Preparation method of electrochemical bladder cancer DNA sensor

An electrochemical and sensor technology, applied in the field of bladder cancer DNA detection

Inactive Publication Date: 2015-01-21
TIANJIN POLYTECHNIC UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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  • Preparation method of electrochemical bladder cancer DNA sensor
  • Preparation method of electrochemical bladder cancer DNA sensor
  • Preparation method of electrochemical bladder cancer DNA sensor

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Embodiment 1

[0024] 1. Preparation of electrochemical bladder cancer DNA sensor:

[0025] 1.1 Carboxylation of glassy carbon electrodes: Glassy carbon electrodes should be pretreated before use. The glassy carbon electrode was polished in alumina slurry with a particle size of 50nm for 5 minutes to form a mirror surface, and then ultrasonically cleaned in 0.1mol / L nitric acid, absolute ethanol and ultrapure water for 3 minutes to remove impurities on the surface of the electrode. Blow dry the surface of the electrode with pure nitrogen. The cleaned glassy carbon electrode was activated by cyclic voltammetry in a 0.5mol / L sulfuric acid solution through an electrochemical workstation. After meeting the requirements, it was soaked in ultrapure water for later use. The above-mentioned treated glassy carbon electrode was immersed in 0.1mol / L sodium hydroxide solution for surface carboxylation, and cyclic voltammetry was used to scan for 1 circle, with a scanning speed of 50mV / s and a scanning ...

Embodiment 2

[0032] 1. Preparation of electrochemical bladder cancer DNA sensor:

[0033] 1.1 Carboxylation of glassy carbon electrodes: Glassy carbon electrodes should be pretreated before use. Polish the glassy carbon electrode in alumina slurry with a particle size of 50nm for 10 minutes to form a mirror surface, then ultrasonically clean it in 1mol / L nitric acid, absolute ethanol and ultrapure water for 5 minutes to remove impurities on the electrode surface, and finally use high-purity Dry the surface of the electrode with nitrogen gas. The cleaned glassy carbon electrode was activated by cyclic voltammetry in a 0.1mol / L sulfuric acid solution through an electrochemical workstation. After meeting the requirements, it was soaked in ultrapure water for later use. The above-mentioned treated glassy carbon electrode was immersed in 1mol / L sodium hydroxide solution for surface carboxylation, and was scanned by cyclic voltammetry for 5 cycles at a scanning speed of 100mV / s and a scanning r...

Embodiment 3

[0040] 1. The preparation of the electrochemical bladder cancer DNA sensor comprises the following two steps:

[0041] 1.1 Carboxylation of glassy carbon electrodes: Glassy carbon electrodes should be pretreated before use. Polish the glassy carbon electrode in alumina slurry with a particle size of 50nm for 20 minutes to form a mirror surface, then ultrasonically clean it in 1mol / L nitric acid, absolute ethanol and ultrapure water for 10 minutes to remove impurities on the electrode surface, and finally use high-purity Dry the surface of the electrode with nitrogen gas. The cleaned glassy carbon electrode was activated by cyclic voltammetry in a 1.0mol / L sulfuric acid solution through an electrochemical workstation. After meeting the requirements, it was soaked in ultrapure water for later use. The above-mentioned treated glassy carbon electrode was immersed in 0.8mol / L sodium hydroxide solution for surface carboxylation, and cyclic voltammetry was used to scan 10 cycles at ...

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Abstract

According to the technical scheme of an electrochemical bladder cancer DNA sensor, carboxylation is carried out on the surface of a glassy carbon electrode by an electrochemical method, and a bladder cancer cell specific DNA probe is assembled on the glassy carbon electrode to prepare the bladder cancer DNA sensor. The glassy carbon electrode is a base electrode, and electrode carboxylation and probe single-stranded DNA assembling are successively carried out. By electrode carboxylation, the probe DNA is fixed. The probe DNA is an recognition element, and a biological bridging agent is 1-ethyl-3-(3-dimethyl aminopropyl)carbodiimide and N-hydroxy succinimide. The preparation method is characterized in that the electrochemical bladder cancer DNA sensor with high sensitivity is obtained. The sensor has high sensitivity, high stability and good selectivity, and testing results of the sensor are better than those of a traditional DNA detection method. The preparation method comprises: 1, preparation of the electrochemical bladder cancer DNA sensor; 2, hybridization of the electrochemical bladder cancer DNA sensor and a target DNA; and 3, electrochemical signal detection of the sensor. The sensor has a simple operation method and is convenient for practical popularization and application.

Description

technical field [0001] The invention relates to bladder cancer DNA detection technology, in particular to a preparation method of an electrochemical bladder cancer DNA sensor. The sensor organically combines nucleic acid hybridization technology with electrochemical technology, and detects the specific DNA of bladder cancer through the peak signal of cyclic voltammetry (CV) and differential pulse (DPV) method. Background technique [0002] Deoxyribonucleic acid (DNA) is one of the most important basic biomolecules for all living organisms. Therefore, the identification and quantitative analysis of DNA sequences are of great significance in the fields of gene diagnosis, disease prevention and microbial detection. In recent years, with the rapid development of biotechnology and great progress in electronic information technology, DNA sequence detection technology has also advanced by leaps and bounds, and the research on detection methods has also made significant progress. ...

Claims

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Application Information

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IPC IPC(8): G01N27/327
Inventor 许世超张晨张雪平黄丹丹王才富郑永彬
Owner TIANJIN POLYTECHNIC UNIV
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