A
nanopore sequencing circuit unit (200) and a
gene sequencing device. The
nanopore sequencing circuit unit (200) is implemented by using a
CMOS circuit, and is used for detecting a bidirectional micro-current
signal of a
nanopore (201). The circuit unit comprises: a nanopore clamping circuit, which is used for stabilizing the
voltage of a detection
electrode positioned on one side of the nanopore (201), so as to generate a fixed
voltage difference with a common
electrode (202) positioned on the other side of the nanopore (201), and to form a charging and discharging path with an integral reset circuit, driving individual
nucleotide molecules to pass through the nanopore (201) one by one by means of the
voltage difference; the integral reset circuit, which is used for, when the nanopore clamping circuit constitutes the charging and discharging path, performing integral amplification on a bidirectional micro-current
signal of the nanopore (201) , and converting the bidirectional micro-current
signal into a voltage signal; and an output circuit, which is used for receiving the voltage signal converted by the integral reset circuit, and outputting the voltage signal. A high-
throughput gene sequencing device having bidirectional detection capability is constructed on the basis of a small-area sequencing circuit unit, such that the detection precision and efficiency are improved.