Electrogenerated chemiluminescence sensor with quantum dot modified nano porous carbon paste electrode for testing trace antibiotic residue

A nanoporous gold and carbon paste electrode technology is used in electrochemiluminescence sensors to detect antibiotic residues in milk and meat products. , the effect of increasing the utilization efficiency and improving the sensitivity

Inactive Publication Date: 2011-05-25
UNIV OF JINAN
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Problems solved by technology

[0005] 1. Immunoassay has the advantages of high sensitivity and specificity, but the immunological reagents are expensive and the detection cost is high, which limits the application of immunoassay in the detection of antibiotic residues
[0006] 2. UV-Vis spectrophotometry and fluorescence method are simple, low cost and wide linear range, but their sensitivity and selectivity are not high, so the concentration used is limited to 10 -6 level, so the use of UV detectors to determine antibiotic residue samples generally needs to be concentrated to meet the requirements
[0007] 3. High-performance liquid chromatography is a commonly used method for detecting antibiotic residues. These two methods have high sensitivity and can also solve certain selectivity problems. However, these two methods are cumbersome to operate, and the analysis process takes a long time. Now high-throughput testing needs
[0008] 4. The chemiluminescence method is a new method developed in recent years. It is also used in the detection of antibiotic residues. It has high sensitivity and a small amount of samples. It can be used in combination with flow injection technology to realize instrument automation. However, the choice of method Poor performance, can not solve the interference problem of coexisting substances, so the test results will bring the results of the interference of coexisting substances
[0009] 5. For the detection and analysis of antibiotic residues, the above methods generally have disadvantages such as high detection cost, false positive results, complicated detection process, single detection, large amount of reagents, not suitable for rapid on-site detection, and large interference of coexisting substances. Can not meet the needs of actual testing

Method used

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  • Electrogenerated chemiluminescence sensor with quantum dot modified nano porous carbon paste electrode for testing trace antibiotic residue
  • Electrogenerated chemiluminescence sensor with quantum dot modified nano porous carbon paste electrode for testing trace antibiotic residue
  • Electrogenerated chemiluminescence sensor with quantum dot modified nano porous carbon paste electrode for testing trace antibiotic residue

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Embodiment 1

[0036] Example 1 (tetracyclines, such as tetracycline)

[0037] A method for preparing a quantum dot modified nanoporous gold-carbon paste electrode electrochemiluminescence sensor for detecting trace tetracycline residues, comprising the following steps:

[0038] (1) Synthesizing a nanoporous gold material and a luminescent quantum dot solution according to existing methods;

[0039] (2) Hydroxylation of the surface of nanoporous gold: first, ultrasonically clean with deionized water, acetone and ethanol, then blow dry with high-purity nitrogen, immerse in 4% NaOH solution for 20min, then clean with deionized water, and dry at 120°C 1.5 hours, so that uniform hydroxyl groups are generated on the surface and pores of nanoporous gold;

[0040] (3) Immerse the nanoporous gold modified with hydroxyl groups in a toluene solution of triaminopropyltrimethoxysilane (APS) for 5 minutes, then rinse with toluene, and blow dry with nitrogen;

[0041] (4) Immerse the nanoporous gold obt...

Embodiment 2

[0051] Embodiment 2 (macrolides, such as erythromycin)

[0052] A method for preparing a quantum dot modified nanoporous gold-carbon paste electrode electrochemiluminescence sensor for detecting trace erythromycin residues, comprising the following steps:

[0053] (1) Synthesizing a nanoporous gold material and a luminescent quantum dot solution according to existing methods;

[0054] (2) Hydroxylation of nanoporous gold surface: first, ultrasonically clean with deionized water, acetone and ethanol, then blow dry with high-purity nitrogen, immerse in 4% NaOH solution for 30min, then clean with deionized water, and dry at 120°C 1 hour, so that uniform hydroxyl groups are generated on the surface and pores of the nanoporous gold;

[0055] (3) Immerse the nanoporous gold modified with hydroxyl groups into a toluene solution of triaminopropyltrimethoxysilane (APS) for 10 minutes, then rinse with toluene, and blow dry with nitrogen;

[0056] (4) Immerse the nanoporous gold obtain...

Embodiment 3

[0066] Embodiment 3 (chloramphenicols, such as chloramphenicol)

[0067] A method for preparing a quantum dot modified nanoporous gold-carbon paste electrode electrochemiluminescence sensor for detecting trace amounts of chloramphenicol residues, comprising the following steps:

[0068] (1) Synthesizing a nanoporous gold material and a luminescent quantum dot solution according to existing methods;

[0069] (2) Hydroxylation of nanoporous gold surface: first, ultrasonically clean with deionized water, acetone and ethanol, then blow dry with high-purity nitrogen, immerse in 4% NaOH solution for 25min, then clean with deionized water, and dry at 120°C 2 hours, so that uniform hydroxyl groups are generated in the nanoporous gold surface and pores;

[0070] (3) Immerse the nanoporous gold modified with hydroxyl groups in a toluene solution of diaminopropyltrimethoxysilane (APS) for 8 minutes, then rinse with toluene, and blow dry with nitrogen;

[0071] (4) Immerse the nanoporou...

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Abstract

The present invention discloses a preparation method of an electrogenerated chemiluminescence sensor with a quantum dot modified nano porous carbon paste electrode for detecting trace antibiotic residues. The preparation method for the carbon paste electrode comprises the following steps: synthesizing nanoporous gold and quantum dot solution according to the existing method; modifying light-emitting quantum dots into the surface and the porous channels of the nanoporous gold material layer by layer by adopting the layer-by-layer self-assembly modification technology; doping the modified nanoporous gold material into graphite powder. The method for detecting the trace antibiotic residues comprises the following steps: connecting the modified carbon paste electrode with an electrogenerated chemiluminescence instrument, and detecting the trace antibiotic residues in dairy and meat products. The sensor has high sensitivity and a high detection speed, and one basic detection process can be completed within only 2-5 minutes. Moreover, the sensor has a low cost, and is economical and practical. The instrument is easy to operate and can automatically record and analyze experimental results, the effect of subjective factors is avoided, and good repeatability is achieved.

Description

technical field [0001] The present invention relates to the technical field of detection of antibiotic residues, and more specifically relates to the preparation of a highly sensitive electrochemiluminescence sensor for detecting trace antibiotic residues. The present invention also relates to the detection of milk, meat, Methods for antibiotic residues in articles. Background technique [0002] Antibiotics are also called antibiotics. Antibiotics are substances produced by bacteria, fungi or other microorganisms in the course of life, and can also be made into similar substances by chemical semi-synthesis (called synthetic antibiotics). Mainly include tetracyclines, macrolides, chloramphenicol, β-lactams, aminoglycosides, lincomycins, antifungal antibiotics and antitumor antibiotics. Antibiotics have the effect of inhibiting or killing pathogenic microorganisms such as bacteria, spirochetes, and chlamydia at low concentrations. Since the 1950s, when antibiotics were used ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/66
Inventor 于京华万夫伟王少伟颜梅程晓亮续晶华孙旦子李志英
Owner UNIV OF JINAN
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