Inkpad for anti-counterfeiting by using single nucleotide polymorphous biogenetic marker

A technology of genetic markers and single nucleotides, applied in the measurement/inspection of microorganisms, biochemical equipment and methods, applications, etc., can solve the problems of easy imitation, difficulty in distinguishing authenticity, loss of anti-counterfeiting effect, etc., to prevent deciphering and forgery , long-lasting effect

Inactive Publication Date: 2010-11-24
叶欣
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the traditional technology is mature and widely used, it has the disadvantages of open technology, easily available formula principle, very easy to imitate, and difficult to distinguish the authenticity from the fake.
Traditional anti-counterfeiting technology has gradually lost its anti-counterfeiting effect and urgently needs to be upgraded
At the same time, traditional anti-counterfeiting technology cannot achieve personal information anti-counterfeiting
Emerging technologies such as photopolymer holographic marks and ultra-thin security plastic sealing films have good anti-counterfeiting effects, but the production costs are high and the application fields are narrow

Method used

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Examples

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Effect test

Embodiment Construction

[0021] The present invention will be further described below in conjunction with specific examples.

[0022] (1) Select the oral swab as the sample, and use the product of Tiangen Biochemical Technology Co., Ltd. (rapid DNA extraction and amplification kit) to obtain genomic DNA;

[0023] (2), select the VEGF, P53, P21 gene fragments containing SNP sites in the genomic DNA as the target gene fragments, use existing public database resources to query and screen SNP sites with higher occurrence rates, and use primer premier 5.0 , BLAST primer and GeneBank software, query and design the sequence of the upstream primer and downstream primer corresponding to the target gene fragment, the design direction of the primer is 5'-3'; the details are as follows:

[0024] (2.1) VEGF 287bp

[0025] 5’-TCGGAAGCCGGGCTCATGGACGGGTGAGGCGGCGGTGTGCGCAGACAGTGCTCCAGCCGCGCGCGCTCCCCAGGCCCTGGCCCGGGCCTCGGGCCGGGGAGGAAGAGTAGCTCGCCGAGGCGCCGAGGAGAGCGGGCCGCCCCACAGCCCGAGCCGGAGAGGGAGCGCGAGCCGCGCCGGCCCCGGTCGGG...

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PUM

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Abstract

The invention relates to an inkpad for anti-counterfeiting by using a single nucleotide polymorphous biogenetic marker. The inkpad comprises a common inkpad and is characterized by further comprising 0.1 to 10 weight percent of target gene segment PCR amplification product mixture and 0.001 to 0.1 weight percent of solution of a DNA enzyme inhibitor, wherein the target gene segment PCR amplification product is obtained by amplifying a SNP locus-containing gene segment. The inkpad amplifies part of segments from a complicated genome DNA by searching a sequence where the conventional known SNP locus is in a gene bank and by designing a gene segment of a specificity amplification individual of a primer and effectively prevents other people from decoding and counterfeiting. At the same time, the DNA is stable and can be stored for a long time, even for thousands of years.

Description

technical field [0001] The invention belongs to the technical field of biological anti-counterfeiting, and in particular relates to an anti-counterfeiting ink pad using single nucleotide polymorphism (SNP) biological genetic markers. Background technique [0002] In recent years, due to the increasingly serious counterfeiting activities and the need for prevention, the use of anti-counterfeiting technology has attracted more and more attention from companies in various countries. Many conventional anti-counterfeiting technologies such as laser anti-counterfeiting, ink and paper anti-counterfeiting, and electronic code anti-counterfeiting have been widely used. Although the traditional technology is mature and widely used, it has the disadvantages of open technology, easily available formula principle, very easy to imitate, and difficult to distinguish the authenticity. Traditional anti-counterfeiting technology has gradually lost its anti-counterfeiting effect and urgently ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09D11/02C12Q1/68G01N21/64
Inventor 叶欣
Owner 叶欣
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