Method for separating corn bacterial wilt pythium

A technology of Pythium wilt and solanacear, applied in the field of separation, can solve the problems of imperfect separation of corn bacterial wilt pathogen and no separation method, etc., and achieve the effects of being convenient for wide application, improving labor efficiency, and reducing production costs

Inactive Publication Date: 2012-07-04
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the isolation of the pathogenic bacteria of corn bacterial wilt is not perfect, and there is no feasible isolation method

Method used

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  • Method for separating corn bacterial wilt pythium
  • Method for separating corn bacterial wilt pythium
  • Method for separating corn bacterial wilt pythium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] A method utilizing separation medium to isolate the pathogenic Pythium solanacearum of corn, comprising the following steps:

[0067] Step 1, the preparation of antibiotic culture medium

[0068] 1) Add sterile deionized water to penicillin sodium with a concentration of 800,000 U / mL, mix well, and prepare a mother solution a with a concentration of 200,000 U / mL for later use;

[0069] 2) Add sterile deionized water to streptomycin sulfate with a concentration of 1 million U / bottle, mix well, and prepare mother solution b with a concentration of 200,000 U / mL, and set aside;

[0070] 3) Take 1 part of mother solution a with a concentration of 200,000 U / mL and 1 part of mother solution b with a concentration of 200,000 U / mL according to the ratio of parts by weight, and mix them evenly to prepare a compound with a concentration of 100,000 U / mL Antibiotic mother solution, spare;

[0071] Step 4) Add 400g of potatoes into 4000mL of water, cook for 30 minutes, filter to ob...

Embodiment 2

[0096] A method utilizing separation medium to isolate the pathogenic Pythium solanacearum of corn, comprising the following steps:

[0097] Step 1, the preparation of antibiotic culture medium

[0098] 1) Add sterile deionized water to penicillin sodium with a concentration of 800,000 U / mL, mix well, and prepare a mother solution a with a concentration of 200,000 U / mL for later use;

[0099] 2) Add sterile deionized water to streptomycin sulfate with a concentration of 1 million U / bottle, mix well, and prepare mother solution b with a concentration of 200,000 U / mL, and set aside;

[0100] 3) Take 1 part of mother solution a with a concentration of 200,000 U / mL and 1 part of mother solution b with a concentration of 200,000 U / mL according to the ratio of parts by weight, and mix them evenly to prepare a compound with a concentration of 100,000 U / mL Antibiotic mother solution, spare;

[0101] Step 4) Add 500g of potatoes into 4000mL of water, boil for 35 minutes, filter to ob...

Embodiment 3

[0126] A method utilizing separation medium to isolate the pathogenic Pythium solanacearum of corn, comprising the following steps:

[0127] Step 1, the preparation of antibiotic culture medium

[0128] 1) Add sterile deionized water to penicillin sodium with a concentration of 800,000 U / mL, mix well, and prepare a mother solution a with a concentration of 200,000 U / mL for later use;

[0129] 2) Add sterile deionized water to streptomycin sulfate with a concentration of 1 million U / bottle, mix well, and prepare mother solution b with a concentration of 200,000 U / mL, and set aside;

[0130] 3) Take 1 part of mother solution a with a concentration of 200,000 U / mL and 1 part of mother solution b with a concentration of 200,000 U / mL according to the ratio of parts by weight, and mix them evenly to prepare a compound with a concentration of 100,000 U / mL Antibiotic mother solution, spare;

[0131] Step 4) Add 600g of potatoes into 4000mL of water, cook for 40 minutes, filter to ob...

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Abstract

The invention relates to a method for separating corn bacterial wilt pythium and in particular relates to separation of corn bacterial wilt pythium by utilizing a separation culture medium. The method comprises the following steps of: designing and synthesizing primers, extracting DNA (Deoxyribonucleic Acid) of corn bacterial wilt pythium, amplifying and separating a target gene, separating the amplified final product, sequencing and identifying, and comparing in a gene bank to determine to be corn bacterial wilt pythium. According to the method for separating corn bacterial wilt pythium by utilizing the separation culture medium provided by the invention, the identification method is simple to operate, the identification result is rapid and accurate, and the method can be applied to practical production.

Description

technical field [0001] The invention relates to a separation method, in particular to a method for separation of corn bacterial wilt pathogen Pythium. Background technique [0002] Maize bacterial wilt is a soil-borne disease that commonly occurs in maize producing areas in the world. It also occurs successively in corn producing areas all over my country and is relatively serious, thus seriously affecting the yield of corn. Generally, the incidence rate of corn bacterial wilt is 10%-20%, and the incidence is serious in rainy seasons, up to more than 80%. Under normal circumstances, according to the speed of disease development and field symptoms, it can be divided into acute type and common type. The acute type mostly occurs in the late stage of corn growth when it rains and then clears up. It is also called the green withered type. The vascular bundles can be seen at the base have turned brown, and finally lead to water-soaked necrosis, and the ears droop. The common ty...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/02C12R1/645
Inventor 侯军林晓民陈跟强胡梅孙亚莉
Owner HENAN UNIV OF SCI & TECH
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