The invention belongs to the field of biological
catalysis, and particularly relates to a novel
immobilized enzyme preparation, and a preparation method and application thereof. The preparation method of the novel
immobilized enzyme preparation comprises the following steps: combining nano magnetic
chitosan microspheres and a
metal-organic framework material having so many properties such as multiple pores, large specific area, multiple
metal sites and the like through graft copolymerization to obtain a nano magnetic
chitosan-
metal organic framework material having a core-shell structure with a
porous network structure; and mixing the nano magnetic
chitosan-
metal organic framework material used as a carrier and an
enzyme solution, and immobilizing at 0-25 DEG C for 1-12 hours to obtain the novel
immobilized enzyme preparation. The method can immobilize enzymes such as
lipase, proteinase,
peroxidase and the like, and has the advantages of low cost, high immobilization efficiency, favorable
enzyme stability, high
enzyme loading amount and high enzyme
recovery rate; no
bifunctional reagent is used in the immobilization process; and the method is simple to operate, low in cost and mild in reaction.