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Application of WAP (whey acidic protein) gene in transgenosis of fruit fly

A transgenic, fruit fly technology, applied in application, genetic engineering, plant genetic improvement and other directions, can solve the problems of incalculable social significance and economic value of antimicrobial peptide drugs, low yield and activity, insoluble recombinant protein, etc. Antibacterial function and viability, significant antibacterial effect, high expression effect

Active Publication Date: 2014-06-18
山东辰泰药业有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the social significance and economic value of antimicrobial peptide drugs in saving lives and safeguarding human health are inestimable
[0005] Although antimicrobial peptides show great potential for new drug development, there are still many problems in the production of most antimicrobial peptides as drugs, such as no protease inhibitory activity and easy to be hydrolyzed by proteases, the content in the organism is too low to directly separate and purify from biological tissues, prokaryotic The expressed recombinant protein is insoluble, the yield and activity are not high, etc.
At present, there are very few antimicrobial peptide drugs that have been successfully developed.

Method used

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  • Application of WAP (whey acidic protein) gene in transgenosis of fruit fly
  • Application of WAP (whey acidic protein) gene in transgenosis of fruit fly
  • Application of WAP (whey acidic protein) gene in transgenosis of fruit fly

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Embodiment Construction

[0043] The present invention will be further described below.

[0044] (1) Construction of WAP transgenic fruit flies: According to the sequence at both ends of the ORF of the antimicrobial peptide gene WAP containing a single whey acidic protein domain in Penaeus chinensis, a pair of upstream and downstream primers Wap-f and Wap-r were designed, and the upstream and downstream The 5' ends of the primers were introduced separately kpn I(GGTACC) with xba I (AGATCA) restriction site, the primer sequence is as follows:

[0045] Wap-f: 5'---GG GGTACC ATGGTGAACATCAAGGAAGTTC---3' (SEQ ID NO: 1)

[0046] Wap-r: 5'---GCTCT AGATCA T TTTCCGTAGGGAGATCCCA ---3' (SEQ ID NO: 2)

[0047] Using the cDNA of hemocytes of Penaeus chinensis as a template, the WAP fragment was caught by PCR, and the kpn I and xba ⅠTwo restriction sites were directionally connected with the pUAST plasmid to construct the eukaryotic expression vector wap / pUAST, and the sequence of the inserted WAP fra...

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Abstract

The invention relates to an application of a WAP (whey acidic protein) gene in transgenosis of a fruit fly. According to the application, the WAP gene in Fenneropenaeus chinensis is constructed in the fruit fly and is expressed in the fruit flies. The transgenic fruit fly prepared by utilizing the application has an antibacterial effect.

Description

technical field [0001] The invention relates to the application of a WAP gene in fruit fly transgenesis. Background technique [0002] With the widespread use or even abuse of antibiotics, the problems of antibiotic residues and bacterial resistance have attracted more and more attention from all over the world, and the research and development of new antibacterial drugs to replace traditional antibiotics has also become a worldwide research topic. [0003] Antimicrobial peptides are small molecular polypeptides with strong antibacterial effects produced in organisms. They have a very important defensive effect against the invasion of pathogens such as bacteria, viruses, and fungi. They are considered to be new types of anti-infective drugs that are difficult to cause microbial resistance. It has good application prospects in the treatment and prevention of cancer, anti-virus and anti-infection, and has become a hot spot in the development of new antibacterial drugs (Splith ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12C12N15/85A01K67/033A61K35/64A61P31/04
Inventor 李殿香王金星赵小凡王蕾齐梅刘力王军王显伟黄健黄加栋李强刘晖王国荣赵艳霞李宁矫强胡杨车彤彤周雷叶春江
Owner 山东辰泰药业有限公司
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