Production method and application of electrode modified with gold nano-cage and myoglobin

Abstract

The invention discloses a production method and an application of an electrode modified with gold nano-cage and myoglobin. The production method comprises the following steps: taking graphite powder and an ionic liquid HPPF6 according to a mass ratio of (1.5-2.5):1, placing the graphite powder, the ionic liquid and liquid paraffin in a mortar, carrying out uniform grinding to obtain a carbon paste, filling a glass electrode tube with the carbon pate, and inserting a copper wire as a lead wire to obtain a carbon ionic liquid electrode CILE; dispensing 6-10 [mu]L of a AuNCs solution on the surface of the CILE, and naturally drying the CILE in a dark place under room temperature conditions to obtain an AuNCs / CILE electrode; dispensing 6-10 [mu]L of a 10-20 mg mL<-1> Mb solution on the surfaceof the AuNCs / CILE electrode, and naturally drying the AuNCs / CILE electrode in a dark place at room temperature to obtain a Mb / AuNCs / CILE electrode; and dispensing 4-8 [mu]L of a 0.3-0.7% Nafion ethanol solution on the surface of the Mb / AuNCs / CILE electrode, and drying the Mb / AuNCs / CILE electrode in a dark place at room temperature to obtain the Nafion / Mb / AuNCs / CILE electrode. The modified electrode has a good electrocatalytic reduction effect on trichloroacetic acid, sodium nitrite and hydrogen peroxide, has the advantages of wide linear range, low detection limit and high sensitivity, and can be well applied to the detection of the content of above three substances in an object.

Description

technical field [0001] The invention relates to the technical field of biochemically modified electrodes, in particular to a preparation method and application of a gold nanocage and a myoglobin modified electrode. Background technique [0002] The detection of biochemical reagents plays a key role in applications such as biomedical forensic identification and environmental science. The development of highly sensitive, economical, and miniature sensors requires advanced technology and basic knowledge of chemistry, biology, and material science. In general, the sensor features two functional parts; a recognition element for selective or specific binding of the target analyte and a transducer part for signaling the binding reaction. When it comes to response time, signal-to-noise (S / N) selectivity, limit of detection (LOD) of the recognition process, an efficient sensor relies heavily on these two parts. Designing efficient sensors therefore relies on novel materials to impro...

AI Technical Summary

Problems solved by technology

[0005] Hydrogen peroxide is widely used in electronics, light industry, food, environmental protection, medicine and other fields, such as sterilization, textile bleaching, industrial wastewater treatment, cosmetics, food and dairy production equipment and production pipeline cleaning, etc. But at the same time, hydrogen peroxide may also cause various diseases and bring hidden dangers to people's life and health.

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