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Brevibacillus brevis for preventing and treating plant fungus diseases and method for preparing biopesticide

A technology for Bacillus brevis, plant fungal diseases, applied in botanical equipment and methods, microorganism-based methods, biochemical equipment and methods, etc. Effective control effect

Inactive Publication Date: 2011-08-17
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prevention and control of crop wilt, due to the stubbornness, diversity and variability of pathogenic bacteria, there is still a lack of effective chemical agents and effective disease-resistant varieties, which has become a key problem that needs to be solved urgently in the production of melons and other crops

Method used

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  • Brevibacillus brevis for preventing and treating plant fungus diseases and method for preparing biopesticide
  • Brevibacillus brevis for preventing and treating plant fungus diseases and method for preparing biopesticide
  • Brevibacillus brevis for preventing and treating plant fungus diseases and method for preparing biopesticide

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Experimental program
Comparison scheme
Effect test

Embodiment

[0038] The screening method of Bacillus brevis for preventing and treating plant fungal diseases comprises the following steps:

[0039] (1) Take soil samples: Select a well-growing watermelon field, shovel off 2-3cm of topsoil in the rhizosphere of healthy watermelon plants, and take 10g of soil near the watermelon root; put it into a sterilized kraft paper bag, Seal the bag well, and record the sampling location, environment and date.

[0040] (2) Isolation and purification of bacterial strains: isolate and purify the bacterial strains in the retrieved soil samples by gradient dilution plate streaking method, the specific operation is as follows:

[0041] Heat and melt the prepared beef extract peptone solid medium (NA medium) to prepare a plate; weigh 1g of soil sample, add 10ml of sterile water, add sterilized glass beads and shake for 15 minutes, determine the concentration to be 1, and let stand Finally, take 1.0 mL of the supernatant and add it to a test tube filled ...

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Abstract

The invention relates to brevibacillus brevis for preventing and treating plant fungous diseases and application thereof. The brevibacillus brevis has a gene sequencing result of 1367bp, has an accession number of HQ585418 on a gene bank, is preserved in the China general microbiological culture collection center (CGMCC) and has a preservation number of CGMCC NO.4361. A method for preparing a brevibacillus brevis biopesticide for preventing and treating the plant fungous diseases comprises the following operation steps of: 1, preparing seed liquid; and 2, fermenting and preparing the brevibacillus brevis biopesticide. A field experiment proves that the brevibacillus brevis can obviously reduce the morbidity of cucurbit wilt, and thus, a strain disclosed by the invention can be effectively applied to prevention and treatment of cucurbit wilt and can be used for preparing the safe and high-efficiency biopesticide.

Description

technical field [0001] The invention belongs to the technical field of biological pesticides, and in particular relates to bacillus brevis for preventing and treating fungal diseases of plants and a preparation method of biological pesticides thereof. Background technique [0002] The long-term use of chemical pesticides has caused serious consequences: the pesticide residues in agricultural products and soil are getting higher and higher, which seriously reduces the quality of agricultural products; the density of beneficial organisms has decreased, and the natural community has been severely damaged; pathogenic bacteria and pests are resistant to pesticides. The general increase has led to the excessive use of chemical pesticides, increased the production cost of agricultural products, and aggravated the damage to the environment, forming a vicious circle. With the enhancement of people's awareness of environmental protection, biopesticides are highly valued by various cou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N15/31A01N63/02A01P3/00C12R1/08
Inventor 胡雪芹芮广虎张洪斌苏晓飞
Owner HEFEI UNIV OF TECH
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