Inkpad for anti-counterfeiting by using single nucleotide polymorphous biogenetic marker
A technology of genetic markers and single nucleotides, applied in the measurement/inspection of microorganisms, biochemical equipment and methods, applications, etc., can solve the problems of easy imitation, difficulty in distinguishing authenticity, loss of anti-counterfeiting effect, etc., to prevent deciphering and forgery , long-lasting effect
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[0021] The present invention will be further described below in conjunction with specific examples.
[0022] (1) Select the oral swab as the sample, and use the product of Tiangen Biochemical Technology Co., Ltd. (rapid DNA extraction and amplification kit) to obtain genomic DNA;
[0023] (2), select the VEGF, P53, P21 gene fragments containing SNP sites in the genomic DNA as the target gene fragments, use existing public database resources to query and screen SNP sites with higher occurrence rates, and use primer premier 5.0 , BLAST primer and GeneBank software, query and design the sequence of the upstream primer and downstream primer corresponding to the target gene fragment, the design direction of the primer is 5'-3'; the details are as follows:
[0024] (2.1) VEGF 287bp
[0025] 5’-TCGGAAGCCGGGCTCATGGACGGGTGAGGCGGCGGTGTGCGCAGACAGTGCTCCAGCCGCGCGCGCTCCCCAGGCCCTGGCCCGGGCCTCGGGCCGGGGAGGAAGAGTAGCTCGCCGAGGCGCCGAGGAGAGCGGGCCGCCCCACAGCCCGAGCCGGAGAGGGAGCGCGAGCCGCGCCGGCCCCGGTCGGG...
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