The invention provides a pH color-response sensing type micro-nanofiber, a preparation method and application thereof, which relate to a response sensing type micro-nanofiber, a preparation method and application thereof. The invention solves the problems of low sensitivity and long reaction time of the traditional poly-Schiff base film for acidichromism. The diameter of the micro-nanofiber is between 10 nanometers and 2 micrometers, and the micro-nanofiber is prepared from a spinning solution through an electrostatic spinning technology. The preparation method comprises the following steps of: firstly, dissolving the poly-Schiff base into a polymer solution to prepare the spinning solution, and then, preparing the micro-nanofiber by the electrostatic spinning technology. The micro-nanofiber is applied to a pH color response sensor, is evenly distributed, is in bunched fiber or a felty film and has the advantages of sensitive pH color response and short metachromatism time (0.5s-1s). The pH color-response sensing type micro-nanofiber has obvious color response for 0.1-10000ppm response matters. The preparation method is completed at room temperature, has the advantages of short preparation time, convenient operation and low energy consumption, and is convenient to practice.