Micro-fluid control electrochemical biological sensing system for simultaneous detection on different serum markers of prostate cancer

A serum marker and biosensing technology, applied in the field of microfluidic electrochemical biosensing system, can solve the problems of inability to simultaneously detect multiple markers, complicated steps for prostate cancer disease, and large size of instruments and equipment, and achieves Ease of integration, low sample consumption, and high sensitivity

Active Publication Date: 2014-03-05
SHANGHAI INST OF APPLIED PHYSICS - CHINESE ACAD OF SCI
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0010] The purpose of the present invention is to provide a microfluidic electrochemical biosensing system for simultaneous detection of different types of serum markers of prostate cancer, so as to solve the cumbersome steps of the prostate cancer disease detection technology in the prior art, and the large volume of equipment and equipment. Expensive, low specificity and inability to detect multiple markers at the same time

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  • Micro-fluid control electrochemical biological sensing system for simultaneous detection on different serum markers of prostate cancer
  • Micro-fluid control electrochemical biological sensing system for simultaneous detection on different serum markers of prostate cancer
  • Micro-fluid control electrochemical biological sensing system for simultaneous detection on different serum markers of prostate cancer

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Embodiment 1

[0051] This embodiment adopts such as figure 1 The shown microfluidic electrochemical biosensing system is used for simultaneous rapid detection of multiple protein markers of prostate cancer disease. The steps are as follows. First, 10-50 μg / mL of PSA-Ab, PSMA-Ab, and IL-6-Ab are respectively fixed on the surface of the electrode array to form an array electrochemical sensing interface. Continuously draw a mixture of avidin-HRP, water, buffer (0.01M phosphate, 0.14M NaCl, 2.7mM KCl, pH7.2), biotin-PSA, biotin-PSMA and biotin-IL-6 manually through a 1mL syringe. Concentration of PSA, PSMA and IL-6 antigen mixture solution, there are 0.5-1cm long air bubbles between each functional solution zone, and the volume of each solution zone is 1-20μL; then the prepared chip sensor, The continuous sampling unit and the vacuum system are connected as a whole, and the suction flow rate of the syringe pump is adjusted to 10-20 μL / min. When the solution zone in the delivery unit starts to ...

Embodiment 2

[0053] This embodiment adopts such as figure 1The shown microfluidic electrochemical biosensing system was used for the simultaneous rapid detection of different types of tumor markers including proteins and miRNAs in prostate cancer disease. The steps are as follows, first immobilize 10-50 μg / mL PF-4-Ab and 0-2 μM DNA tetrahedral nanostructure probes on the surface of the electrode array (electrodes for assembling nucleic acid probes need to be directly deposited on the electrode array by electrochemical redox method) Deposit a layer of nano-gold on the surface of the electrode, or directly print the nano-gold layer) to form an array electrochemical sensing interface. Then avidin-HRP, water, buffer (0.01M phosphate, 0.14M NaCl, 2.7mM KCl, pH7.2)), biotin-modified secondary antibody biotin-PF-4 and miR21 The mixture of partially complementary signal probe chain biotin-miR21, and the mixture solution zone of a certain concentration of PF-4 and miR21, there are 0.5-1cm long air...

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Abstract

The invention provides a micro-fluid control electrochemical biological sensing system for simultaneous detection on different serum markers of prostate cancer. The system comprises a continuous feeding unit, a micro-fluid control chip and a power system, wherein the continuous feeding unit is used for sequentially conveying a sample solution, a sample eluant, a signal probe solution, a signal probe eluant and an electrochemical detection buffer solution; the sample solution contains a protein marker and/or a miRNA marker for the prostate cancer; the micro-fluid control chip consists of one or more micro-channel networks; the micro-fluid control chip covers an electrode array so as to form a channel system; antibodies and/or capture probes which have interaction with the sample solution are fixed on the upper surface of the electrode array; the channel system is connected with the continuous feeding unit; the power system is used for providing power for the continuous feeding unit. The invention innovatively provides the micro-fluid control electrochemical biological sensing system which is capable of simultaneously detecting different serum markers closely related to prostate cancer diseases and is high in sensitivity and low in cost.

Description

technical field [0001] The invention relates to the field of microfluidic electrochemical biosensing systems, in particular to a microfluidic electrochemical biosensing system for simultaneous detection of different types of serum markers of prostate cancer. Background technique [0002] Prostate cancer (prostate cancer) is the most common malignant tumor of the male reproductive system, and has become the most common tumor in men in European and American countries. Prostate cancer is an age-related disease. With the increase in the average life expectancy of the Chinese population, prostate cancer has become a huge threat to elderly men in my country. Prostate-specific antigen (PSA) as a diagnostic marker of prostate cancer is by far the most effective clinical indicator for early diagnosis of prostate cancer. However, the specificity of PSA is not good enough, and some benign lesions of the prostate can also increase it. This false positive problem usually leads to unnec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/416
Inventor 樊春海杨帆左小磊黄庆
Owner SHANGHAI INST OF APPLIED PHYSICS - CHINESE ACAD OF SCI
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