Method for separating corn bacterial wilt pythium
A technology of Pythium wilt and solanacear, applied in the field of separation, can solve the problems of imperfect separation of corn bacterial wilt pathogen and no separation method, etc., and achieve the effects of being convenient for wide application, improving labor efficiency, and reducing production costs
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Embodiment 1
[0066] A method utilizing separation medium to isolate the pathogenic Pythium solanacearum of corn, comprising the following steps:
[0067] Step 1, the preparation of antibiotic culture medium
[0068] 1) Add sterile deionized water to penicillin sodium with a concentration of 800,000 U / mL, mix well, and prepare a mother solution a with a concentration of 200,000 U / mL for later use;
[0069] 2) Add sterile deionized water to streptomycin sulfate with a concentration of 1 million U / bottle, mix well, and prepare mother solution b with a concentration of 200,000 U / mL, and set aside;
[0070] 3) Take 1 part of mother solution a with a concentration of 200,000 U / mL and 1 part of mother solution b with a concentration of 200,000 U / mL according to the ratio of parts by weight, and mix them evenly to prepare a compound with a concentration of 100,000 U / mL Antibiotic mother solution, spare;
[0071] Step 4) Add 400g of potatoes into 4000mL of water, cook for 30 minutes, filter to ob...
Embodiment 2
[0096] A method utilizing separation medium to isolate the pathogenic Pythium solanacearum of corn, comprising the following steps:
[0097] Step 1, the preparation of antibiotic culture medium
[0098] 1) Add sterile deionized water to penicillin sodium with a concentration of 800,000 U / mL, mix well, and prepare a mother solution a with a concentration of 200,000 U / mL for later use;
[0099] 2) Add sterile deionized water to streptomycin sulfate with a concentration of 1 million U / bottle, mix well, and prepare mother solution b with a concentration of 200,000 U / mL, and set aside;
[0100] 3) Take 1 part of mother solution a with a concentration of 200,000 U / mL and 1 part of mother solution b with a concentration of 200,000 U / mL according to the ratio of parts by weight, and mix them evenly to prepare a compound with a concentration of 100,000 U / mL Antibiotic mother solution, spare;
[0101] Step 4) Add 500g of potatoes into 4000mL of water, boil for 35 minutes, filter to ob...
Embodiment 3
[0126] A method utilizing separation medium to isolate the pathogenic Pythium solanacearum of corn, comprising the following steps:
[0127] Step 1, the preparation of antibiotic culture medium
[0128] 1) Add sterile deionized water to penicillin sodium with a concentration of 800,000 U / mL, mix well, and prepare a mother solution a with a concentration of 200,000 U / mL for later use;
[0129] 2) Add sterile deionized water to streptomycin sulfate with a concentration of 1 million U / bottle, mix well, and prepare mother solution b with a concentration of 200,000 U / mL, and set aside;
[0130] 3) Take 1 part of mother solution a with a concentration of 200,000 U / mL and 1 part of mother solution b with a concentration of 200,000 U / mL according to the ratio of parts by weight, and mix them evenly to prepare a compound with a concentration of 100,000 U / mL Antibiotic mother solution, spare;
[0131] Step 4) Add 600g of potatoes into 4000mL of water, cook for 40 minutes, filter to ob...
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