Redroot wild broad bean tissue culture rapid propagation new method

A technology of tissue culture rapid propagation and wild broad bean, which is applied in the field of tissue culture rapid propagation of red root wild broad bean, can solve the problems of long germination cycle, over-harvesting of wild resources, low germination rate, etc., and achieve low cost, fast reproduction speed, The effect of high reproductive rate

Active Publication Date: 2018-11-20
INST OF MEDICINAL PLANTS YUNNAN ACAD OF AGRI SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

By 2014, the price of its red-rooted wild broad bean has been increasing rapidly due to the shortage of medicinal materials in the market, while wild resources are propagated by seeds, which have a long germination cycle and a very low germination rate, resulting in excessive harvesting of wild resources

Method used

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  • Redroot wild broad bean tissue culture rapid propagation new method
  • Redroot wild broad bean tissue culture rapid propagation new method
  • Redroot wild broad bean tissue culture rapid propagation new method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1. Select healthy red-rooted wild broad bean plants as explants, cut off the stem end 10-20cm, cut into about 2-3cm in size with buds (axillary buds or terminal buds) and rinse with saturated soapy water After 15 minutes, rinse with tap water 3-4 times until no soap remains, add appropriate amount of water, add 2-3 drops of Tween-80, shake for 10 minutes, and rinse with tap water for 60 minutes.

[0019] 2. Transfer the cleaned explants to a sterile bottle in the ultra-clean workbench, add 75% alcohol to shake for 15 seconds, rinse with sterile water twice, then add saturated bleach solution and shake for 15 minutes, then shake with sterile water Rinse twice, and finally add 0.05% mercuric acid solution and 1 drop of Tween-80, shake for 8 minutes, and rinse with sterile water 6 times.

[0020] 3. Absorb the moisture on the surface of the explants with sterile paper, cut off the wounds at both ends to a 0.5-1.0cm size bud stem section, and insert it obliquely in 1 / 2WPM+N...

Embodiment 2

[0024] 1. Select healthy red-rooted wild broad bean plants as explants, cut off the stem end 10-20cm, cut into about 2-3cm in size with buds (axillary buds or terminal buds) and rinse with saturated soapy water After 15 minutes, rinse with tap water 3-4 times until no soap remains, add appropriate amount of water, add 2-3 drops of Tween-80, shake for 10 minutes, and rinse with tap water for 60 minutes.

[0025] 2. Transfer the cleaned explants to a sterile bottle in the ultra-clean workbench, add 75% alcohol to shake for 15 seconds, rinse with sterile water twice, then add saturated bleach solution and shake for 15 minutes, then shake with sterile water Rinse twice, and finally add 0.05% mercuric acid solution and 1 drop of Tween-80, shake for 8 minutes, and rinse with sterile water 6 times.

[0026] 3. Absorb the water on the surface of the explant with sterile paper, cut off the wound at both ends to a 0.5-1.0cm size bud stem section, and insert it obliquely in 1 / 4MS+NAA 0.0...

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Abstract

The invention discloses a redroot wild broad bean tissue culture rapid propagation new method. The redroot wild broad bean tissue culture rapid propagation new method comprises the steps of selectingexplants, cleaning and disinfecting the explants, inducing multiple shoots, proliferating multiple shoots, rooting and the like. A tissue culture technology of redroot wild broad beans is explored; the ideal culture conditions of obtaining a sterile material, inducing the multiple shoots, proliferating the multiple shoots, rooting the multiple shoots and the like are determined; a tissue culture system of the redroot wild broad beans is successfully established finally; technical basis is laid for realizing protection and cultivation of redroot wild broad bean wild resources, demand on the seedlings and establishment of a gene bank; the problems of overdevelopment of the redroot wild broad bean wild resources, little medicine supply and scaleless planting are solved.

Description

technical field [0001] The invention relates to a method for tissue culture and rapid propagation of Chinese medicinal materials, which belongs to the field of plant tissue culture and planting of Chinese medicinal materials, in particular to a method for tissue culture and rapid propagation of red-root wild broad bean. Background technique [0002] Red-root wild broad bean (Centranthera grandiflora Benth) is a herbaceous plant of the family Scrophulariaceae. The whole herb is used as medicine. It has the effect of dispelling blood stasis and stopping bleeding; reducing swelling and pain. It is mainly used for hemoptysis; coughing up blood; vomiting blood; women's amenorrhea; blood; rheumatic arthralgia in middle-aged and elderly people; muscle and bone pain; cerebral thrombosis; cerebral hemorrhage; alcoholic liver; gastroenteritis; pharyngitis; oral ulcers; bruises; gynecological inflammation; gastric cancer; It is mainly distributed in Yunnan, Guizhou, Guangxi and other p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张金渝赵露琴左应梅曾祥飞
Owner INST OF MEDICINAL PLANTS YUNNAN ACAD OF AGRI SCI
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