Mutant of organophosphorus hydrolase, expression vector, recombinant bacterium and application of recombinant bacterium

An expression vector and mutant technology, applied in the fields of genetic engineering and enzymology, can solve the problems of inability to meet the requirements of fast and efficient, fast degradation of organophosphorus pesticides, narrow applicable environment, insufficient secretion and expression of enzymes, etc.
CN110862974AInactive Publication Date: 2020-03-06KARAMAY SANDA NEW TECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
KARAMAY SANDA NEW TECH
Publication Date
2020-03-06
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a mutant of an organophosphorus hydrolase, an expression vector, a recombinant bacterium and an application of the recombinant bacterium, and belongs to the technical field of genetic engineering and enzymology. According to the mutant, isoleucine at a 274th position of organophosphorus hydrolase mutates into asparagin. Compared with organophosphorus hydrolase, the providedmutant of the organophosphorus hydrolase improves the hydrolysis rate of paraoxon compounds.
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Description

technical field

[0001] The invention belongs to the technical fields of genetic engineering and enzymology, and in particular relates to a mutant, an expression vector, a recombinant bacterium and an application of an organophosphate hydrolase. Background technique

[0002] Organophosphate hydrolase (Organophosphorus hydrolase, OPH) is widely used in the biodegradation of organophosphorus pesticide residues, which is of great significance to the restoration of environmental pollution and the protection of human health. Organophosphate hydrolase OPH is a typical phosphotriesterase, which dissociates various phosphate bonds (P-O bond, P-CN bond, P-F bond and P- S bond), thus hydrolyzing many organophosphate triesters, thioesters, and fluorophosphoric esters. There are three hydrophobic pockets in the active center of OPH enzyme, which respectively interact with the three substituents of the substrate molecule to affect the release rate of the leaving group and determine the s...

Claims

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