A kind of spoon-leaf sundew tissue culture propagation method

A technology of sundew and tissue culture, which is applied in the field of tissue culture and propagation of sundew spoon-leaf, can solve the problems of difficult management and maintenance of insect-catching characteristics of sundew spoon-leaf, and achieve fast propagation speed, long collection period, and rooting high rate effect

Active Publication Date: 2021-05-11
云南昊辰农业有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In recent years, spoon-leaf sundew has been widely recognized by the market due to its interesting insect-catching characteristics and easy management and maintenance. The market demand has increased sharply, but limited to traditional propagation techniques, the demand for seedlings has become a bottleneck for its market expansion.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Plant strong plants indoors, spray 2 times with 75% carbendazim 500 times solution every 4 days, and before the inflorescences are drawn out to bloom, select the explants at the end of the inflorescence axis 8-12 cm;

[0019] (2) Dip the selected explants with a soft-bristled toothbrush to wash the surface of the diluted detergent, then rinse with tap water until there is no detergent residue, then rinse under tap water for 50 minutes, and transfer to the ultra-clean workbench , use sterile cotton dipped in 75% alcohol to wipe the surface of the explant 2 to 3 times, rinse with sterile water 2 to 3 times after each wipe, and then cut it into 1.2cm pieces, first disinfect it with 3% hydrogen peroxide for 15 minutes , rinse with sterile water 2 to 3 times, then disinfect with 0.05% mercury solution for 4 minutes, and finally rinse with sterile water for 7 to 9 times.

[0020] (3) Absorb the surface moisture of the sterilized explants with sterile filter paper, cut off...

Embodiment 2

[0024] (1) Plant healthy plants indoors, spray 3 times with 75% carbendazim 500 times solution every 5 days, and before the inflorescences are drawn out and bloomed, select the explants at the end of the inflorescence axis 8-12 cm;

[0025] (2) Dip the selected explants with a soft-bristled toothbrush to wash the surface of the diluted detergent, then rinse with tap water until there is no detergent residue, then rinse under tap water for 60 minutes, and transfer to the ultra-clean workbench , use sterile cotton dipped in 75% alcohol to wipe the surface of the explant 2 to 3 times, rinse with sterile water 2 to 3 times after each wipe, and then cut it into 1.5cm pieces, first disinfect it with 3% hydrogen peroxide for 20 minutes , rinse with sterile water 2 to 3 times, then disinfect with 0.05% mercuric solution for 5 minutes, and finally rinse with sterile water for 7 to 9 times.

[0026] (3) Absorb the surface moisture of the sterilized explants with sterile filter paper, cu...

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PUM

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Abstract

The invention discloses a method for tissue culture and propagation of sundew spoon leaf, which comprises (1) explant selection, (2) explant disinfection, (3) cluster bud induction, (4) cluster bud proliferation, (5) A series of steps for clustering shoots and rooting. The tissue culture propagation method of the invention significantly improves the induction rate, multiplication coefficient and rooting rate of the sundew spoon-leaf sundew, has high tissue culture efficiency, low cost, and has market application value.

Description

technical field [0001] The invention relates to the field of flower tissue culture, in particular to a method for tissue culture and propagation of sundew spoon leaf. Background technique [0002] Spoon-leaf sundew (Drosera spathulata Labill.Var. spathulata) is a herbaceous plant of the Drosera family Drosera genus. It has the habit of preying on insects and is a highly ornamental and interesting flower plant. It is mainly distributed in Yunnan, western Guizhou, southwestern Sichuan, southern Tibet and other places in my country. It is also distributed in India, Japan, Malaysia, Laos and other countries. It often grows in pine forests, sparse forests, grasses and shrubs at an altitude of 1200m to 3650 meters. Edges, watersides, and lawns are also visible. Now it is cultivated in most parts of the country. Spoon-leaf sundew is now cultivated as a flower plant. Leaf cuttings and division propagation are the main production methods of spoon-leaf sundew at present, but its repr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 邓清张金渝曾琼柴丹杨天梅杨维泽赵文芹杨绍兵李纪潮余正强
Owner 云南昊辰农业有限公司
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