Ampere enzyme electrode based on electrostatic spinning fiber membrane and method for making same

Abstract

The invention discloses an amperometric enzyme electrode based on an electrostatic spinning fibrous membrane and a method for preparing the same. The method uses dimethyl formamide as solvent, a platinum electrode, a gold electrode, a glassy carbon electrode or a carbon paste electrode as a receiving electrode and acrylonitrile copolymer as raw materials, and deposits a copolymer fibrous membrane layer on the surface of the electrode through the electrostatic spinning method. The method is as follows: firstly, a monolayer of redoxase is constructed on the surface of fibers through the covalent method; secondly, bifunctional reagent is added into enzyme solution, and enzyme congeries is driven to be covalently fixed on the enzyme membrane on the surface of the electrode. In the amperometric enzyme electrode, due to large specific surface area and high porosity of the fibrous membrane and the unique enzyme immobilization method, the electrochemical enzyme electrode is characterized by large response strength, high stability, wide linear detection range and so on. The method for preparing the enzyme electrode is simple and is suitable for mass production, and the enzyme electrode can be reused and preserved for a long term.

Description

Technical field

[0001] The invention relates to an ampere enzyme electrode and a preparation method, in particular to an ampere enzyme electrode based on an electrospun fiber membrane and a preparation method thereof. Background technique

[0002] The biosensor is mainly composed of two parts, namely the biosensing membrane (also known as the molecular recognition element) and the transducer. The analyte diffuses into the bio-sensitive membrane. After molecular recognition and biochemical reaction, the generated information is converted into a quantitatively processed electrical signal by the corresponding physical or chemical transducer, and then amplified and output by the detection instrument. You can know the concentration of the analyte. Among them, the biosensitive membrane is the key part of the biosensor, it is the basis for the selective detection of the biosensor, and determines the function and quality of the sensor. The biological components contained in it can be enz...