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Application of rhodamine B derivative as cellular mitochondrion fluorescence coloring agent

A derivative and mitochondrial technology, which is applied in the field of cell mitochondrial fluorescent dyes, can solve the problems of short retention period and loss of probes, and achieve the effect of long retention time, high photostability and high stability

Inactive Publication Date: 2012-06-27
XIAMEN UNIV
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

Some mitochondrial probes, such as rhodamine 123, have a short retention period in cells, and the intracellular probes are easily lost over time

Method used

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  • Application of rhodamine B derivative as cellular mitochondrion fluorescence coloring agent
  • Application of rhodamine B derivative as cellular mitochondrion fluorescence coloring agent
  • Application of rhodamine B derivative as cellular mitochondrion fluorescence coloring agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0024] 1) Rhodamine B (g), triphenylphosphine (g), hexachloroethane (g), tert-butoxypiperazine (g) were sequentially added to a round bottom containing 25ml triethylamine and 100ml dichloromethane In the flask, the electromagnetic stirring system was used. After reacting at room temperature for 12 hours, the initial product was separated and purified by silica gel column, and the initial product was separated and purified by silica gel column after being treated with trifluoroacetic acid. React the product with chloroacetic anhydride at room temperature for 12 hours, then separate and purify again to obtain Rhodamine B-methylchlorocarbonylpiperazine;

[0025] 2) Rhodamine B-methylchlorocarbonylpiperazine (1mg / ml) was dissolved in methanol, and the excitation and emission fluorescence spectra were detected. According to the attached figure 1 The excitation and emission fluorescence spectrograms of Rhodamine B-methylchlorocarbonylpiperazine shown can be found to have a maximum ...

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Abstract

The invention discloses an application of a rhodamine B derivative containing methyl chloride carbonyl piperazine as a cellular mitochondrion fluorescence coloring agent. The rhodamine B derivative can be used as the cellular mitochondrion fluorescence coloring agent for high-selectivity long-term stability label of the cellular mitochondrion. The rhodamine B derivative enters the cellular mitochondrion after incubating with different cells. The rhodamine B derivative has long retention time in the cellular mitochondrion, and has high stability, is not easily optically quenched, and does not react with active substance in the cells. The rhodamine B derivative can be used for specifically and stably labeling different cellular mitochondria. The rhodamine B derivative can be used as a fluorescent label substance for different cells, and can be used for long-time labeling and tracking the mitochondria in the cells.

Description

Technical field [0001] The invention involves the use of Landmine B derivatives with methyl azine, and the use of Landming B derivatives with methyl chloropatrazine as a cell mitochondrial fluorescent chromaton. Background technique [0002] Mitochondria is a cytoter in the true nuclear cells, and its internal environment is weakly alkaline.Mitochondria can not only provide energy for cells, but also have a direct connection with the development of cells to devour, apoptosis, immune defense, and cancer development (POOT, M., Y. Z. Z. zhang, et al. Analysis of Mitochondric Morphology and Function with Normal Fixable FluoreScitentitentStains. Journal of history & cytochemistry.1999; 44 (12): 1363-1372.).Therefore, the molecular probe that can marked and track the mitochondria in the living cells is of great significance to study the biological function of mitochondria and the diagnosis of living cancer cells (Ishiguro, K., T. Ando, et al. Novel Application of4-nitro-7- (1-Piperazin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/06C09B57/00G01N33/52
CPCC09B11/24G01N33/52
Inventor 韩家淮吴淑琪韩守法
Owner XIAMEN UNIV
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