Composite film modified DNA sensor and its preparation method and application in detection of lignin peroxidase (Lip) specific coding gene segment

A composite membrane and sensor technology, applied in the field of biosensors, can solve problems such as reducing the detection accuracy of gene sensing technology, and achieve the effects of enhancing electronic conductivity, low cost, and low detection.

Inactive Publication Date: 2011-11-23
HUNAN UNIV
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  • Abstract
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Problems solved by technology

But there is a problem that cannot be ignored: the electron conductivity of carbon paste electrodes is significantly lower than that of gold electrodes, which will reduce the detection accuracy of gene sensing technology

Method used

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  • Composite film modified DNA sensor and its preparation method and application in detection of lignin peroxidase (Lip) specific coding gene segment
  • Composite film modified DNA sensor and its preparation method and application in detection of lignin peroxidase (Lip) specific coding gene segment
  • Composite film modified DNA sensor and its preparation method and application in detection of lignin peroxidase (Lip) specific coding gene segment

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Embodiment Construction

[0032] The present invention will be further described below in combination with specific embodiments and accompanying drawings.

[0033] a kind of like figure 1 The shown composite membrane modified DNA sensor includes a carbon paste electrode, the carbon paste electrode includes a carbon rod 3 and a PTFE tube 2, the carbon rod 3 is embedded in the PTFE tube 2, and one end of the carbon rod 3 leads out of the PTFE tube 2 through an electric wire 1, The other end of the carbon rod 3 is in contact with a magnet 4, and the magnet 4 is arranged at one end of the carbon rod 3 and 8mm away from the mouth of the PTFE tube 2, and the carbon paste 5 is filled between the magnet 4 and the mouth of the PTFE tube 2, and the carbon paste electrode The sensing end of the sensor is coated with a sensitive substance 6, and the sensitive substance 6 includes a composite membrane and a sulfhydrylated DNA capture probe (the sequence is 5'-HS(CH 2 ) 6 TTGTTGACGAAGGACTGCCA-3′), the composite f...

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Abstract

The invention discloses a composite film modified DNA sensor. The DNA sensor is characterized in that the sensor comprises a carbon paste electrode; the carbon paste electrode comprises a carbon rod, a Teflon tube, a magnet, and carbon paste; an induction end of the carbon paste electrode is coated with a sensitive substance; the sensitive substance comprises a composite film and a DNA capture probe; magnetic nanoparticles, multi-walled carbon nanoscale tube-gold nanoparticles and chitosan are utilized for modifying orderly the surface of the carbon paste electrode and compose the composite film; and the DNA capture probe is utilized for modifying the surface of the multi-walled carbon nanoscale tube-gold nanoparticles. The invention also discloses a preparation method of the DNA sensor. The preparation method comprises the processing steps of manufacturing a carbon paste electrode, modifying the surface of an induction end of the manufactured carbon paste electrode by a sensitive substance, and the like. The invention further discloses an application of the DNA sensor in detecting a lignin peroxidase (Lip) specific coding gene segment. Because a sensitive substance is utilized for modifying the surface of a carbon paste electrode of the DNA sensor, the DNA sensor has a strong capacity of electron conduction and a high accuracy of detection. The preparation method has the advantages of low cost and simple process.

Description

technical field [0001] The invention relates to the field of biosensors, in particular to a membrane-modified biosensor and its preparation method and application. Background technique [0002] The use of biosensing technology to detect gene fragments is a trend in gene analysis and detection, among which electrochemical sensors have received extensive attention because of their advantages of fast response, high sensitivity, high selectivity and strong operability. For DNA sensors, common working electrodes are gold electrodes or screen-printed electrodes based on gold nanoparticles, because gene probes modified with sulfhydryl groups can be easily assembled on the electrode surface through the cross-linking of sulfhydryl groups with gold for related detection. However, gold electrodes are expensive, and screen-printed electrodes are disposable, and the total cost for high-volume assays can be significant. In recent years, with the rapid combination of new nanomaterials and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/327C12Q1/68C12Q1/28
Inventor 章毅曾光明晏铭汤琳黎媛萍刘灿李贞
Owner HUNAN UNIV
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