A ce-n-c nanozyme and its application in the detection of organophosphorus pesticide residues

Abstract

The invention belongs to the technical field of pesticide residue detection, and discloses a Ce-N-C nanozyme and its application in the detection of organophosphorus pesticide residues. Described Ce-N-C nanometer enzyme has imitative hydrolase activity; And the synthetic method of described Ce-N-C nanometer enzyme is to take glucose and dicyandiamine as precursors, add Ce(SO4) 4 ) 2 ·4H 2 O reacts to obtain a reaction product, and the reaction product is pyrolyzed at high temperature to prepare a Ce-N-C nanozyme; the Ce-N-C nanozyme is applied to the detection of organophosphorus pesticide paraoxon. The Ce-N-C nanozyme preparation method is simple, and can complete the hydrolysis and rapid detection of the organophosphorus pesticide paraoxon by Ce-N-C.

Description

technical field

[0001] The invention belongs to the technical field of pesticide residue detection, in particular to a Ce-N-C nanozyme and its application in the detection of organophosphorus pesticide residues. Background technique

[0002] Organophosphorus pesticides have been playing an important role in crop production since their inception, playing an important role in controlling pests and diseases, removing weeds and regulating crop growth. With the continuous improvement of people's living standards in my country, the quality and safety of agricultural products has attracted more and more attention, especially the problem of pesticide residues in vegetables and fruits has become the focus of public attention. Pesticide residues will not only pollute the environment, but also enter the human body through various ways, blocking the transmission of neurotransmitters, leading to dysfunction of the nervous system, causing physical discomfort and even death. Acute poisoni...

Examples

Embodiment 1

[0029] Example 1 Synthesis of Ce-N-C Nanozyme

[0030] (a) Weigh 3g glucose (Glucose), 8g dicyandiamide (DICY) in a 100mL beaker, then add 3mmol / L Ce (SO 4 ) 2 ·4H2 The O solution was stirred at room temperature for 10 h to obtain a homogeneous solution product. The obtained product was centrifuged and dried under vacuum to obtain a white powder.

[0031] (b) Weighing the white powder obtained in step (a) and placing it in a clean quartz boat, and placing the quartz boat in a tube furnace, in N 2 Under the protection of the atmosphere, heated to the target reaction temperature of 800 °C at a heating rate of 4 °C / min, and continued to keep the temperature for 2.5 h; during the cooling process, continue to feed N 2 ; After the tube furnace is naturally cooled to room temperature, the black powder in the quartz boat is collected, which is Ce-N-C nanozyme; the obtained Ce-N-C nanozyme is observed by transmission electron microscope, and the figure 1 The TEM image of the Ce-N-C ...

Embodiment 2

[0032] Example 2 Synthesis of Ce-N-C Nanozyme

[0033] (a) Weigh 1 g of glucose (Glucose) and 5 g of dicyandiamide (DICY) into a 100 mL beaker, then add 1 mmol / L Ce (SO 4 ) 2 ·4H 2 The O solution was stirred at room temperature for 8 h, and the obtained product was centrifuged and dried in vacuo to obtain a white powder.

[0034] (b) Weighing the white powder obtained in step (a) and placing it in a clean quartz boat, and placing the quartz boat in a tube furnace, in N 2 Under the protection of the atmosphere, heated to the target reaction temperature of 700 °C at a heating rate of 3 °C / min, and continued to hold for 2 h; during the cooling process, continue to feed N 2 ; After the tube furnace is naturally cooled to room temperature, the black powder in the quartz boat is collected, which is the Ce-N-C nanozyme.

Embodiment 3

[0035] Example 3 Synthesis of Ce-N-C Nanozyme

[0036] (a) Weigh 5g glucose (Glucose), 10g dicyandiamide (DICY) in a 100mL beaker, then add 5mmol / L Ce (SO 4 ) 2 ·4H 2 The O solution was stirred at room temperature for 12 h, and the obtained product was centrifuged and dried in vacuo to obtain a white powder.

[0037] (b) Weighing the white powder obtained in step (a) and placing it in a clean quartz boat, and placing the quartz boat in a tube furnace, in N 2 Under the protection of the atmosphere, heated to the target reaction temperature of 950 °C at a heating rate of 5 °C / min, and continued to hold for 3 h; during the cooling process, continue to feed N 2 ; After the tube furnace is naturally cooled to room temperature, the black powder in the quartz boat is collected, which is the Ce-N-C nanozyme.

technical field

[0001] The invention belongs to the technical field of pesticide residue detection, in particular to a Ce-N-C nanozyme and its application in the detection of organophosphorus pesticide residues. Background technique

[0002] Organophosphorus pesticides have been playing an important role in crop production since their inception, playing an important role in controlling pests and diseases, removing weeds and regulating crop growth. With the continuous improvement of people's living standards in my country, the quality and safety of agricultural products has attracted more and more attention, especially the problem of pesticide residues in vegetables and fruits has become the focus of public attention. Pesticide residues will not only pollute the environment, but also enter the human body through various ways, blocking the transmission of neurotransmitters, leading to dysfunction of the nervous system, causing physical discomfort and even death. Acute poisoni...

Examples

Embodiment 1

[0029] Example 1 Synthesis of Ce-N-C Nanozyme

[0030] (a) Weigh 3g glucose (Glucose), 8g dicyandiamide (DICY) in a 100mL beaker, then add 3mmol / L Ce (SO 4 ) 2 ·4H2 The O solution was stirred at room temperature for 10 h to obtain a homogeneous solution product. The obtained product was centrifuged and dried under vacuum to obtain a white powder.

[0031] (b) Weighing the white powder obtained in step (a) and placing it in a clean quartz boat, and placing the quartz boat in a tube furnace, in N 2 Under the protection of the atmosphere, heated to the target reaction temperature of 800 °C at a heating rate of 4 °C / min, and continued to keep the temperature for 2.5 h; during the cooling process, continue to feed N 2 ; After the tube furnace is naturally cooled to room temperature, the black powder in the quartz boat is collected, which is Ce-N-C nanozyme; the obtained Ce-N-C nanozyme is observed by transmission electron microscope, and the figure 1 The TEM image of the Ce-N-C ...

Embodiment 2

[0032] Example 2 Synthesis of Ce-N-C Nanozyme

[0033] (a) Weigh 1 g of glucose (Glucose) and 5 g of dicyandiamide (DICY) into a 100 mL beaker, then add 1 mmol / L Ce (SO 4 ) 2 ·4H 2 The O solution was stirred at room temperature for 8 h, and the obtained product was centrifuged and dried in vacuo to obtain a white powder.

[0034] (b) Weighing the white powder obtained in step (a) and placing it in a clean quartz boat, and placing the quartz boat in a tube furnace, in N 2 Under the protection of the atmosphere, heated to the target reaction temperature of 700 °C at a heating rate of 3 °C / min, and continued to hold for 2 h; during the cooling process, continue to feed N 2 ; After the tube furnace is naturally cooled to room temperature, the black powder in the quartz boat is collected, which is the Ce-N-C nanozyme.

Embodiment 3

[0035] Example 3 Synthesis of Ce-N-C Nanozyme

[0036] (a) Weigh 5g glucose (Glucose), 10g dicyandiamide (DICY) in a 100mL beaker, then add 5mmol / L Ce (SO 4 ) 2 ·4H 2 The O solution was stirred at room temperature for 12 h, and the obtained product was centrifuged and dried in vacuo to obtain a white powder.

[0037] (b) Weighing the white powder obtained in step (a) and placing it in a clean quartz boat, and placing the quartz boat in a tube furnace, in N 2 Under the protection of the atmosphere, heated to the target reaction temperature of 950 °C at a heating rate of 5 °C / min, and continued to hold for 3 h; during the cooling process, continue to feed N 2 ; After the tube furnace is naturally cooled to room temperature, the black powder in the quartz boat is collected, which is the Ce-N-C nanozyme.

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