5results about How to "Increase screening throughput" patented technology

This invention provides a label-free live cell screening system and method based on droplet microfluidics. The system includes: a microfluidic chip with fluorescence signal monitoring sites, imaging sites, and sorting sites; a fluorescence detection unit configured to excite and acquire fluorescence signals at the fluorescence signal monitoring sites; an imaging unit configured to acquire images of droplets at the imaging sites; a sorting unit configured to sort droplets encapsulating target cells at the sorting sites; and a signal processor that, when a droplet is detected passing through the fluorescence signal monitoring site based on the fluorescence signal, triggers the imaging unit to acquire images of the droplet at the imaging site; and when the signal processor determines, based on image analysis, that the droplet encapsulates a target cell, triggers the sorting unit to sort the droplet encapsulating the target cell at the sorting site. This invention utilizes fluorescence signals to monitor droplets, improving monitoring efficiency and accuracy, thereby significantly increasing the precision and throughput of droplet sorting.

A solid waste screening device and a screening system, wherein the screening device comprises a frame, a driving mechanism, a screening box and a collecting mechanism, the screening box has a blocking structure and a screening mesh extending obliquely relative to a first horizontal direction, the height of the feeding end of the screening mesh is lower than the height of the discharging end, the driving mechanism is used to drive the screening box to reciprocate along the first horizontal direction, and the blocking structure is used to limit the movement of the material in the screening box towards the feeding end. By adopting the low-in high-out structure with the feeding end lower than the discharging end, the material in the screening box can be gradually thrown towards the discharging end in a parabolic trajectory during the reciprocating movement of the screening box. When applied to the scene of multi-particle size classification screening, by differentiating the aperture of the screening mesh along the extension direction or by serially combining multiple screening boxes or multiple screening devices along the first horizontal direction, not only can the multi-particle size step-by-step classification screening be realized, but also the structural compactness of the device or system can be enhanced and the occupation of vertical space can be significantly reduced.

This invention provides a multi-to-multi drug screening method for CRISPRa / i GPCR libraries based on a three-dimensional spatial hybrid screening model and its applications, belonging to the field of biomedical technology. The method includes the following steps: Step (1): Constructing a screening cell pool; Step (2): Classifying the drugs to be screened; Step (3): Constructing a drug hybrid screening model; Step (4): Grouping drugs according to mutually perpendicular X, Y, and Z planes, with drugs in each plane mixed to form independent pools; Step (5): Processing each pool into a cell pool; Step (6): Detecting and analyzing the changes in sgRNA abundance in each pool; Step (7): Decoding to determine the drug-GPCR interaction pairs. This invention offers higher screening throughput, realizes a multi-target, multi-drug screening mode, reduces costs and time, and enables large-scale research on drug-gene interaction networks, which is of great significance for drug research and development.

The application belongs to the technical field of bioengineering, and discloses a screening platform for optimizing the interval of baculovirus bidirectional promoters, a screening method and application. The application constructs a high-resolution screening platform with a reference gene integrated in a virus skeleton, and through two-stage screening, first, a transcription enhancement interval is determined by rough screening in the 98-245bp interval, and then, fine screening is performed in the transcription enhancement interval by 1bp steps, so that the optimal interval length of the p10 and polh promoters is determined as 153bp by normalizing the reference fluorescent signal to correct errors such as infection efficiency. The interval is applied to a recombinant adeno-associated virus (rAAV) packaging system, transcription interference between the bidirectional promoters is eliminated, the expression balance of Rep and Cap proteins is restored, the rAAV genome replication efficiency is improved by 3 times, the empty shell rate is reduced from 84.9% to 20.1%, and the transduction efficiency in mammalian cells can be maintained.

The utility model relates to the technical field of vibrating screens, in particular to a premixed powder vibrating screen which comprises a vibrating screen body, a cover plate is arranged at the upper end of the vibrating screen body, a feeding hopper is rotatably installed in the cover plate, a feeding structure is fixedly installed on the circumferential surface of the feeding hopper and comprises a first gear, and a servo motor is fixedly installed in the cover plate. By arranging a feeding structure, when a worker discharges premixed flour through a feeding hopper, the worker can start a servo motor firstly, so that an output shaft of the servo motor drives a second gear to rotate to be meshed with a first gear, and the first gear drives the feeding hopper to rotate; the feeding hopper drives the inclined pipeline, the rotating joint and the discharging pipe to discharge materials between the vibrating screen body and the cover plate in a rotating mode, so that the regional screening treatment capacity in unit time is improved, premixed powder can pass through the screen more quickly, the screening time is shortened, and the efficiency of the whole production process is improved.