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Target response type hydrogel and smart phone integrated on-site quantitative organophosphorus pesticide detection platform

A technology of organophosphorus pesticides and smartphones, applied in the field of biosensors, can solve the problems of pesticide concentration requirements, low detection sensitivity, cumbersome test methods, etc., and achieve the effects of fast response, simple construction process, and low cost

Active Publication Date: 2020-05-22
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a target-responsive hydrogel based on overcoming the problems existing in the prior art, such as long test time, large error, high requirements for pesticide concentration, cumbersome test methods, and low detection sensitivity. On-site quantitative detection platform for organophosphorus pesticides integrated with smartphones (referred to as: kit-smartphone platform), which can detect the activity of organophosphorus pesticides simply, quickly and with high sensitivity

Method used

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  • Target response type hydrogel and smart phone integrated on-site quantitative organophosphorus pesticide detection platform
  • Target response type hydrogel and smart phone integrated on-site quantitative organophosphorus pesticide detection platform
  • Target response type hydrogel and smart phone integrated on-site quantitative organophosphorus pesticide detection platform

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: MnO 2 Synthesis of NFs hydrogels

[0030] First add 0.5mg mL -1 BSA with 5.0 mL MnCl 2 (4.0×10 -3 mol L -1 ) aqueous solution was mixed, and the mixture solution was continuously stirred at room temperature for 1 hour. Subsequently, with 1.0mol L -1 NaOH solution adjusted the pH of the reaction mixture to 10.0. After the reaction, it was allowed to stand for 4 hours, and the product was stored at 4.0°C. Then, fully dissolve SA in Tris-HCl buffer pH 8.0, quickly transfer SA (20mg mL -1 ) into a 96-well plate. Next add 0.025mg mL -1 MnO 2 NFs were added to SA, and immediately after mixing well, CaCl was added dropwise to the above mixture 2 (5.0mg mL -1 ), made of uniform BSA-MnO 2 NFs-SA hybrid hydrogel;

Embodiment 2

[0031] Embodiment 2: the making of kit

[0032] 3.0 μg mL -1 Paraoxon aqueous solution (20μL) and acetylcholinesterase (8.0U mL -1 ) aqueous solution (20 μL) was mixed and incubated at 37° C. for 20 minutes. Then add 40 μL acetylcholine (100 mmol L -1 ) and choline oxidase (4.0U mL -1 ) aqueous solution. After incubating at 37°C for 30 minutes, the reaction solution was added to the BSA-MnO obtained in Example 1 2 NFs-SA mixed hydrogel, thereby obtaining the MnO described in the present invention 2 NFs Hydrogel Kit. Add TMB (1.5mmol L -1 , 20 μL) chromogenic reagent, the color reaction of the kit is triggered. At the concentration of paraoxon in this example, the color of the kit is dark blue with a gray value of 79.405.

Embodiment 3

[0033] Embodiment 3: the application of kit to OPs detection

[0034] According to the method of Example 2, different concentrations of OPs (0, 0.0005, 0.005, 0.05, 0.3, 0.5, 1.5 and 3.0 μg mL -1 , the corresponding gray values ​​at different concentrations are 159.07, 119.81, 109.33, 90.95, 84.79, 82.36, 81.42, 79.405) MnO 2 NFs hydrogel kit, and then drop 20μL, 1.5mmol L -1 After TMB chromogen, the color of the kit can change from colorless to dark blue as the concentration of OPs increases with the naked eye, and the color change is recorded using an application (APP) made by a smart phone. It has a built-in image processing program, which can convert the corresponding image information into gray value, and further display the linear relationship with the logarithm of paraoxon concentration, so as to realize the on-site handheld detection of OPs (such as figure 2 shown);

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Abstract

The invention discloses an on-site quantitative organophosphorus pesticide detection platform based on integration of target response type hydrogel and a smart phone, and belongs to the technical field of biosensors. A hydrogel material with target response serves as a carrier, has MnO2NFs similar to oxidase activity, can oxidize a colorless 3, 3', 5-, 5'-tetramethylbenzidine color developing agent into blue oxTMB, and can decompose MnO2NFs in the presence of H2O2 to lose enzyme-like activity. The organophosphorus pesticide (paraoxon) serving as an inhibitor of AChE can inhibit AChE from catalyzing acetylcholine to generate H2O2 and reduce decomposition of MnO2NFs, so that color response of a kit is stimulated, and collection and analysis of image data of the kit are further realized in combination with the smart phone. The OPs can be rapidly detected in a handheld mode on site, the method has the advantages of being easy and convenient to operate, high in sensitivity, low in cost andthe like, and a new perspective is provided for portable monitoring of food safety and human health.

Description

technical field [0001] The invention belongs to the technical field of biosensors, and in particular relates to an on-site quantitative detection platform for organophosphorus pesticides based on the integration of a target-responsive hydrogel and a smart phone. Background technique [0002] Organophosphorus pesticides (OPs) are necessary substances to ensure high crop yields. According to previous data surveys, the use of pesticides can ensure an increase of one-third of the global crop yield. However, the abuse and improper treatment of OPs make them infiltrate into soil, atmospheric circulation and water circulation, resulting in toxic pesticide residues in crops such as fruits and vegetables, further posing a serious threat to ecosystems and even human health. Accurate tracking of OPs in recent years has become one of the most worrying challenges to establish proper management of pesticides, protect food resources, and reduce OPs poisoning. Therefore, methods for accura...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/01G01N1/38
CPCG01N1/38G01N21/01G01N21/78
Inventor 卢革宇闫旭金蕊孙鹏刘方猛王晨光刘晓敏高原梁喜双刘凤敏
Owner JILIN UNIV
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