Up-conversion fluorescent probe rhodamine derivative and application thereof

A technology of fluorescent probes and derivatives, applied in the field of up-conversion fluorescence enhancement reagents, to achieve the effects of less damage to biological cells, high yield, and less damage to cells

Active Publication Date: 2019-09-13
SUZHOU UNIV OF SCI & TECH
View PDF11 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, detection methods using anti-Stokes fluorescence (i.e., upconversion fluorescence) are rarely reported

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Up-conversion fluorescent probe rhodamine derivative and application thereof
  • Up-conversion fluorescent probe rhodamine derivative and application thereof
  • Up-conversion fluorescent probe rhodamine derivative and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Add Rhodamine B (2 g, 4.2 mmoL), ethylenediamine (1 mL, 21 mmoL) and 50 mL of absolute ethanol into a 250 mL three-neck flask, and dissolve them by ultrasonication; heat to reflux at 80 ° C, and track the reaction process with a plate , the developer is methanol:dichloromethane=10:1, the reaction is carried out for 24h, stop the reaction, and cool to room temperature; the ethanol is removed by distillation under reduced pressure to obtain a brown solid mixture, and the product is purified by column chromatography. The developer is methanol / dichloromethane Chloromethane (10 / 1, v / v). 1.5 g (yield: 70%) of the brown intermediate rhodamine B-ethylenediamine was obtained. 1 H NMR (400 MHz, CDCl 3 ) δ 7.91 (dd, J = 5.6, 3.1 Hz,1H), 7.46 (dt, J = 7.2, 3.7 Hz, 2H), 7.10 (dd, J = 5.6, 3.0 Hz, 1H), 6.51-6.24 (m, 6H), 3.35 (q, J = 7.2 Hz, 8H), 3.21 (t, J = 6.5 Hz, 2H), 2.47 (t, J = 6.5 Hz, 2H), 1.18 (t, J = 7.0 Hz, 12H) (see attached figure 1 ). MS, Calculated: [M ...

Embodiment 2

[0041] The probe molecule RhB-NA was added to ethanol to prepare a RhB-NA ethanol mother solution with a concentration of 1 mM.

[0042] Add 3 mL of ethanol / buffer solution (buffer solution is Na 2 HPO 4 / NaH 2 PO 4 aqueous solution, pH=7, 1 / 2, v / v), and then take 30 μL of RhB-NA ethanol mother solution (concentration: 1mM) into the above-mentioned quartz cuvette, ultrasonically dissolve it, and then prepare a 10 μM Probe RhB-NA solution.

[0043] Similarly, add 3 mL ethanol / buffer solution (Na 2 HPO 4 / NaH 2 PO 4 aqueous solution, pH=7, 1 / 2, v / v), and then take 300 μL RhB-NA ethanol mother solution (concentration: 1mM) into the above-mentioned quartz cuvette, ultrasonically dissolve it, and then prepare 100 μM Probe RhB-NA solution.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
wavelengthaaaaaaaaaa
wavelengthaaaaaaaaaa
Login to view more

Abstract

The invention discloses an up-conversion fluorescent probe rhodamine derivative and an application thereof. The preparation method comprises the following steps: performing a reaction on rhodamine B and ethylenediamine in a nitrogen gas atmosphere and an organic solvent to obtain rhodamine-ethylenediamine; and performing a reaction on the rhodamine-ethylenediamine and phenyl isothiocyanate to obtain the up-convertion fluorescent probe rhodamine derivative. The up-conversion fluorescent probe rhodamine derivative provided by the invention has the high-efficiency down-conversion fluorescence-enhanced response recognition characteristics for mercury ions and has the high-efficiency up-conversion fluorescence-enhanced response recognition characteristics for the mercury ions when used as a fluorescent probe.

Description

technical field [0001] The invention belongs to the field of upconversion fluorescence enhancement reagents and the technical field of heavy metal ion upconversion fluorescence probes, and specifically relates to a rhodamine derivative probe with upconversion fluorescence enhancement response and a preparation method thereof, and the use of the probe in detecting water environment Or the application of mercury ions in living organisms. Background technique [0002] Mercury ions are extremely harmful to organisms and the natural environment, and it is of great significance to make rapid and high-sensitivity detection of mercury ions. At present, many methods for the determination of mercury ions have been reported, mainly including: spectrophotometry, fluorescence analysis, electrochemical method and atomic absorption spectrometry. Among them, the fluorescence analysis method has attracted extensive attention due to its advantages of high sensitivity, specific selectivity, s...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C07D491/107C09K11/06G01N21/64A61K49/00
CPCA61K49/0041C07D491/107C09K11/06C09K2211/1011C09K2211/1029C09K2211/1088G01N21/6428G01N21/643
Inventor 王筱梅宋飒飒夏方媛朱琳叶常青于雪王伟飒
Owner SUZHOU UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap