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Preparation and application of ZnO@CdTe-carboxylation C3N4 photoelectric DNA sensor

A C3N4, carboxylation technology, used in instruments, scientific instruments, material analysis by electromagnetic means, etc., to achieve the effect of improving optoelectronic properties, promoting optoelectronic properties, and enhancing optoelectronic properties

Inactive Publication Date: 2015-01-21
UNIV OF JINAN
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This status quo is far from meeting the needs of society, and there will be a big leap forward

Method used

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Embodiment 1

[0036] Example 1 A ZnO CdTe-carboxylated C 3 N 4 Preparation method of photoelectric DNA sensor

[0037](1) A 1.0 cm×2.5 cm rectangular ITO conductive glass was ultrasonically cleaned with acetone, ultrapure water, and ethanol for 30 min, then dried with pure nitrogen, and used as a working electrode, platinum wire as a counter electrode, and a saturated calomel electrode As a reference electrode, it constitutes a three-electrode electrolytic cell;

[0038] (2) A layer of ZnO nanorods is grown on the surface of the ITO electrode;

[0039] (3) The working electrode prepared in (2) was immersed in the CdTe quantum dot solution, and a layer of CdTe was deposited on the ZnO nanorods on the surface of the working electrode by using the current-time method with a potential of -1.0 V and a deposition time of 200 s. Quantum dots, dry at room temperature;

[0040] (4) Drop 5 μL of carboxylated C 3 N 4 (3) to the surface of the modified working electrode, and dry in a refrigerat...

Embodiment 2

[0043] Example 2 A ZnO CdTe-carboxylated C 3 N 4 Preparation method of photoelectric DNA sensor

[0044] (1) A 1.0 cm×2.5 cm rectangular ITO conductive glass was ultrasonically cleaned with acetone, ultrapure water, and ethanol for 30 min, then dried with pure nitrogen, and used as a working electrode, platinum wire as a counter electrode, and a saturated calomel electrode As a reference electrode, it constitutes a three-electrode electrolytic cell;

[0045] (2) A layer of ZnO nanorods is grown on the surface of the ITO electrode;

[0046] (3) The working electrode prepared in (2) was immersed in the CdTe quantum dot solution, and a layer of CdTe was deposited on the ZnO nanorods on the surface of the working electrode by using the current-time method with a potential of -1.0 V and a deposition time of 300 s. Quantum dots, dry at room temperature;

[0047] (4) Drop-coat 15 μL of carboxylated C 3 N 4 (3) to the surface of the modified working electrode, and dry in a ref...

Embodiment 3

[0050] Example 3 A ZnO CdTe-carboxylated C 3 N 4 Preparation method of photoelectric DNA sensor

[0051] (1) A 1.0 cm×2.5 cm rectangular ITO conductive glass was ultrasonically cleaned with acetone, ultrapure water, and ethanol for 30 min, then dried with pure nitrogen, and used as a working electrode, platinum wire as a counter electrode, and a saturated calomel electrode As a reference electrode, it constitutes a three-electrode electrolytic cell;

[0052] (2) A layer of ZnO nanorods is grown on the surface of the ITO electrode;

[0053] (3) The working electrode prepared in (2) was immersed in the CdTe quantum dot solution, and a layer of CdTe was deposited on the ZnO nanorods on the surface of the working electrode by using the current-time method with a potential of -1.0 V and a deposition time of 400 s. Quantum dots, dry at room temperature;

[0054] (4) Drop 20 μL of carboxylated C 3 N 4 (3) to the surface of the modified working electrode, and dry in a refriger...

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PUM

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Abstract

The invention relates to preparation and application of a ZnO@CdTe-carboxylation C3N4 photoelectric DNA sensor, and belongs to the technical field of biosensing detection. A ZnO@CdTe-carboxylation C3N4 compound is taken as a beacon material; qualitative and quantitative detection on the target DNA and the housekeeping gene of a physical tissue can be realized; the advantages of simple equipment, low cost and easiness in miniaturization are achieved.

Description

technical field [0001] The invention relates to the preparation and application of a ZnO CdTe-carboxylated C3N4 photoelectric DNA sensor. The sensor is used for the detection of biological tissue DNA and belongs to the technical field of biosensing detection. Background technique [0002] The United States is the first country in the world to use genetic testing to predict diseases. In 2005, nearly 5 million people were tested. Through predictive and medical intervention, the incidence of colorectal cancer has dropped by 90%, and the incidence of breast cancer has dropped by 70%. %. China is relatively lagging behind in genetic testing. By the end of 2006, only about 100,000 people had been tested. In China, the number of cancer patients in the past five years has reached 7.5 million, and the incidence of cancer has increased by more than 29% in less than 20 years. About 1.6 million people die of cancer every year. According to statistics, the economic loss caused by disea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/416G01N27/327G01N33/58
Inventor 庞雪辉吴丹闫涛范大伟胡丽华魏琴任伟王友英
Owner UNIV OF JINAN
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