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Ionic liquid functional graphene vibrio DNA electrochemical sensor and preparation method and application thereof

A technology of ionic liquid and vibrio graphene, which is applied in the field of bioelectrochemical sensors to achieve high sensitivity, increase specific surface area, and enhance immobilization ability

Active Publication Date: 2018-12-25
QINGDAO UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the construction of Vibrio DNA sensor based on IL-GR-ZIF-8 complex membrane-modified gold electrode immobilized probe ssDNA and used to detect Vibrio parahaemolyticus

Method used

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  • Ionic liquid functional graphene vibrio DNA electrochemical sensor and preparation method and application thereof
  • Ionic liquid functional graphene vibrio DNA electrochemical sensor and preparation method and application thereof
  • Ionic liquid functional graphene vibrio DNA electrochemical sensor and preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0030] (1) Preparation of IL-GR-ZIF-8 complex

[0031] First, GO was prepared by the Hummer method, and then a certain amount of GO was dispersed in deionized water, and ultrasonically dispersed for 2 hours to obtain a GO dispersion with a concentration of 0.5-1.0 mg / mL; Zn(NO 3 ) 2 ·6H 2 O methanol solution was slowly and uniformly added dropwise to the above GO dispersion; under the same conditions, 10 mL of 2-methylimidazole methanol solution with a concentration of 900 mg / mL was added dropwise to the above mixture, stirred for 15 min, and aged for 24 h at the same temperature. The off-white precipitate was collected by centrifugation, washed three times with water and methanol, and dried naturally to obtain the GO-ZIF-8 complex; weigh 40 mg of the GO-ZIF-8 complex and disperse it in deionized water to make the concentration 1.0 mg / mL, slowly add 20mg IL and 20mg KOH, then sonicate for 30min; heat and stir the sonicated dispersion at 80°C for 24h, centrifuge at 4000rpm ...

Embodiment 2

[0037] (1) Preparation of IL-GR-ZIF-8 complex

[0038] Prepared according to the method and conditions of step (a) in Example 1;

[0039] (2) Preparation of IL-GR-ZIF-8 modified gold electrode

[0040] Gold electrode via 0.05μm A1 2 o 3 After the polishing powder is polished, it is rinsed with double distilled water, ultrasonicated in an ultrasonic water bath for 5 min, and dried with high-purity nitrogen; the IL-GR-ZIF-8 complex obtained in step (1) is ultrasonically dispersed in a deionized medium , prepared into a dispersion with a concentration of 3 mg / mL, and 8 μL of the dispersion was drip-coated on the surface of the treated gold electrode, and dried naturally at room temperature to obtain IL-GR-ZIF-8 / Au;

[0041] (3) Preparation of DNA sensor

[0042] Immerse IL-GR-ZIF-8 / Au in a mixed solution of 0.4mol / L EDC and 0.1mol / L NHS for 30min to carboxylate the surface of the modified electrode; take 40μL concentration as 1×10 -6 The mol / L probe ssDNA solution was drip-c...

Embodiment 3

[0044] (1) Preparation of IL-GR-ZIF-8 complex

[0045] Prepared according to the method and conditions of step (a) in Example 1;

[0046] (2) Preparation of IL-GR-ZIF-8 modified gold electrode

[0047] Gold electrode via 0.05μm A1 2 o 3 After the polishing powder is polished, it is rinsed with double distilled water, ultrasonicated in an ultrasonic water bath for 5 min, and dried with high-purity nitrogen; the IL-GR-ZIF-8 complex obtained in step (1) is ultrasonically dispersed in a deionized medium , prepared into a dispersion with a concentration of 3 mg / mL, and 10 μL of the dispersion was drip-coated on the surface of the treated gold electrode, and dried naturally at room temperature to obtain IL-GR-ZIF-8 / Au;

[0048] (3) Preparation of DNA sensor

[0049] Immerse IL-GR-ZIF-8 / Au in a mixed solution of 0.4mol / L EDC and 0.1mol / L NHS for 30min to carboxylate the surface of the modified electrode; -6 The mol / L probe ssDNA solution was drip-coated on the surface of the mod...

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Abstract

The invention discloses an ionic liquid functional graphene vibrio DNA electrochemical sensor and a preparation method and application thereof. Firstly, ZIF-8 is prepared in graphene oxide dispersion;then a graphene-ZIF-8 compound is subjected to covalent modification by amino functional ionic liquid to prepare an ionic liquid functional graphene-ZIF-8 compound; a gold electrode modified by the compound is prepared by a dispensing method; and then vibrio probe ssDNA is fixed on the surface of the modified electrode by a biological linker so as to prepare the vibrio DNA sensor. The DNA sensorhas excellent sensitivity and selectivity, a low detection limit and a wide linear range. The preparation method comprises: preparation of an electrode modification material; preparation of an electrochemical vibrio parahaemolyticus DNA sensor; hybridization of the electrochemical vibrio parahaemolyticus DNA sensor and target DNA; and sensor electrochemical signal detection. The sensor is simple in operation method and has potential application value.

Description

Technical field: [0001] The invention belongs to the technical field of bioelectrochemical sensors, and in particular relates to an ionic liquid functionalized graphene vibrio DNA electrochemical sensor and a preparation method thereof, and specific DNA for highly sensitive detection of pathogenic vibrio parahaemolyticus. Background technique: [0002] Marine Vibrio is a kind of rod-shaped or arc-shaped Gram-negative bacteria with strong activity, which is widely distributed in brackish water, coastal areas, estuaries, marine water bodies, sediments and marine organisms, and is distributed in the global estuary environment. Gram-negative bacteria that can cause acute gastroenteritis in humans when raw, undercooked, or mishandled seafood is consumed. Among them, Vibrio parahaemolyticus is the most harmful. The study found that the tlh gene was found in all strains of Vibrio parahaemolyticus, so it was considered as a useful indicator for detecting total Vibrio parahaemolyticu...

Claims

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Application Information

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IPC IPC(8): G01N27/327
CPCG01N27/3277G01N27/3278
Inventor 詹天荣王闰夏田夏
Owner QINGDAO UNIV OF SCI & TECH
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