The invention provides a Brazilian green propolis quality control method. The Brazilian green propolis quality control method is implemented through the following steps that a test propolis sample is ultrasonically extracted with ethyl alcohol, filtered and detected through high performance liquid chromatography, the content of six evaluation index ingredients is calculated according to a standard curve, and whether the quality of Brazilian green propolis is high or low is evaluated through the content of the six ingredients, that is to say, if the total sum of the content of the six ingredients is larger than 10%, the Brazilian green propolis is high-quality propolis, and if the total sum of the content of the six ingredients is smaller than 6%, the Brazilian green propolis is low-quality propolis. According to the method, the chemical ingredients of the Brazilian green propolis are combined with the antioxidant activity, the antioxidant material basis of the Brazilian green propolis is illustrated, the defect that some main ingredients do not achieve the high pharmacological activity is avoided, and the antioxidant activity of the Brazilian green propolis can be reflected more truly; the design is reasonable, the irrationality that in an existing method, total flavonoid is taken as the quality control index of the Brazilian green propolis is overcome, the defect that content detection of multiple flavones or phenolic acids has no correlation with the activity is overcome, and a novel method is provided for Brazilian green propolis quality control.