Preparation method and application of combined targeted medicine controlled release system with gold nanometer cage being carrier

A technology of gold nanocage and drug controlled release, which is applied in the field of medicine and can solve the problems of poor treatment effect and large side effects

Inactive Publication Date: 2015-11-11
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the above situation, in order to overcome the defects of the prior art, the purpose of the present invention is to provide a preparation method and application of a compound targeted drug controlled release system using gold nanocages as a carrier, which can effectively solve the problem of the large side effects of existing tumor treatment drugs. , Poor therapeutic effect, Medication problems of tumor photothermal therapy

Method used

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  • Preparation method and application of combined targeted medicine controlled release system with gold nanometer cage being carrier

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] In concrete implementation, the present invention can be realized by the following steps:

[0023] 1) Preparation of gold nanocages wrapped with manganese zinc ferrite:

[0024] At room temperature, with stirring at 1000rpm, the HNO 3 Add Re to 2 o 3 , Fe(NO 3 ) 3 , Mn(NO 3 ) 2 , Zn(NO 3 ) 2 , then add citric acid and ethylene glycol under magnetic stirring, the molar ratio is HNO 3 :Re 2 o 3 : Fe(NO 3 ) 3 :Mn(NO 3 ) 2 : Zn(NO 3 ) 2 : citric acid: ethylene glycol = 1: 0.07: 0.5: 0.5: 1.86: 1: 1, then according to HNO 3 Add gold nanocages with a molar ratio of 1000:1, adjust the pH to 6 with ammonium bicarbonate, sonicate the probe for 45min, add ultrapure water to 15mL, centrifuge at 15000rpm for 5min, discard the supernatant, add ultrapure water to the precipitate to 15mL, centrifuge at 15000rpm for 5min, discard the supernatant, and obtain the precipitate; then add ultrapure water to the precipitate to reach 15mL, repeat centrifugation for 3-5 times ...

Embodiment 2

[0030] In specific implementation, the present invention can also be realized by the following steps:

[0031] 1) Preparation of gold nanocages wrapped with manganese zinc ferrite:

[0032] Under stirring at room temperature and a rotation speed of 1500rpm, the HNO 3 Add Re to 2 o 3 , Fe(NO 3 ) 3 , Mn(NO 3 ) 2 , Zn(NO 3 ) 2 , then add citric acid and ethylene glycol under magnetic stirring, the molar ratio is HNO 3 : Re 2 o 3 : Fe(NO 3 ) 3 :Mn(NO 3 ) 2 : Zn(NO 3 ) 2 : citric acid: ethylene glycol = 1: 0.07: 0.5: 0.5: 1.86: 1: 1, then according to HNO 3 Add gold nanocages with a molar ratio of 1000:1, adjust the pH to 6 with ammonium bicarbonate, sonicate the probe for 30 minutes, add ultrapure water to 10 mL, centrifuge at 15000 rpm for 5 minutes, discard the supernatant, add ultrapure water to the precipitate to 10mL, centrifuge at 15000rpm for 5min, discard the supernatant, and obtain the precipitate; add ultrapure water to the precipitate to 10mL, repeat cen...

Embodiment 3

[0038] The present invention can also be realized by the following steps in concrete implementation:

[0039] 1) Preparation of gold nanocages wrapped with manganese zinc ferrite:

[0040] At room temperature, with stirring at 2000rpm, the HNO 3 Add Re to 2 o 3 , Fe(NO 3 ) 3 , Mn(NO 3 ) 2 , Zn(NO 3 ) 2 , then add citric acid and ethylene glycol under magnetic stirring, the molar ratio is HNO 3 :Re 2 o 3 : Fe(NO 3 ) 3 :Mn(NO 3 ) 2 : Zn(NO 3 ) 2 : citric acid: ethylene glycol = 1: 0.07: 0.5: 0.5: 1.86: 1: 1, then according to HNO 3 Add gold nanocages with a molar ratio of 1000:1, adjust the pH to 6 with ammonium bicarbonate, sonicate the probe for 30 minutes, add ultrapure water to 25 mL, centrifuge at 15000 rpm for 5 minutes, discard the supernatant, and add ultrapure water to the precipitate to 25mL, centrifuge at 15000rpm for 5min, discard the supernatant, and obtain the precipitate; add ultrapure water to the precipitate to 25mL, repeat centrifugation for 3...

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Abstract

The invention relates to a preparation method and application of a combined targeted medicine controlled release system with a gold nanometer cage being a carrier. The problem that existing tumor treating medicine is large in side effect and poor in treating effect and the medication problem about photothermal therapy of tumors are effectively solved. The method includes the steps that, Re2O3, Fe(NO3)3, Mn(NO3)2, zinc(NO3)2, citric acid, ethylene glycol and the gold nanoparticle cage are added into HNO3, the pH is adjusted to 6, centrifuging is performed, sediment is redissolved through water, the pH is adjusted to 6, drying is performed, and the gold nanometer cage coated with manganese-zinc ferrite is obtained; thermosensitive materials are heated, medicine and the gold nanometer cage coated with the manganese-zinc ferrite are added in, centrifuging is performed, and gold nanometer cage-manganese-zinc ferrite compounds carrying the manganese-zinc ferrite and the thermosensitive materials are obtained; OPSS-PEG-SVA is added into a PBS buffering solution with -NH2 group target head molecules, the gold nanometer cage-manganese-zinc ferrite compounds are added in, centrifuging is performed, sediment is washed through the PBS buffering solution, and the combined targeted medicine controlled release system with the gold nanometer cage being the carrier is obtained. By means of the preparation method and the application, the purposes of thermal therapy, chemical (gene) treatment, targeting and controlled release can be achieved at the same time.

Description

technical field [0001] The invention relates to medicine, in particular to a preparation method and application of a compound targeting drug controlled release system with gold nanocage as a carrier. Background technique [0002] Gold nanocage is a hollow and porous gold nanomaterial with a very large light absorption cross section, which only requires 1.5mW / cm 2 The laser energy can effectively kill cancer cells without harming the surrounding cells that have not been irradiated. Therefore, the effect of gold nano cage particles in photothermal therapy is also stronger than that of gold nanoparticles with other structures; The cavity structure can be loaded with drugs, so that the drugs are in a relatively stable environment, reducing the influence of the complex environment in the body on the drugs; its surface is easy to modify, and can be combined with biomolecules such as organic polymers, antibodies, and ligands; in addition, it also has It has the characteristics of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K48/00A61K47/02A61K47/34A61K31/704A61K31/337A61K31/7105A61P35/00
Inventor 胡玉荣李春明李焕杰闫莉娟黄胜楠鲍士金邱晓静牛振喜张宁霞刘莹王卫萍
Owner ZHENGZHOU UNIV
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