Hybridization probe for fluorescent detection of copper ions in water phase and preparation method thereof

A fluorescence detection, copper ion technology, applied in chemical instruments and methods, fluorescence/phosphorescence, measurement devices, etc., can solve problems such as poor water solubility, inability to detect copper traces in aqueous phase and intracellular fluorescence imaging, etc. The effect of few steps, high sensitivity and mild reaction conditions

Active Publication Date: 2016-11-09
SHANGHAI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] One of the objectives of the present invention overcomes the shortcomings of poor water solubility of existing organic fluorescent molecular probes, the inability to realize the trace detection of copper in the aqueous phase and the shortcomings of intracellular fluorescence imaging, and provides an inorganic matrix with mesoporous silica nanospheres, Hybrid probes for fluorescence detection of copper ions in aqueous phase using rhodamine derivatives as fluorescent detection groups

Method used

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  • Hybridization probe for fluorescent detection of copper ions in water phase and preparation method thereof
  • Hybridization probe for fluorescent detection of copper ions in water phase and preparation method thereof
  • Hybridization probe for fluorescent detection of copper ions in water phase and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Preparation and characterization of hybrid nanoprobe MSN@DFPP-RhB@PEG.

[0026] (1) Dissolve 0.05~0.50 g cetyltrimethylammonium bromide (CTAB) and 0.02~0.10 g triethanolamine (TEA) in 20 mL of pure water, and then heat to 95°C for reaction. After reacting for 1 to 2 hours, add 1.5 to 5 mL of ethyl orthosilicate to the reaction system, continue to react for 1 to 2 hours after the dropwise addition is complete, and centrifuge to wash to obtain a white solid. After that, the white solid was dissolved in ethanol by rapid ion exchange method, and then 0.1 g~1.0 g NH dissolved in it was added 4 NO 3 The ethanol solution is heated and reacted for 2~4 h, and finally the white solid is washed and dried to obtain mesoporous silica nanosphere MSN.

[0027] (2) Add the mesoporous silica nanospheres and 3-isocyanatopropyltriethoxysilane obtained in the above step (1) to the toluene solution in a mass ratio of 30:1-10:1, Heat to reflux for 12-24 h, centrifuge the obtained prod...

Embodiment 2

[0034] Example 2: Preparation of hybrid nanoprobe MSN@RH101@PEG.

[0035] (1) Dissolve 0.05~0.50 g cetyltrimethylammonium bromide (CTAB) and 0.02~0.10 g triethanolamine (TEA) in 20 mL of pure water, and then heat to 95°C for reaction. After reacting for 1 to 2 hours, add 1.5 to 5 mL of ethyl orthosilicate to the reaction system, continue to react for 1 to 2 hours after the dropwise addition is complete, and centrifuge to wash to obtain a white solid. After that, the white solid was dissolved in ethanol by rapid ion exchange method, and then 0.1 g~1.0 g NH dissolved in it was added 4 NO 3 The ethanol solution is heated and reacted for 2~4 h, and finally the white solid is washed and dried to obtain mesoporous silica nanosphere MSN.

[0036] (2) Add the mesoporous silica nanospheres and 3-isocyanatopropyltriethoxysilane obtained in the above step (1) to the toluene solution in a mass ratio of 30:1-10:1, Heat to reflux for 12-24 h, centrifuge the obtained product and wash it with eth...

Embodiment 3

[0041] Example 3: Detection of copper ion by hybrid nanoprobe MSN@DFPP-RhB@PEG.

[0042] (1) Weigh the hybrid fluorescent nanoprobe MSN@DFPP-RhB@PEG prepared in Example 1, and make it into a 1.0 mg / mL aqueous solution.

[0043] (2) Prepare concentrated solutions (0.1M) of various metal ions, including Na + , K + , Mg 2+ , Ca 2+ , Ba 2+ , Zn 2+ , Li + , Mn 2 + , Co 2+ , Hg 2+ Wait, and the ion to be detected Cu 2+ .

[0044] (3) In the selectivity experiment, a certain equivalent of various metal ions were added to 2.0 mL of the hybrid fluorescent nanoprobe aqueous solution, and then the absorption spectrum and fluorescence spectrum were tested. The results are shown in the attachment respectively. Figure 4 And Figure 5 .

[0045] (4) In the titration experiment, use a pipette to draw 2.0 mL of the hybrid fluorescent nanoprobe aqueous solution and place it in a 1.0 cm×1.0 cm quartz cuvette, and use a micro-injector to sequentially add Cu to the solution 2+ The solution is tested for u...

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Abstract

The invention relates to a hybridization probe for fluorescent detection of copper ions in a water phase and a preparation method thereof. The hybridization probe is prepared by grafting a fluorescence detection group, i.e., a rhodamine derivative, into a mesoporous silica nanosphere inorganic matrix via a covalent bond and carrying out surface polyethylene glycol (PEG) modification, and can realize fluorescent detection of copper ions in the water phase. The preparation method for the hybridization fluorescent nanometer probe is mild in reaction conditions and simple in synthesis steps. The hybridization fluorescent nanometer probe is applicable to detection of copper ions in water, has good anti-interference capability, good stability and high selectivity, can realize detection of a single copper ion, and is free of influence by co-existing ions, and fluorescence and color changes of the probe are visible to naked eyes. Moreover, the hybridization fluorescent nanometer probe is applicable to fluorescence imaging of Cu<2+> in living cells, and has potential application value in fluorescent detection of Cu<2+> in a living body and in early diagnosis and pathogenesis research of diseases related to Cu<2+>.

Description

Technical field [0001] The invention relates to a hybrid probe for fluorescence detection of copper ions in an aqueous phase and a preparation method thereof. Background technique [0002] Copper is an essential trace element in the human body and an important part of the protein and enzymes in the body. Many important enzymes in the body require the participation and activation of trace copper. For example, copper can catalyze the synthesis of hemoglobin. Lack of copper in the human body can cause anemia, abnormal hair, abnormal bones and arteries, and even brain disorders. Studies have shown that copper deficiency can increase plasma cholesterol and increase the risk of atherosclerosis, which is an important factor in causing coronary heart disease. Severe copper deficiency and long-term marginal copper deficiency can also cause dysplasia in children and some endemic diseases. However, excessive copper intake can also bring undesirable consequences. This is because copper ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/06G01N21/64
CPCC09K11/06C09K2211/1029C09K2211/1088G01N21/6486
Inventor 施利毅刘金亮孟宪福孙丽宁徐艳霞
Owner SHANGHAI UNIV
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