A kind of curculigo leaf in vitro culture and plant regeneration method

Abstract

The invention discloses a method for in vitro cultivation and regeneration of curculigo grass leaves, comprising the steps of selecting explants, cleaning and disinfecting the explants, inducing clustered buds, proliferating clustered buds, and rooting. The present invention successfully establishes a low-cost tissue culture and rapid propagation system of curculigo, which lays a technical foundation for realizing the protection, artificial cultivation, seedling cultivation and establishment of gene banks of curculigo wild resources, and solves the problem of over-exploitation of curculigo wild resources, The supply of medicinal materials is insufficient, the planting is not in scale, the planting is limited by the supply of seedlings, and the quality of medicinal materials is unstable.

Description

technical field [0001] The invention relates to a method for tissue culture and rapid propagation of Chinese medicinal materials, and belongs to the field of plant tissue culture and planting of Chinese medicinal materials, in particular to a method for in vitro cultivation and plant regeneration of curculigo grass leaves. Background technique [0002] Curculigo orchioides Gaertn. is a herbaceous plant of the genus Curculigo orchioides in the family Amaryllidaceae. It is used as medicine with rhizomes. It has the effects of nourishing kidney yang, strengthening muscles and bones, and dispelling cold and dampness. Cold pain, yang deficiency and cold diarrhea embolism. [0003] Curculigo curculigo is mainly distributed in Yunnan, Guizhou, Sichuan, Guangxi, Guangdong, Hunan, Fujian, Taiwan, Jiangxi, Zhejiang and other places, and grows in forests, grasslands or barren slopes below an altitude of 1600m. Also distributed in Southeast Asian countries to Japan. [0004] Curculigo...

AI Technical Summary

Problems solved by technology

At the same time, the limited wild resources not only cannot meet the market demand, Curculigo may become endangered resources

Some individuals and units have gradually begun to carry out artificial planting experiments of curculigo, and artificially carried out rhizome cut bud propagation and seed propagation research, and found that the reproduction rate was low during rhizome cut bud propagation, the root rot was serious, and the seed propagation It is difficult to harvest seeds (the seeds are on the surface of the stem base), the amount of seeds is small, and the germination rate of seeds is low. It is difficult to use traditional propagation methods for large-scale and standardized artificial planting.

In recent years, domestic and foreign enterprises and institutions have carried out research on the tissue culture technology of curculigo, but they all use underground rhizomes as explants for propagation, which has the disadvantages of high pollution rate and low induction rate. The situation of planting is serious, and it needs to be reinoculated in the rooting medium after expansion, and there are disadvantages such as weak growth of seedlings and relatively high production costs.