Preparation method and application of sensor for simultaneous detection of multiple aflatoxins

A kind of technology of aflatoxin and sensor, which is applied in the field of preparation of simultaneous detection of multiple aflatoxin sensors, can solve the problems of simultaneous detection of multiple aflatoxins, difficulty in fast analysis, cumbersome detection process, etc., and achieve easy integration The effect of simplification, low cost and simple detection method

Active Publication Date: 2013-07-10
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0010] (1) The testing process is cumbersome, time-consuming and labor-intensive;
[0011] (2) The equipment is expensive and the operation is complicated, so it is difficult to achieve rapid analysis;
[0012] (3) The sensitivity is poor, and the simultaneous detection of multiple aflatoxins cannot be realized

Method used

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  • Preparation method and application of sensor for simultaneous detection of multiple aflatoxins
  • Preparation method and application of sensor for simultaneous detection of multiple aflatoxins

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Embodiment 1 A kind of preparation method that simultaneously detects multiple aflatoxin sensors described in the present invention, combines figure 1 ,Proceed as follows.

[0035] On the surface of a rectangular substrate ① coated with a layer of polyester film, 1 reference electrode ② is printed with silver paste, 5 working electrodes ③, 1 counter electrode ④ and 7 electrodes are printed with conductive carbon paste. wire ⑤ and the end of the lead wire ⑥, and on all electrodes and substrates other than the end of the lead wire, an insulating layer is printed with insulating paste, and a circular electrolytic cell groove ⑦ is formed in the electrode area, and a Five-channel screen-printed electrodes.

Embodiment 2

[0036] Example 2 A preparation method for simultaneously detecting multiple aflatoxin sensors according to the present invention, combining figure 1 ,Proceed as follows.

[0037] (1) On 5 working electrodes of the multi-channel printed electrode prepared in Example 1, drop-coat 5 μ L of silver nanoparticles-nitrogen-doped graphene-hydroxypropyl chitosan conductive composite nanomaterial; the conductive composite nanomaterial The mass ratio of silver nanoparticles to nitrogen-doped graphene is 1 : 8. Ultrasonic mixing for 30 minutes, plus ultrasonic dispersion of hydroxypropyl chitosan solution for 30 minutes, to make a silver nanoparticle-nitrogen-doped graphene-hydroxypropyl chitosan conductive composite nanomaterial with a mass ratio of 1:8:1 solution;

[0038] (2) On the 5 working electrodes prepared in step (1), add 5 μL of antibodies to 4 kinds of aflatoxins and total aflatoxins at a concentration of 10 μg / mL respectively, and then add 10 μL of 1% aflatoxin Bovine ser...

Embodiment 3

[0039] Example 3 Change the amount of drop-coated silver nanoparticles-nitrogen-doped graphene-hydroxypropyl chitosan conductive composite nanomaterial solution to 10 μL, the mass ratio is 2: 10: 5, and the others are the same as in Example 2.

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Abstract

The present invention discloses a preparation method and application of a printing electrode electrochemical immunosensor for simultaneous detection of multiple aflatoxins, and belongs to technical fields of biological sensing, nano-functional materials and food safety analysis. The surface of a rectangular substrate coated with a polyester film is printed with seven electrodes, including five carbon working electrodes, a silver reference electrode and a counter electrode, and with seven electrode lead lines and electrode lead line ends. The present invention is characterized in that: a silver nanoparticles-nitrogen-doped graphene-hydroxypropyl chitosan conductive composite nanomaterial is used for fixing aflatoxin B1, aflatoxin B2, aflatoxin G1, aflatoxin G2, and total aflatoxin antibody to the surfaces of each working electrode, a detection liquid is dripped and coated on the working electrodes and the working electrodes are connected with a detection circuit of a multi-channel electrochemical workstation, thus the simultaneous detection of five aflatoxins are achieved. The sensor is simple in preparation, easy for processing, low in cost, and easy to carry. The detection method is simple, rapid, high in sensitivity and good in specificity.

Description

technical field [0001] The invention belongs to the technical field of biosensing, nanometer functional materials and food safety analysis, and in particular relates to a preparation method and application of a sensor for simultaneously detecting multiple aflatoxins. Background technique [0002] Aflatoxins are metabolites of Aspergillus flavus and Aspergillus parasiticus. The aflatoxins produced mainly include aflatoxin B1, aflatoxin B2, aflatoxin G1 and aflatoxin G2 (referred to as four aflatoxins), which are ubiquitous. In mildewed food and food products, it can contaminate food and feed through various channels, directly or indirectly enter the human food chain, and threaten human health and life safety. [0003] Aflatoxin is the most toxic mycotoxin found so far. Its toxicity is equivalent to 10 times that of potassium cyanide and 68 times that of arsenic. Long-term intake of aflatoxin can induce liver cancer. N-nitrosamines are 75 times larger and are currently recogn...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48
Inventor 杜斌魏琴黎荣霞于海琴张勇吴丹李贺马洪敏
Owner UNIV OF JINAN
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