8results about How to "Increase screening throughput" patented technology

The invention belongs to the technical field of strain screening, discloses a high-throughput screening method for sulfur-oxidizing bacteria and application, and particularly discloses application of 7-amino-4-methylcoumarin to screening of the sulfur-oxidizing bacteria or preparation of products for screening of the sulfur-oxidizing bacteria. The invention discloses the application of 7-amino-4-methylcoumarin in screening sulfur-oxidizing bacteria for the first time, and rapid, convenient, high-throughput and high-efficiency screening of sulfur-oxidizing bacteria can be realized through 7-amino-4-methylcoumarin.

This invention provides a label-free live cell screening system and method based on droplet microfluidics. The system includes: a microfluidic chip with fluorescence signal monitoring sites, imaging sites, and sorting sites; a fluorescence detection unit configured to excite and acquire fluorescence signals at the fluorescence signal monitoring sites; an imaging unit configured to acquire images of droplets at the imaging sites; a sorting unit configured to sort droplets encapsulating target cells at the sorting sites; and a signal processor that, when a droplet is detected passing through the fluorescence signal monitoring site based on the fluorescence signal, triggers the imaging unit to acquire images of the droplet at the imaging site; and when the signal processor determines, based on image analysis, that the droplet encapsulates a target cell, triggers the sorting unit to sort the droplet encapsulating the target cell at the sorting site. This invention utilizes fluorescence signals to monitor droplets, improving monitoring efficiency and accuracy, thereby significantly increasing the precision and throughput of droplet sorting.

A solid waste screening device and a screening system, wherein the screening device comprises a frame, a driving mechanism, a screening box and a collecting mechanism, the screening box has a blocking structure and a screening mesh extending obliquely relative to a first horizontal direction, the height of the feeding end of the screening mesh is lower than the height of the discharging end, the driving mechanism is used to drive the screening box to reciprocate along the first horizontal direction, and the blocking structure is used to limit the movement of the material in the screening box towards the feeding end. By adopting the low-in high-out structure with the feeding end lower than the discharging end, the material in the screening box can be gradually thrown towards the discharging end in a parabolic trajectory during the reciprocating movement of the screening box. When applied to the scene of multi-particle size classification screening, by differentiating the aperture of the screening mesh along the extension direction or by serially combining multiple screening boxes or multiple screening devices along the first horizontal direction, not only can the multi-particle size step-by-step classification screening be realized, but also the structural compactness of the device or system can be enhanced and the occupation of vertical space can be significantly reduced.

The invention discloses a cell chip for evaluating skin discomfort caused by a product and application of the cell chip. The cell chip comprises a detection hole of an overexpression cell containing a sensory receptor gene, the overexpression cells of the sensory receptor gene comprise various overexpression cells of a TRPs receptor family, a histamine receptor family, a protease activated receptor family and a prostaglandin receptor family. According to the invention, a plurality of key sensory receptors participating in skin discomfort regulation are adopted as comprehensive evaluation indexes, and a stable cell strain constructed in an overexpression mode is adopted, so that the skin sensory receptors with high expression quantity under abnormal sensitive perception of skin can be simulated; then, the change multiple of the in-vitro intracellular calcium ion fluorescence signal intensity is used for calculating and relating to the evaluation of clinical skin discomfort through a specific score, so that the evaluation of an in-vitro cell chip can be used as a prediction model for the evaluation of the clinical skin discomfort; and an innovative and accurate in-vitro cytology method capable of replacing clinic is provided for evaluating the skin discomfort.

This invention provides a multi-to-multi drug screening method for CRISPRa / i GPCR libraries based on a three-dimensional spatial hybrid screening model and its applications, belonging to the field of biomedical technology. The method includes the following steps: Step (1): Constructing a screening cell pool; Step (2): Classifying the drugs to be screened; Step (3): Constructing a drug hybrid screening model; Step (4): Grouping drugs according to mutually perpendicular X, Y, and Z planes, with drugs in each plane mixed to form independent pools; Step (5): Processing each pool into a cell pool; Step (6): Detecting and analyzing the changes in sgRNA abundance in each pool; Step (7): Decoding to determine the drug-GPCR interaction pairs. This invention offers higher screening throughput, realizes a multi-target, multi-drug screening mode, reduces costs and time, and enables large-scale research on drug-gene interaction networks, which is of great significance for drug research and development.

The application belongs to the technical field of bioengineering, and discloses a screening platform for optimizing the interval of baculovirus bidirectional promoters, a screening method and application. The application constructs a high-resolution screening platform with a reference gene integrated in a virus skeleton, and through two-stage screening, first, a transcription enhancement interval is determined by rough screening in the 98-245bp interval, and then, fine screening is performed in the transcription enhancement interval by 1bp steps, so that the optimal interval length of the p10 and polh promoters is determined as 153bp by normalizing the reference fluorescent signal to correct errors such as infection efficiency. The interval is applied to a recombinant adeno-associated virus (rAAV) packaging system, transcription interference between the bidirectional promoters is eliminated, the expression balance of Rep and Cap proteins is restored, the rAAV genome replication efficiency is improved by 3 times, the empty shell rate is reduced from 84.9% to 20.1%, and the transduction efficiency in mammalian cells can be maintained.

The invention discloses a method for rapidly detecting polymyxin B fermentation liquor, which belongs to the field of microorganisms and comprises the following steps: taking paenibacillus polymyxa, and detecting the fermentation liquor through activation, plate single colony separation, 48-pore plate culture and 96-pore plate biological turbidimetry. According to the method disclosed by the invention, a 48-pore plate is used for replacing a shake flask to culture a single colony obtained by flat plate separation, and meanwhile, a biological turbidimetric method is used for replacing liquid phase detection, so that the consumption of manpower and material resources is reduced, the single screening sample amount is increased, and the screening and detection efficiency is improved.

The utility model relates to the technical field of vibrating screens, in particular to a premixed powder vibrating screen which comprises a vibrating screen body, a cover plate is arranged at the upper end of the vibrating screen body, a feeding hopper is rotatably installed in the cover plate, a feeding structure is fixedly installed on the circumferential surface of the feeding hopper and comprises a first gear, and a servo motor is fixedly installed in the cover plate. By arranging a feeding structure, when a worker discharges premixed flour through a feeding hopper, the worker can start a servo motor firstly, so that an output shaft of the servo motor drives a second gear to rotate to be meshed with a first gear, and the first gear drives the feeding hopper to rotate; the feeding hopper drives the inclined pipeline, the rotating joint and the discharging pipe to discharge materials between the vibrating screen body and the cover plate in a rotating mode, so that the regional screening treatment capacity in unit time is improved, premixed powder can pass through the screen more quickly, the screening time is shortened, and the efficiency of the whole production process is improved.