Production method and application of electrode modified with gold nano-cage and myoglobin

A myoglobin and modified electrode technology, which is applied in the field of biochemically modified electrodes, can solve problems such as hidden dangers to life and health, and achieve the effects of low detection limit, excellent conductivity and biocompatibility, and high sensitivity

Inactive Publication Date: 2018-04-20
HAINAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Hydrogen peroxide is widely used in electronics, light industry, food, environmental protection, medicine and other fields, such as sterilization, textile bleaching, industrial wastewater treatment, ...

Method used

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  • Production method and application of electrode modified with gold nano-cage and myoglobin
  • Production method and application of electrode modified with gold nano-cage and myoglobin
  • Production method and application of electrode modified with gold nano-cage and myoglobin

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Embodiment 1

[0045] A preparation method of a gold nanocage and a myoglobin modified electrode, comprising the following steps:

[0046] S1. Take graphite powder and ionic liquid HPPF according to the mass ratio of 1.5:1 6 , namely take 1.5g graphite powder, 1.0g ionic liquid HPPF 6 Grind with 500 μL of liquid paraffin in a mortar for 2.5 hours to obtain a carbon paste, then fill the carbon paste into a 4mm glass electrode tube for compaction, and insert a copper wire as a wire to obtain a carbon ionic liquid electrode CILE;

[0047] Before each electrode modification, the electrode surface needs to be polished to a mirror surface on a polishing paper;

[0048] S2. Take 6 μL of AuNCs solution and drop-coat it on the surface of CILE, and dry it naturally in the dark at room temperature to obtain an AuNCs / CILE electrode;

[0049] S3. Take another 10 μL of 10 mg mL -1 The Mb solution was drip-coated on the surface of the AuNCs / CILE electrode, and dried naturally at room temperature in the ...

Embodiment 2

[0052] A preparation method of a gold nanocage and a myoglobin modified electrode, comprising the following steps:

[0053] S1. Take graphite powder and ionic liquid HPPF according to the mass ratio of 2.5:1 6 , namely take 2.5g graphite powder, 1.0g ionic liquid HPPF 6 Grind with 700 μL of liquid paraffin in a mortar for 4 hours to obtain a carbon paste, then fill the carbon paste into a 4 mm glass electrode tube for compaction, and insert a copper wire as a wire to obtain a carbon ionic liquid electrode CILE;

[0054] Before each electrode modification, the electrode surface needs to be polished to a mirror surface on a polishing paper;

[0055] S2. Take 10 μL of the AuNCs solution and drop-coat it on the surface of the CILE, and dry it naturally in the dark at room temperature to obtain the AuNCs / CILE electrode;

[0056] S3, take another 6 μL 20mg mL -1 The Mb solution was drip-coated on the surface of the AuNCs / CILE electrode, and dried naturally at room temperature in ...

Embodiment 3

[0059] A preparation method of a gold nanocage and a myoglobin modified electrode, comprising the following steps:

[0060] S1. Take graphite powder and ionic liquid HPPF according to the mass ratio of 2:1 6 , namely take 1.6g graphite powder, 0.8g ionic liquid HPPF 6 Grind with 500 μL of liquid paraffin in a mortar for 3 hours to obtain a carbon paste, then fill the carbon paste into a 4 mm glass electrode tube for compaction, and insert a copper wire as a wire to obtain a carbon ionic liquid electrode CILE;

[0061] Before each electrode modification, the electrode surface needs to be polished to a mirror surface on a polishing paper;

[0062] S2. Take 8 μL of AuNCs solution and drop-coat it on the surface of CILE, and dry it naturally in the dark at room temperature to obtain an AuNCs / CILE electrode;

[0063] S3. Take another 8 μL of 15 mg mL -1 The Mb solution was drip-coated on the surface of the AuNCs / CILE electrode, and dried naturally at room temperature in the dark...

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Abstract

The invention discloses a production method and an application of an electrode modified with gold nano-cage and myoglobin. The production method comprises the following steps: taking graphite powder and an ionic liquid HPPF6 according to a mass ratio of (1.5-2.5):1, placing the graphite powder, the ionic liquid and liquid paraffin in a mortar, carrying out uniform grinding to obtain a carbon paste, filling a glass electrode tube with the carbon pate, and inserting a copper wire as a lead wire to obtain a carbon ionic liquid electrode CILE; dispensing 6-10 [mu]L of a AuNCs solution on the surface of the CILE, and naturally drying the CILE in a dark place under room temperature conditions to obtain an AuNCs/CILE electrode; dispensing 6-10 [mu]L of a 10-20 mg mL<-1> Mb solution on the surfaceof the AuNCs/CILE electrode, and naturally drying the AuNCs/CILE electrode in a dark place at room temperature to obtain a Mb/AuNCs/CILE electrode; and dispensing 4-8 [mu]L of a 0.3-0.7% Nafion ethanol solution on the surface of the Mb/AuNCs/CILE electrode, and drying the Mb/AuNCs/CILE electrode in a dark place at room temperature to obtain the Nafion/Mb/AuNCs/CILE electrode. The modified electrode has a good electrocatalytic reduction effect on trichloroacetic acid, sodium nitrite and hydrogen peroxide, has the advantages of wide linear range, low detection limit and high sensitivity, and can be well applied to the detection of the content of above three substances in an object.

Description

technical field [0001] The invention relates to the technical field of biochemically modified electrodes, in particular to a preparation method and application of a gold nanocage and a myoglobin modified electrode. Background technique [0002] The detection of biochemical reagents plays a key role in applications such as biomedical forensic identification and environmental science. The development of highly sensitive, economical, and miniature sensors requires advanced technology and basic knowledge of chemistry, biology, and material science. In general, the sensor features two functional parts; a recognition element for selective or specific binding of the target analyte and a transducer part for signaling the binding reaction. When it comes to response time, signal-to-noise (S / N) selectivity, limit of detection (LOD) of the recognition process, an efficient sensor relies heavily on these two parts. Designing efficient sensors therefore relies on novel materials to impro...

Claims

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Application Information

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IPC IPC(8): G01N27/327
CPCG01N27/308G01N27/3277
Inventor 孙伟陈玮翁文举殷春晓李晓燕赵文舒
Owner HAINAN NORMAL UNIV
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