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Preparation method and application of a compound targeted drug release system using gold nanocage as carrier

A technology of gold nanocage and drug controlled release, which is applied in the field of medicine and can solve the problems of poor treatment effect and large side effects

Inactive Publication Date: 2018-07-27
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In view of the above situation, in order to overcome the defects of the prior art, the purpose of the present invention is to provide a preparation method and application of a compound targeted drug controlled release system using gold nanocages as a carrier, which can effectively solve the problem of the large side effects of existing tumor treatment drugs. , Poor therapeutic effect, Medication problems of tumor photothermal therapy

Method used

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  • Preparation method and application of a compound targeted drug release system using gold nanocage as carrier

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Experimental program
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Effect test

Embodiment 1

[0022] In concrete implementation, the present invention can be realized by the following steps:

[0023] 1) Preparation of gold nanocages wrapped with manganese zinc ferrite:

[0024] At room temperature, with stirring at 1000rpm, the HNO 3 Add Re to 2 o 3 , Fe(NO 3 ) 3 , Mn(NO 3 ) 2 , Zn(NO 3 ) 2 , then add citric acid and ethylene glycol under magnetic stirring, the molar ratio is HNO 3 : Re 2 o 3 : Fe(NO 3 ) 3 :Mn(NO 3 ) 2 : Zn(NO 3 ) 2 : citric acid: ethylene glycol = 1: 0.07: 0.5: 0.5: 1.86: 1: 1, then according to HNO 3 Add gold nanocages with a molar ratio of 1000:1, adjust the pH to 6 with ammonium bicarbonate, sonicate the probe for 45min, add ultrapure water to 15mL, centrifuge at 15000rpm for 5min, discard the supernatant, add ultrapure water to the precipitate to 15mL, centrifuge at 15000rpm for 5min, discard the supernatant, and obtain the precipitate; then add ultrapure water to the precipitate to reach 15mL, repeat centrifugation for 3-5 times...

Embodiment 2

[0030] In concrete implementation, the present invention can also be realized by the following steps:

[0031]1) Preparation of gold nanocages wrapped with manganese zinc ferrite:

[0032] Under stirring at room temperature and a rotation speed of 1500rpm, the HNO 3 Add Re to 2 o 3 , Fe(NO 3 ) 3 , Mn(NO 3 ) 2 , Zn(NO 3 ) 2 , then add citric acid and ethylene glycol under magnetic stirring, the molar ratio is HNO 3 : Re 2 o 3 : Fe(NO 3 ) 3 :Mn(NO 3 ) 2 : Zn(NO 3 ) 2 : citric acid: ethylene glycol = 1: 0.07: 0.5: 0.5: 1.86: 1: 1, then according to HNO 3 Add gold nanocages with a molar ratio of 1000:1, adjust the pH to 6 with ammonium bicarbonate, sonicate the probe for 30 minutes, add ultrapure water to 10 mL, centrifuge at 15000 rpm for 5 minutes, discard the supernatant, add ultrapure water to the precipitate to 10mL, centrifuge at 15000rpm for 5 min, discard the supernatant, and obtain a precipitate; add ultrapure water to the precipitate to 10mL, repeat cen...

Embodiment 3

[0038] The present invention can also be realized by the following steps in concrete implementation:

[0039] 1) Preparation of gold nanocages wrapped with manganese zinc ferrite:

[0040] At room temperature, with stirring at 2000rpm, the HNO 3 Add Re to 2 o 3 , Fe(NO 3 ) 3 , Mn(NO 3 ) 2 , Zn(NO 3 ) 2 , then add citric acid and ethylene glycol under magnetic stirring, the molar ratio is HNO 3 : Re 2 o 3 : Fe(NO 3 ) 3 :Mn(NO 3 ) 2 : Zn(NO 3 ) 2 : citric acid: ethylene glycol = 1: 0.07: 0.5: 0.5: 1.86: 1: 1, then according to HNO 3 Add gold nanocages with a molar ratio of 1000:1, adjust the pH to 6 with ammonium bicarbonate, sonicate the probe for 30 minutes, add ultrapure water to 25 mL, centrifuge at 15000 rpm for 5 minutes, discard the supernatant, and add ultrapure water to the precipitate to 25mL, centrifuge at 15000rpm for 5 min, discard the supernatant, and obtain the precipitate; add ultrapure water to the precipitate to 25mL, repeat centrifugation for...

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Abstract

The invention relates to a preparation method and application of a composite targeting drug controlled release system using gold nano-cages as a carrier, and effectively solves the problems of existing tumor therapeutic drugs such as large side effects, poor therapeutic effect and tumor photothermal therapy. Add Re2O3, Fe(NO3)3, Mn(NO3)2, Zn(NO3)2, citric acid, ethylene glycol, gold nanocages to HNO3, adjust pH to 6, centrifuge, reconstitute the precipitate with water, adjust pH to 6 , dried to obtain gold nano-cages coated with manganese-zinc ferrite; heat the temperature-sensitive material, add drugs and gold nano-cages coated with manganese-zinc ferrite, and centrifuge to obtain manganese-zinc ferrite and temperature-sensitive materials gold nanocages-manganese-zinc ferrite complex; add OPSS-PEG-SVA into PBS buffer solution with primary amino target molecule, add gold nanocages-manganese-zinc ferrite complex, centrifuge, and precipitate with PBS buffer It can be washed with liquid to form a compound targeted drug controlled release system with gold nano cages as a carrier. The present invention simultaneously achieves the purposes of hyperthermia, chemical (gene) therapy, targeting and controlled release.

Description

technical field [0001] The invention relates to medicine, in particular to a preparation method and application of a compound targeting drug controlled release system with gold nanocage as a carrier. Background technique [0002] Gold nanocage is a hollow and porous gold nanomaterial with a very large light absorption cross section, requiring only 1.5mW / cm 2 The laser energy can effectively kill cancer cells without harming the surrounding cells that have not been irradiated. Therefore, the effect of gold nano cage particles in photothermal therapy is also stronger than that of gold nanoparticles with other structures; The cavity structure can be loaded with drugs, so that the drugs are in a relatively stable environment, reducing the influence of the complex environment in the body on the drugs; its surface is easy to modify, and can be combined with biomolecules such as organic polymers, antibodies, and ligands; in addition, it also has It has the characteristics of low ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K41/00A61K48/00A61K47/69A61K47/64A61K47/02A61K31/704A61K31/337A61K31/7105A61P35/00
Inventor 胡玉荣李春明李焕杰闫莉娟黄胜楠鲍士金邱晓静牛振喜张宁霞刘莹王卫萍
Owner ZHENGZHOU UNIV
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