Thiosalicylic acid imidazoline, NiCl2. 6H2O and CuCl2. 2H2O are respectively subjected to
reflux stirring for 24 hours in an absolute
methanol solvent to prepare a complex with the following
structural formula shown in the following chemical formulas I-III: a synthesis method of a complex I-III
crystal comprises the following steps: respectively weighing 2 mmol and 1 mmol of
thiosalicylic acid, putting into a 100 mL round-bottom flask, adding 10 mL of DMF (
Dimethyl Formamide) and 20 mL of absolute
methanol to completely dissolve the
thiosalicylic acid, then adding the absolute
methanol into the round-bottom flask, and stirring for 24 hours to obtain the complex I-III
crystal. 1 mmol of imidazoline and 1 mmol of
metal salts including 2 mol of NiCl2. 6H2O, 2 mmol of CuCl2. 2H2O and 1 mmol of
anhydrous zinc chloride are sequentially added, a
reflux reaction is performed at 90 DEG C for 48 h, hot
filtration is performed, the obtained filtrate is placed in a
beaker and sealed with a sealing film, some small holes are punched, standing is performed at the
room temperature, and green complex crystals I and II and brown complex crystals III appear in the filtrate after the
solvent is naturally volatilized for five days; according to the application of the complexes I-III, the
crystal complexes I-III show certain inhibition activity in anti-
cancer cell tests such as
lung cancer A-549, and the anti-
cancer inhibition ratios of the crystal complexes I-III are 29.76%, 49.9% and 13.68% respectively.