34results about How to "Increase screening throughput" patented technology

The invention relates to the field of genetic resource conservation, particularly relates to the field of animal paternity identification, and discloses a method of establishing an animal paternity identification system on basis of whole genome STR. The method comprises the following steps: 1) looking up whole genome STR loci; 2) preliminarily screening the STR loci; 3) carrying out a primer design; 4) randomly sampling animal populations and extracting genome DNA; 5) verifying the population diversity of the STR loci; 6) carrying out Hardy-Weinberg equilibrium inspection on the STR loci with genetic polymorphism, and screening out the STR loci with the genotype frequency distribution which meets the Hardy-Weinberg equilibrium; 7) evaluating the forensic medicine application value of the STR loci; 8) establishing the STP animal paternity identification system. The method is convenient to operate; the constructed paternity identification system has a paternity exclusion probability of more than 0.99, and is capable of accurately carrying out animal paternity identification, therefore, the paternity identification system can be used for guiding the breeding of rare species, and the identification and breeding of economic species.

The invention relates to a phage antibody library which assembles an ScFv gene fragment between Restriction Enzyme cutting sites of SfiI and NotI of a pCANTAB5E carrier. The phage antibody library is characterized in that the ScFv gene fragment can be affinitive and enriched with antigens of 16-membered macrolide agricultural chemicals to form a soluble single-chain antibody and the phage antibody library is applied to immunoassay of pesticide residue. The phage antibody library has the advantages that the antibody with high affinity can be obtained without animal immunization, the test period is short, the antibody library is the phage antibody library which takes small molecular milbemycin oxime of a 16-membered macrolide generic structure as immunogen to construct, the antibody library theoretically can directly obtain a specific antibody library of 16-membered macrolide compound through screening, the screening flux is high, the efficiency is high, the specificity is strong, and the affinity selecting range is wide, so that the phage antibody library has wide application prospect in the aspects such as agricultural chemical antibody preparation, testing technique development and the like.

The invention discloses a paper base micro-fluidic chip for selecting soil active bacterium and components and application of the paper base micro-fluidic chip. A plurality of micro-fluidic units, and units, such as carbon paste three-electrode arrays for bacterial respiration electrochemical measurement are printed on paper to form two types of array dual paper base micro-chamber co-culture and activity screening function units, on chromatographic paper, a carbon paste electrode, a PDMS (Polydimethylsiloxane) passage, a PDMS micro-culture room and the like are sequentially overprinted through silk-screen printing, and furthermore, a model bacterial array unit is printed in an aseptic condition; a dual micro-chamber co-culture array unit of a sample bacterium-model bacterium and a secondary metabolite of a sample bacterium are used to inhibit adjacent model bacterium respiratory chains, and a carbon paste three-electrode printed together with a dual micro-chamber is used to find the position of some active sample bacterial colony; the secondary metabolite of the active sample bacterium is separated through paper base two-dimensional electrophoresis, and an array carbon paste three-electrode is used to find the positions of active components obtained through two-dimensional electrophoresis. The paper base micro-fluidic chip for selecting soil active bacterium is suitable for selecting the active bacterium and active complex components in multiple fields.

The invention provides high-yield coenzyme Q10 rhodobacter sphaeroides and mutation breeding and application thereof, and relates to the field of microbial mutation breeding. Strains screened by spacemutagenesis are used as starting bacteria and subjected to plasma mutagenesis at normal pressure and room temperature. The high-yield coenzyme Q10 rhodobacter sphaeroides are obtained after multi-resistance screening, high-throughput screening and step-by-step amplification verification, and are deposited in China General Microbiological Culture Collection Center with the deposit number of CGMCCNo. 16625. The average titer of a mutant strain of the rhodobacter sphaeroides can reach 2694[mu]/mL according to 20m<3> fermentation production verification, and the yield of the coenzyme Q10 is significantly improved. In addition, the strain has quadruple resistance and broad application prospects.

The invention discloses a multi-channel droplet quantitative measuring device based on pressure driving. The multi-channel droplet quantitative measuring device comprises a sample application probe for performing sample application operation on a sample solution, a working medium liquid storage tank for containing a working medium, a pressure control system for controlling the pressure in the working medium liquid storage tank, and a fluid damper provided with a micro-channel structure and having a damping effect on fluid in the micro-channel structure, wherein the liquid inlet end of the fluid damper communicates with the medium liquid storage tank, and the liquid outlet end of the fluid damper communicates with the liquid inlet end of the sample application probe in a sealed mode. The liquid droplet quantitative measuring device has the liquid droplet quantitative measuring capacity from picoliter level to microliter level and the liquid droplet quantitative measuring precision as low as picoliter level. According to the multi-channel droplet quantitative measuring device, various solutions with different viscosities can be measured in parallel, and the capability is an essentialcapability for large-scale high-throughput screening applications such as drug screening, protein crystallization condition screening and the like which need to treat a large amount of solutions withdifferent properties. The device is easy to construct, convenient to operate and good in reliability.

The invention discloses a method for analyzing epitope of a protein, comprising the following steps of: (1) constructing a deoxyribonucleic acid (DNA) library of a target protein; inserting the DNA segment of the coding gene of the protein into a T carrier, wherein the T carrier contains the coding gene of yeast a lectin Aga2p; fusing a peptide coded by the DNA segment with the C end of the yeast a lectin Aga2p; (2) displaying the DNA library on yeast surfaces to obtain a yeast display library; (3) separating out yeasts combined with the antibody of the target protein; and (4) extracting plasmid sequencing and analyzing the epitope of the protein. The invention has the advantages that (1) yeast cells have multiple modification paths on the protein, can conveniently display the complicated high molecular weight protein and better simulates a space conformation; (2) the yeasts can be screened one by one by adopting flow cytometry (FACS) so as to improve the accuracy and the screening flux; (3) the yeasts can independently complete the self growth reproduction process, the operation is simple and convenient and the interference factors are few; and (4) the yeasts are not easy to propagate in the air or generate cross infection.

The invention relates to a cell elution method based on drug target residence time. The method is characterized in that to-be-detected compounds are fully incubated with cells with drug targets expressed, the to-be-detected compounds dissociated from the drug targets are continuously removed by repeating the steps of elution, incubation and centrifugation three times, efficacy of the compounds notdissociated from the drug targets after elution is then detected by using a cell function experiment, an obtained result is compared with produced efficacy of to-be-detected compounds with the same concentration after full incubation with cells with drug targets expressed without elution, incubation and centrifugation, and residence time of the detected compounds is analyzed. The cell elution experiment method has the characteristics of being pollution-free, high in flux, simple to operate and the like.

The invention discloses an application of a high-throughput screening method using a droplet micro-fluidic chip in actinomycetes, actinomycete monospores can be embedded in a single droplet through the method, the single spores are stably formed in the droplet within the culture time of 0-7 days, the wide detection and sorting time is guaranteed, and in the aspect of detection, by using the method, functional promoters in actinomycetes can be detected at high throughput, or the strength of some unknown promoters can be identified, and promoter elements with proper strength can be screened for subsequent actinomycete strain modification; in the aspect of screening, the screening flux of the method can reach 105 strains per day, a mixed library of positive bacteria generating green fluorescence signals and negative bacteria not generating green fluorescence signals can be successfully sorted, and the enrichment rate of the sorted positive bacteria reaches 81.7% or above and is increased to 26 times compared with 3.1% before sorting.

The invention relates to the technical field of microorganisms, and particularly discloses saccharopolyspora spinosa DS190375 as well as a fermentation product, a microbial inoculum, a fermentation and screening method and an application of saccharopolyspora spinosa DS190375. The preservation number of the saccharopolyspora spinosa DS190375 disclosed by the invention is CGMCC No. 18681. The fermentation method comprises the following steps: rejuvenating and screening the strain in a high-salt plate culture medium, and fermenting. The invention also provides a screening method of the saccharopolyspora spinosa with high spinosad yield, which comprises the following steps: screening to obtain the saccharopolyspora spinosa with high salt tolerance, and screening the obtained saccharopolysporaspinosa with high salt tolerance to obtain the strain with high spinosad yield. The saccharopolyspora spinosa DS190375 disclosed by the invention is resistant to high salt and high in spinosad yield.The fermentation method avoids the problem of unstable spinosad yield caused by strain performance degradation.

The invention discloses a high-yielding penicillin strain, a screening method of the strain, and application of the strain in penicillin production, and belongs to the technical field of antibiotics production and application. The strain is characterized in that penicillin fermenting unit is increased by 13.2%, so that the yield of penicillin is effectively increased, and the production cost is decreased. The screening method comprises the following steps: (A) preparing a protoplast; (B) performing high-energy electronic mutagenesis; (C) rationally screening; and (D) screening with a shake bottle. The penicillin strain screening method is high in purposiveness, and high in mutation probability.

The invention provides a cell sorting device. The cell sorting device comprises a shell and a screening chip; the shell is internally provided with an inlet, a fluid passage and a first outlet, and the inlet and the first outlet are communicated with the fluid passage separately; the screening chip is arranged at the first outlet and comprises a screening area and a packaging area, the packaging area is located at the outer side of the screening area and used for being connected with the shell, a plurality of through holes are formed in the screening area, the screening area is made of a silicon material, and the thickness of the screening area is less than 25 micrometers. By means of the technical scheme, the problems of low throughput, complicated operation and easy breakage of cells inthe prior art can be solved.

The invention discloses a curved screen adjustable feeding system, and relates to the technical field of filtration treatment of wastewater. The curved screen adjustable feeding system comprises a pulp feeding pipe and a feeding device, wherein the width of a discharging hole of the feeding device is same as that of a screen plate of a curved screen; the discharging amount and the pressure of thedischarging hole are adjustable, and a water curtain of the discharging hole can be uniformly distributed on the screen plate of the curved screen, so that the utilization ratio of the screen plate ismaximized; meanwhile, pressure-type pulp feeding is realized, the screening capacity of equipment is obviously increased, and therefore, the screening efficiency of the equipment is effectively improved. In an operation process, according to the requirement of the screening capacity, the opening of the discharging hole is adjusted correspondingly, and the flow rate and pressure of pulp at the discharging hole can be adjusted; in addition, the opening of the discharging hole of the feeding device can be adjusted without stopping the equipment when in operation, so that the feeding system is wide in adjusting range, wide in application range and novel in structure, safe and reliable.

Disclosed are a 3-furyl-2-cyano-2-acrylamide derivative with epidermal growth factor receptor (EGFR) inhibitory activity and pharmaceutical acceptable salt thereof, together with preparation method thereof, pharmaceutical composition comprising the compound, and application of the compound in treating senile dementia (AD). The new compound is shown as formula I, wherein R₁ is selected from the group consisting of H, C₁-C₆ alkyl, C₂-C₆ alkenyl, C₂-C₆ alkynyl and C₃-C₆ cycloalkyl; R₂ is selected from the group consisting of alkyl, cycloalkyl, alkenyl, alkynyl, aryl, heteroaryl, aralkyl and heteroaralkyl; X is selected from the group consisting of CH₂, NH, O and S; m and n are all integers greater than or equal to zero.