Surface-enhanced Raman scattering aptamer sensor for detecting polychlorinated biphenyls and application

A Raman scattering nucleic acid and aptamer sensor technology, applied in Raman scattering, instruments, biological testing, etc., can solve the problems of complex sample pretreatment and low sensitivity, and achieve easy artificial synthesis and modification, high sensitivity, highly selective effect

Active Publication Date: 2018-11-06
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a surface-enhanced Raman scattering nucleic acid aptamer sensing analysis method for the problems of low sensitivity, time-consuming analysis, and complicated sample pretreatment of the existing PCB detection technology.

Method used

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  • Surface-enhanced Raman scattering aptamer sensor for detecting polychlorinated biphenyls and application
  • Surface-enhanced Raman scattering aptamer sensor for detecting polychlorinated biphenyls and application

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Embodiment 1

[0031] A surface-enhanced Raman scattering nucleic acid aptamer sensing and analysis method for PCB detection, the sensor is constructed through the following steps:

[0032] (1) All glass instruments used were soaked in aqua regia, cleaned with high-purity water, and dried for later use. 100 mL 0.01% (w / w) HAuCl 4 Bring to a boil, then add 1.5 mL of 1% (w / w) sodium citrate. Stir at 100°C for 15-20min until the solution turns wine red, that is, Au NPs are synthesized. Then add 800 μL of AgNO 3The solution (0.5%, w / w) was added to the boiling 100mL AuNPs solution, and 1mL of 1% (w / w) sodium citrate solution was added dropwise under stirring, then the solution was kept boiling for 1h, the heating was turned off, and at room temperature cool down.

[0033] (2) 10 mM 4-MBA was added to Au@Ag CS NPs to make the final concentration of 4-MBA 5 μM, and then reacted for a period of time. The nucleic acid aptamer solution with a concentration of 6 μM and the PCB72 solution with a c...

Embodiment 2

[0035] In order to ensure that this sensing analysis method exhibits the optimal SERS effect, the concentration of 4-MBA, the concentration of nucleic acid aptamer and the reaction time need to be optimized. The specific steps are: take 4-MBA with a concentration range of 1 μM-8 μM for Raman intensity test to obtain the optimal 4-MBA concentration; then change the concentration of nucleic acid aptamer, and consider the nucleic acid aptamer within the concentration range of 1 μM-10 μM The optimal concentration; Finally, consider the influence of different reaction times on the Raman intensity. The results showed that the optimal concentration of 4-MBA was 5 μM, the optimal concentration of nucleic acid aptamer was 6 μM, and the optimal reaction time was 25 min.

Embodiment 3

[0037] A good sensing analysis method also needs to ensure the reproducibility of the signal and the stability of the substrate. The specific experimental steps are as follows: prepare 5 batches of mixed solutions for Raman detection, select 10 points for each batch, and the relative standard deviation is 8.82%. The experimental results show that this sensing analysis method has good signal reproducibility and can ensure The reliability of the method; the experiment also conducted a Raman test on the same batch of substrates every 10 days, and the results proved that the substrates had similar Raman effects within 60 days, showing the stability of the substrates.

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Abstract

The invention relates to a surface-enhanced Raman scattering aptamer sensor for detecting polychlorinated biphenyls and application. Gold-silver core-shell nanoparticles are used as a Raman substrate;4-thiosalicylic acid as a Raman signal molecule is modified on the surface of the gold-silver core-shell nanoparticles; a PCB72 aptamer is used as a pollutant identification element for constructingto obtain the sensor. Compared with the prior art, the surface-enhanced Raman scattering aptamer sensor disclosed by the invention has the advantages that by adopting an advanced surface-enhanced Raman technology, the detection sensitivity of the sensor to PCB72 is greatly improved, the linear detection range of the sensor is 1pg/mL-1000pg/mL and the detection limit can reach 0.3pg/mL; meanwhile,by adopting the aptamer, specific recognition of the PCB72 is realized, and the detection selectivity of the sensor to the PCB72 is greatly improved. The sensor disclosed by the invention can be usedfor detection analysis of an actual water sample; compared with other analysis methods, an analysis method disclosed by the invention has the advantages that a sample does not need complex pretreatment; simplicity, quickness and ultrahigh sensitivity are realized.

Description

technical field [0001] The invention belongs to the field of water pollution detection and treatment, and in particular relates to a surface-enhanced Raman scattering nucleic acid aptamer sensor for detecting polychlorinated biphenyls and its application. Background technique [0002] Polychlorinated biphenyls (PCBs) are a class of persistent organic pollutants worldwide. Because they are extremely difficult to decompose, extremely insoluble in water, and easily soluble in fat and organic solvents, they can be abundant in human and animal fats. They can destroy the estrogen / androgen secretion system, thyroid hormone secretion system and other endocrine systems by interfering with the synthesis, transport, storage, metabolism and feedback regulation of hormones, which will affect the sex and weight of newborns, and seriously damage human health and life safety. In the 1970s, the production and use of polychlorinated biphenyls were banned in most countries because of their pe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65C12N15/115G01N33/53
CPCC12N15/115G01N21/658G01N33/5308
Inventor 曹同成陈倩倩刘斯瑶史慧杰赵国华
Owner TONGJI UNIV
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