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Ampere enzyme electrode based on electrostatic spinning fiber membrane and method for making same

A fiber membrane and enzyme electrode technology, which is applied in the field of electrospinning fiber membrane-based amperometric electrode and its preparation, can solve the problems of narrow linear detection range, low stability, low electrode response, etc., and widen the linear detection range. , the effect of high enzyme loading efficiency and enzyme activity, and simple preparation method

Inactive Publication Date: 2008-10-22
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide an amperometric enzyme electrode based on electrospun fiber membrane and its preparation method in view of the shortcomings of traditional immobilized enzyme electrodes such as low response, narrow linear detection range and low stability.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1) Dissolve acrylonitrile copolymer (viscosity average molecular weight of 100,000, comonomer mole percentage of 5%) in dimethylformamide to prepare an acrylonitrile copolymer solution with a mass fraction of 3%; use platinum electrode as The receiving plate, when the voltage is 10 kV, the extrusion rate is 0.5 ml / h, and the receiving distance is 10 cm, the fiber membrane is collected, and the spinning time lasts for 2 hours to obtain the electrode deposited with the nano-fiber membrane;

[0027] 2) Immerse the surface of the electrode deposited with the nanofiber membrane in the buffer solution of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide, where 1 The concentration of -(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride is 5 mg / ml, and the concentration of N-hydroxysuccinimide is 3 mg / ml, shake at 25°C Activate for 2 hours, then wash with buffer solution 3 times; immerse the activated electrode surface in a buffer solution c...

Embodiment 2

[0029] 1) Dissolve acrylonitrile copolymer (viscosity average molecular weight of 30,000, comonomer mole percentage of 20%) in dimethylformamide to prepare an acrylonitrile copolymer solution with a mass fraction of 10%; take gold electrode as The receiving plate, when the voltage is 20 kV, the extrusion rate is 3.0 ml / h, and the receiving distance is 20 cm, the fiber membrane is collected, and the spinning time lasts for 10 hours to obtain the electrode deposited with the nano-fiber membrane;

[0030]2) Immerse the surface of the electrode deposited with the nanofiber membrane in a buffer solution of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide, where 1 The concentration of -(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride is 20 mg / ml, and the concentration of N-hydroxysuccinimide is 12 mg / ml, shake at 25°C Activated for 10 hours, and then washed with buffer solution 5 times; immerse the activated electrode surface in a buffer solu...

Embodiment 3

[0032] 1) Dissolve acrylonitrile copolymer (viscosity average molecular weight of 30,000, comonomer mole percentage of 20%) in dimethylformamide to prepare an acrylonitrile copolymer solution with a mass fraction of 10%; use glassy carbon electrode For the receiving plate, when the voltage is 20 kV, the extrusion rate is 3.0 ml / h, and the receiving distance is 20 cm, the fiber membrane is collected, and the spinning time lasts for 10 hours to obtain the electrode deposited with the nano-fiber membrane;

[0033] 2) Immerse the surface of the electrode deposited with the nanofiber membrane in a buffer solution of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide, where 1 The concentration of -(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride is 20 mg / ml, and the concentration of N-hydroxysuccinimide is 12 mg / ml, shake at 4°C Activated for 10 hours, and then washed with buffer solution 5 times; immerse the activated electrode surface in a bu...

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Abstract

The invention discloses an amperometric enzyme electrode based on an electrostatic spinning fibrous membrane and a method for preparing the same. The method uses dimethyl formamide as solvent, a platinum electrode, a gold electrode, a glassy carbon electrode or a carbon paste electrode as a receiving electrode and acrylonitrile copolymer as raw materials, and deposits a copolymer fibrous membrane layer on the surface of the electrode through the electrostatic spinning method. The method is as follows: firstly, a monolayer of redoxase is constructed on the surface of fibers through the covalent method; secondly, bifunctional reagent is added into enzyme solution, and enzyme congeries is driven to be covalently fixed on the enzyme membrane on the surface of the electrode. In the amperometric enzyme electrode, due to large specific surface area and high porosity of the fibrous membrane and the unique enzyme immobilization method, the electrochemical enzyme electrode is characterized by large response strength, high stability, wide linear detection range and so on. The method for preparing the enzyme electrode is simple and is suitable for mass production, and the enzyme electrode can be reused and preserved for a long term.

Description

Technical field [0001] The invention relates to an ampere enzyme electrode and a preparation method, in particular to an ampere enzyme electrode based on an electrospun fiber membrane and a preparation method thereof. Background technique [0002] The biosensor is mainly composed of two parts, namely the biosensing membrane (also known as the molecular recognition element) and the transducer. The analyte diffuses into the bio-sensitive membrane. After molecular recognition and biochemical reaction, the generated information is converted into a quantitatively processed electrical signal by the corresponding physical or chemical transducer, and then amplified and output by the detection instrument. You can know the concentration of the analyte. Among them, the biosensitive membrane is the key part of the biosensor, it is the basis for the selective detection of the biosensor, and determines the function and quality of the sensor. The biological components contained in it can be enz...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
Inventor 徐志康王振刚黄小军
Owner ZHEJIANG UNIV
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