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Organophosphorus anhydrase, coding gene thereof and application of organophosphorus anhydrase in degradation of organophosphorus pesticides

A technology of organophosphoric anhydrase and organophosphorus pesticides, which can be used in applications, genetic engineering, plant gene improvement, etc., and can solve problems such as difficulties in protein recombination and expression

Inactive Publication Date: 2021-04-16
深圳中科欣扬生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported in the literature that PTE is mainly involved in the transportation of phosphate substances as a lipoprotein linked to the inner membrane of the cell, so the recombinant expression of its protein is difficult, and most of the cases exist as inactive inclusion bodies

Method used

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  • Organophosphorus anhydrase, coding gene thereof and application of organophosphorus anhydrase in degradation of organophosphorus pesticides
  • Organophosphorus anhydrase, coding gene thereof and application of organophosphorus anhydrase in degradation of organophosphorus pesticides
  • Organophosphorus anhydrase, coding gene thereof and application of organophosphorus anhydrase in degradation of organophosphorus pesticides

Examples

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Embodiment 1

[0075]Example 1, expression and activity assay of OPAA1436951 protein

[0076]First, OPAA1436951 protein encoding gene is obtained

[0077]The organic phosphatase base from Alteromonas, which reported in the literature, by template, from 29 viable Indian Ocean deposition samples, high-throughput sequencing data, by homogenous sequence, to obtain OPAA1436951 gene, hereinafter referred to as OPAA1 (DNA molecules shown in Sequence 1 in the sequence 1 in the sequence table, CDS are DNA molecules shown in Sequence 1). The gene encoded protein is named OPAA 1436951 (hereinafter referred to as OPAA1), which is shown in the amino acid sequence, such as sequence 2 in the sequence 2, is shown in Sequence 2.

[0078]At the same time, another OPAA2289482 gene that is simultaneously screened is introduced, and the following referred to as OPAA2 (DNA molecules shown in Sequence 3 in the sequence 3, CDS are the 4-1320-bit DNA molecules shown in sequence 3). The gene encoded protein is named OPAA2289482 (he...

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Abstract

The invention discloses organophosphorus anhydrase as well as a coding gene and application thereof. The recombinant organophosphorus anhydrase to be protected in the invention is recombinant organophosphorus anhydrase OPAA1 or OPAA2. The amino acid sequence of the OPAA1 is a protein of a sequence 2 in a sequence table or a protein having more than 80% of identity with the protein of the sequence 2; and the amino acid sequence of the OPAA2 is a protein shown as a sequence 4 in a sequence table or a protein having more than 80% of identity with the protein shown as the sequence 4. The recombinant organophosphorus anhydrase prepared and expressed by the invention is soluble and easy to purify, and can degrade dipeptide with proline at the C end, organophosphorus pesticide paraoxon, especially phosphorus S and malathion absorbed by PS-containing organophosphorus pesticide, which are not reported before. The OPAA1 has a wide application prospect in the aspects of organophosphorus pesticide degradation and ecological restoration and in the aspect of green decontamination.

Description

Technical field[0001]The present invention belongs to the biotechnology, and in particular to an organophosphatase and its encoding gene and its application in organophosphorus pesticide degradation.Background technique[0002]Organophosphates, Ops is artificially synthetic hypervisible substances, mainly including organophosphorus pesticides and chemical warrants, are widely used in agriculture, firemakers, plasticizers, chemical weapons and other agriculture, industrial and national defense field. However, due to its high accumulation, high residue, high toxicity and fast function, the public health safety and ecosystem have caused serious damage. The organophosphorus compound can inhibit its activity in an irreversible binding of acetylcholine esterase in insects and mammals, so that the acetylcholine accumulation in the body causes nervous system disorders or even death.[0003]The method of currently degrading organophosphorus compounds mainly includes three physical methods, chemi...

Claims

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Application Information

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IPC IPC(8): C12N9/16C12N15/55C12N15/70C12N15/62C12N1/21A62D3/02C12R1/19A62D101/04A62D101/26A62D101/28
Inventor 董亮郑晓芳张山
Owner 深圳中科欣扬生物科技有限公司
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