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Molecular marker of TrkA gene as sheep lambing number characteristic and application of molecular marker

A molecular marker, sheep technology, applied in application, genetic engineering, plant genetic improvement, etc., can solve the problems of long breeding cycle, low selection accuracy, and inability to carry out early breeding.

Active Publication Date: 2016-09-21
甘肃羊如祥农业有限公司
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Problems solved by technology

[0005] The purpose of the present invention is to overcome the defects of the prior art, especially the technical defects such as the long breeding cycle of the existing sheep, the low selection accuracy, and the inability to carry out early breeding, and provide a molecular marker for improving the correlation of sheep litter size traits as an auxiliary means of choice

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  • Molecular marker of TrkA gene as sheep lambing number characteristic and application of molecular marker
  • Molecular marker of TrkA gene as sheep lambing number characteristic and application of molecular marker
  • Molecular marker of TrkA gene as sheep lambing number characteristic and application of molecular marker

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Embodiment Construction

[0027] The following examples are used to further illustrate the present invention, but should not be construed as limiting the present invention. On the premise of not departing from the spirit and essence of the present invention, all modifications or replacements made to the present invention belong to the protection scope of the present invention.

[0028] 1. Blood sample collection

[0029] Peripheral blood samples were collected from sheep with first-born lambing records (breeds Hu sheep and Small-tailed Han sheep), and the blood samples were collected from Jinchang Zhongtian Sheep Industry Co., Ltd., Gansu Province.

[0030] 2. Genomic DNA extraction

[0031] Genomic DNA in the collected sheep blood samples was extracted by traditional DNA extraction method (ie phenol / chloroform method). Specific steps are as follows:

[0032] (1) Take 1 mL of collected sheep whole blood and add an equal volume of PBS buffer, and shake gently for 10 min;

[0033] (2) After centrifug...

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Abstract

The invention belongs to the technical field of sheep molecular marker screening and application and relates to a molecular marker of TrkA gene as the sheep lambing number characteristic and application of the molecular marker. The molecular marker is cloned from the TrkA gene (Gene Bank ID: 101109763). The nucleotide sequence of the molecular marker is shown as SEQ ID NO:1, and C> exists in the positon of the 432 bit basic group of the shown sequence SEQ ID NO.1; T base substitution mutates, and the mutation causes SacII-RFLP enzyme digestion polymorphism. Related correlation analysis application is performed on Hu sheep and small-tailed Han sheep. A novel genetic marker resource is provided for marker assisted selection of the sheep lambing number characteristic.

Description

technical field [0001] The invention belongs to the technical field of sheep molecular marker screening, and in particular relates to TrkA gene as a molecular marker for lambing traits of sheep and its application. The molecular marker of the present invention is cloned from sheep TrkA gene. Background technique [0002] In traditional breeding methods, the selection of livestock traits mostly depends on the phenotype of livestock. This method requires extensive experience and decades of time in large livestock breeding. For some quantitative traits with low heritability controlled by minor polygenes, such as sheep lambing traits, which are not only determined by the ewe's own genetic factors, but also affected by different environments and feeding conditions, conventional breeding methods have made slow progress in genetic improvement. Although morphological markers, cytological markers, biochemical markers and immunological markers have been applied in the breeding of fin...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/54C12N15/11C12Q1/68
Inventor 李万宏翁秀秀乐祥鹏张军霞王维民李发弟
Owner 甘肃羊如祥农业有限公司
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