cleavage of the polyphosphate product of phosphoryl transfer via phosphatase leads to a detectable change in the label attached thereon. When the polymerase assay is performed in the presence of a phosphatase, there is provided a convenient method for real-time monitoring of DNA or RNA synthesis and detection of a target nucleic acid.

The present invention describes methods of detecting a nucleic acid in a sample, based on the use of terminal-phosphate-labeled nucleotides as substrates for nucleic acid polymerases. The methods provided by this invention utilize a nucleoside polyphosphate, dideoxynucleoside polyphosphate, or deoxynucleoside polyphosphate analogue which has a colorimetric dye, chemiluminescent, or fluorescent moiety, a mass tag or an electrochemical tag attached to the terminal-phosphate. When a nucleic acid polymerase uses this analogue as a substrate, an enzyme-activatable label would be present on the inorganic polyphosphate by-product of phosphoryl transfer. Cleavage of the polyphosphate product of phosphoryl transfer via phosphatase leads to a detectable change in the label attached thereon. When the polymerase assay is performed in the presence of a phosphatase, there is provided a convenient method for real-time monitoring of DNA or RNA synthesis and detection of a target nucleic acid.

The present invention relates to improved methods of detecting a target using a labeled substrate or substrate analog. The methods comprise reacting the substrate or substrate analog in an enzyme-catalyzed reaction which produces a labeled moiety with independently detectable signal only when such substrate or substrate analog reacts. The present invention, in particular, describes methods of detecting a nucleic acid in a sample, based on the use of terminal-phosphate-labeled nucleotides as substrates for nucleic acid polymerases. The methods provided by this invention utilize a nucleoside polyphosphate, dideoxynucleoside polyphosphate, or deoxynucleoside polyphosphate analogue which has a colorimetric dye, chemiluminescent, or fluorescent moiety, a mass tag or an electrochemical tag attached to the terminal-phosphate. When a nucleic acid polymerase uses this analogue as a substrate, an enzyme-activatable label would be present on the inorganic polyphosphate by-product of phosphoryl transfer. Cleavage of the polyphosphate product of phosphoryl transfer via phosphatase leads to a detectable change in the label attached thereon. When the polymerase assay is performed in the presence of a phosphatase, there is provided a convenient method for real-time monitoring of DNA or RNA synthesis and detection of a target nucleic acid.

The present invention describes methods of using terminal-phosphate-labeled nucleotides in the presence of a manganese salt to enhance their substrate properties towards various enzymes. Particularly described are methods of detecting a nucleic acid in a sample, based on the use of terminal-phosphate-labeled nucleotides as substrates for nucleic acid polymerases, in the presence of a manganese salt. Further provided are manganese complexes of terminal-phosphate-labeled nucleotides as well as terminal-phosphate-labeled nucleotides with new linkers with enhanced substrate properties.

The invention relates to a thermal activation delayed fluorescent material with a general formula of the structure shown as the formula (I) or the formula (II). D is one of phenoxazinyl, phenothizainyl, 9,9-dimethyl acridine, 9-methyl phenazinyl, 9-phenyl phenazinyl, 4-phenoxazinyl-1-phenyl, 4-phenothizainyl-1-phenyl, 4-(9,9-dimethyl)acridinyl-1-phenyl, 4-(9-methyl)-phenazinyl-1-phenyl, 4-(9-phenyl)phenazinyl-1-phenyl and 3,5-bis-carbazolyl-1-phenyl. The invention further relates to an organic electroluminescent device which comprises a light-emitting layer, and luminescent dye of the light-emitting layer is the thermal activation delayed fluorescent material. The singlet state-triplet state energy gap (delta EST) of the thermal activation delayed fluorescent material is very small, triplet state excitors can be converted into singlet state excitors through inverse intersystem crossing (RIST) to emit light, and the efficiency and stability of an OLED device can be improved. The formula is shown in the description.

The present invention describes indenocarbazole derivatives having electron and hole transport properties, especially for use in the emission layer and/or charge transport layer of electroluminescent devices or as matrix material. The invention further provides a process for preparing the compounds of the invention, and electronic devices comprising them.

Methods of using dyes and associated technology are provided. A dye, such as a monomeric dye or a dimeric dye, may be used in a nucleic acid gel staining application and/or a nucleic acid detection application. Such a dye and a salt that comprises an anion that is associated with a strong acid and a cation that is associated with a strong base may be used in such an application. A dimeric dye, such as a dimeric dye capable of forming a hairpin-like structure, may be used to stain and/or detect nucleic acids via a release-on-demand mechanism. A dimeric dye having low background fluorescence in the absence of nucleic acids and high fluorescence in the presence of nucleic acids, upon binding therewith, may be used to stain and/or detect nucleic acids.

Provided are a novel spiro compound, and an organic luminescence device using the spiro compound and having an optical output with an extremely high efficiency and a high luminance, and an extremely high durability. The Spiro compound is represented by the following general formula [I]:

(wherein R₁, R₂, R₃, and R₄ represent a hydrogen atom, a substituted or unsubstituted alkyl group, a substituted or unsubstituted aralkyl group, a substituted or unsubstituted aryl group, a substituted or unsubstituted heterocyclic group, a substituted amino group, a cyano group, or a halogen atom, and R₁, R₂, R₃, and R₄ may be identical or different from each other; and Ar₁ and Ar₂ represent a substituted or unsubstituted condensed polycyclic aromatic group or a substituted or unsubstituted condensed polycyclic heterocyclic group, which may be identical or different from each other.)

Reagents are provided for the introduction of phosphonate groups into fluorescent dyes. Methods are also provided for preparing dye conjugates.

Display comprising at least one organic light emitting diode, wherein the at least one organic light emitting diode comprises an anode, a cathode, a light emitting layer between the anode and the cathode, and at least one layer comprising a compound according to formula (I) between the cathode and the light emitting layer:

wherein A¹ and A² are independently selected from halogen, CN, substituted or unsubstituted C₁-C₂₀-alkyl or heteroalkyl, C₆-C₂₀-aryl or C₅-C₂₀-heteroaryl, C₁-C₂₀-alkoxy or C₆-C₂₀-aryloxy,

A³ is selected from substituted or unsubstituted C₆-C₄₀-aryl or C₅-C₄₀-heteroaryl,

m=0, 1 or 2,

n=0, 1 or 2.

A compound represented by Chemical Formula 1, and a photoelectric device, an image sensor, and an electronic device including the same are disclosed.

In Chemical Formula 1, each substituent is the same as defined in the detailed description.

The present invention provides simple, rapid methods and procedures for analyzing cells, hereunder quantitative and qualitative assessment of cells, such as viability. The present invention relates to the use of various optionally substituted reporter compounds particularly detectable upon their reaction with thiol-containing species present in higher concentrations in intact (e.g., living) cells than in non-intact (e.g., dead, stressed and apoptotic) cells. The present invention also relates to the use of various optionally substituted reporter compounds particularly detectable upon their reaction with species present in intact and/or non-intact cells. Moreover, the present invention relates to the use of measuring techniques and/or instruments coupled with the use of various optionally substituted reporter compounds. The invention further relates to compositions used in methods for analyzing cells, such as a composition comprising N-(7-dimethylamino-4-methyl-3-coumarinyl)-maleimide (DACM).

The present invention describes indenofluorene derivatives of the general formula I, II, III or IV having emitting and hole-transporting properties, in particular for use in the emission and/or charge-transport layer of electro-luminescent devices. The invention furthermore relates to a process for the preparation of the compounds according to the invention and to electronic devices comprising same.

The present invention relates to compounds according to formula (I), to the use thereof in organic electronic devices, and to organic electronic devices that contain compounds according to formula (I), preferably as hole transport materials and/or as matrix materials, in particular in combination with a further matrix material.

A hair-dyeing agent, which contains at least one compound represented by formula (1) or (2) as a dye:

wherein X₁ and X₂ each are an oxygen atom, nitrogen atom, sulfur atom, or dialkylmethylene group; R₁, R₂, R₃, R₄, R₅, R₆, R₇, and R₈ each independently are a hydrogen atom or substituent; M₁ and M₂ each are a counter ion to balance the charge of the molecule; and m₁ and m₂ each are a number of 0 or more necessary to neutralize the charge of the molecule.

Hydrophilic, chemiluminescent acridinium compounds containing zwitterions are disclosed. These acridinium compounds, when used as chemiluminescent labels in immunochemistry assays and the like, exhibit decreased non-specific binding to solid phases and provide increased assay sensitivity.

The present invention relates to novel organic electroluminescent compounds, and organic electroluminescent devices comprising the same. Specifically, the novel organic electroluminescent compounds according to the invention are characterized in that they are represented by Chemical Formula (1):

A compound represented by the following general formula is useful as a light emitting material. X represents an oxygen atom or a sulfur atom. R¹ to R⁸ represent a hydrogen atom or a substituent, provided that at least one of R¹ to R⁸ is a carbazolyl group, etc.