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Preparation method of label-free electrochemical immunosensor for nonyl phenol detection

An immunosensor, electrochemical technology, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., can solve the problems of cumbersome process and low yield, achieve simple preparation process, convenient operation, and improve the strength of electrochemical response signal Effect

Active Publication Date: 2015-12-16
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, most of the synthesis methods are synthesized separately, and then compound the catalyst and the carrier, the process is cumbersome and the yield is not high

Method used

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  • Preparation method of label-free electrochemical immunosensor for nonyl phenol detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 MoS 2 / Preparation method of AuPd

[0027] Take 35mL of CTAB solution with a concentration of 0.1mol / L, add 2mL of HAuCl with a concentration of 0.06mol / L 4 solution and 2 mL of H at a concentration of 0.03 mol / L 2 PdCl 4 solution, after stirring for 15 minutes, continue to stir and add 2 mL of ascorbic acid solution with a concentration of 0.3 mol / L, 0.5 mL of Na with a concentration of 0.1 mol / L 2 MoO 4 solution and 0.01 g of Na 2 S, after stirring for 15 minutes, put it into the reaction kettle, and react for 14 hours at 180°C; after cooling to room temperature, use deionized water to wash centrifugally, and vacuum dry at 40°C to obtain MoS 2 / AuPd, which was dissolved in deionized water to obtain MoS at a concentration of 1 mg / mL 2 / AuPd solution.

Embodiment 2

[0028] Example 2 MoS 2 / Preparation method of AuPd

[0029] Take 35mL of CTAB solution with a concentration of 0.1mol / L, add 4mL of HAuCl with a concentration of 0.06mol / L 4 solution and 4 mL of H at a concentration of 0.03 mol / L 2 PdCl 4 solution, after stirring for 15 minutes, continue to stir and add 4 mL of ascorbic acid solution with a concentration of 0.3 mol / L, 1.0 mL of Na with a concentration of 0.1 mol / L 2 MoO 4 solution and 0.02g Na 2 S, after stirring for 15 minutes, put it into the reaction kettle, and react for 16 hours at 150°C; after cooling to room temperature, use deionized water to wash centrifugally, and vacuum dry at 40°C to obtain MoS2 / AuPd, which was dissolved in deionized water to obtain MoS at a concentration of 1 mg / mL 2 / AuPd solution.

Embodiment 3

[0030] Example 3 MoS 2 / Preparation method of AuPd

[0031] Take 35mL of CTAB solution with a concentration of 0.1mol / L, add 6mL of HAuCl with a concentration of 0.06mol / L 4 solution and 6 mL of H at a concentration of 0.03 mol / L 2 PdCl 4 solution, after stirring for 15 minutes, continue to stir and add 6 mL of ascorbic acid solution with a concentration of 0.3 mol / L, 1.5 mL of Na with a concentration of 0.1 mol / L 2 MoO 4 solution and 0.03g Na 2 S, after stirring for 15 minutes, put it into the reaction kettle, and react for 12 hours at 220°C; after cooling to room temperature, use deionized water to wash centrifugally, and vacuum dry at 40°C to obtain MoS 2 / AuPd, which was dissolved in deionized water to obtain MoS at a concentration of 1 mg / mL 2 / AuPd solution.

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Abstract

The present invention discloses a preparation method of a label-free electrochemical immunosensor for nonyl phenol detection, and belongs to the technical field of novel nanometer function materials and environment detection. According to the present invention, the molybdenum disulfide / gold-palladium alloy nanometer composite material MoS2 / AuPd is prepared by using the one-pot method, and then the simple, rapid and sensitive label-free electrochemical immunosensor for nonyl phenol detection in real samples is prepared through the excellent adsorption performance and the excellent electrochemical catalytic performance of the molybdenum disulfide / gold-palladium alloy nanometer composite material MoS2 / AuPd.

Description

technical field [0001] The invention relates to a preparation method of a label-free electrochemical immunosensor based on inorganic nanocomposite materials, and the prepared sensor can be used for the detection of nonylphenol. The invention belongs to the technical field of new nano functional materials and environmental detection. Background technique [0002] Nonylphenol is an important fine chemical raw material and intermediate, and is widely used in the production of textile printing and dyeing auxiliaries, lubricating oil additives, pesticide emulsifiers, resin modifiers, resin and rubber stabilizers and other fields. However, due to its endocrine disrupting effect, it can promote the proliferation of breast cancer cells, and it is an organic pollutant, a carcinogen that interferes with endocrine, and is known as a "sperm killer". According to environmental standards and the recommended standards of the US Environmental Protection Agency, the content of nonylphenol i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N33/531G01N27/26
CPCG01N27/26G01N33/5306G01N33/531
Inventor 李娇娇魏琴张勇任祥马洪敏庞雪辉闫涛胡丽华范大伟
Owner UNIV OF JINAN
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