A kind of curculigo leaf in vitro culture and plant regeneration method

A technology of in vitro culture and leaves, applied in plant regeneration, botany equipment and methods, horticultural methods, etc., can solve problems such as difficult scale, standardized artificial planting, curculigo resources are on the verge of extinction, and cannot meet market demand, etc., to achieve The effect of reducing tissue culture cost, convenient preparation, and fast reproduction speed

Active Publication Date: 2022-01-25
INST OF MEDICINAL PLANTS YUNNAN ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, the limited wild resources not only cannot meet the market demand, Curculigo may become endangered resources
Some individuals and units have gradually begun to carry out artificial planting experiments of curculigo, and artificially carried out rhizome cut bud propagation and seed propagation research, and found that the reproduction rate was low during rhizome cut bud propagation, the root rot was serious, and the seed propagation It is difficult to harvest seeds (the seeds are on the surface of the stem base), the amount of seeds is small, and the germination rate of seeds is low. It is difficult to use traditional propagation methods for large-scale and standardized artificial planting.
In recent years, domestic and foreign enterprises and institutions have carried out research on the tissue culture technology of curculigo, but they all use underground rhizomes as explants for propagation, which has the disadvantages of high pollution rate and low induction rate. The situation of planting is serious, and it needs to be reinoculated in the rooting medium after expansion, and there are disadvantages such as weak growth of seedlings and relatively high production costs.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1. Select healthy and free of diseases and insect pests as explants, rinse with saturated soapy water solution for 10 minutes, rinse with tap water 3 times until no soap remains, add appropriate amount of water, add 3 drops of Tween-80, shake 10 minutes, rinse with tap water for 50 minutes;

[0019] 2. Transfer the cleaned explants to a sterile bottle in the ultra-clean workbench, add 75% alcohol and shake for 10 seconds, rinse with sterile water for 3 times, then add saturated bleach solution and shake for 20 minutes, then shake with sterile water Rinse 3 times, finally add 0.05% mercuric chloride solution and shake for 10 minutes, rinse 6 times with sterile water;

[0020] 3. Absorb the moisture on the surface of the explants with sterile paper, cut off the disinfectant wound, cut it into 0.5cm pieces, and spread it on MS+2,4-D 0.1mg / L+6-BA 1.0mg On the induction medium of / L+activated carbon 0.5g / L, the culture temperature is 25°C, and the culture light is dark cult...

Embodiment 2

[0023] 1. Choose healthy and free from diseases and insect pests as explants, rinse with saturated soapy water solution for 15 minutes, rinse with tap water 3 times until no soap remains, add appropriate amount of water, add 4 drops of Tween-80, shake 15 minutes, rinse with tap water for 60 minutes;

[0024] 2. Transfer the cleaned explants to a sterile bottle in the ultra-clean workbench, add 75% alcohol to shake for 15 seconds, rinse with sterile water for 4 times, then add saturated bleach solution and shake for 30 minutes, then shake with sterile water Rinse 4 times, finally add 0.05% mercuric acid solution and shake for 15 minutes, rinse with sterile water 8 times;

[0025] 3. Absorb the moisture on the surface of the explants with sterile paper, cut off the disinfectant wound, cut it into 1cm pieces, spread it on MS+2,4-D 0.5mg / L+6-BA 3.0mg / On the induction medium of L+activated carbon 0.5g / L, the culture temperature is 25°C, and the culture light is dark culture for t...

Embodiment 3

[0028] 1. Choose healthy curculigo root-like stems without pests and diseases as explants, wash them with tap water 5 times, brush off the surface soil with a toothbrush, and scrape off the surface of the depression with a scalpel, then rinse with saturated soapy water for 15 minutes, and rinse with tap water for 3 minutes. Once there is no soap liquid residue, add appropriate amount of water, add 3 drops of Tween-80, shake for 10 minutes, and rinse with tap water for 50 minutes;

[0029] 2. Transfer the cleaned explants to a sterile bottle in an ultra-clean workbench, add 75% alcohol and shake for 30 seconds, rinse with sterile water for 3 times, then add saturated bleach solution and shake for 40 minutes, then shake with sterile water Rinse 3 times, finally add 0.1% mercuric solution + 3 drops of Tween-80, shake for 10 minutes, rinse 6 times with sterile water;

[0030] 3. Absorb the moisture on the surface of the explants with sterile paper, cut off the disinfectant wound, ...

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PUM

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Abstract

The invention discloses a method for in vitro cultivation and regeneration of curculigo grass leaves, comprising the steps of selecting explants, cleaning and disinfecting the explants, inducing clustered buds, proliferating clustered buds, and rooting. The present invention successfully establishes a low-cost tissue culture and rapid propagation system of curculigo, which lays a technical foundation for realizing the protection, artificial cultivation, seedling cultivation and establishment of gene banks of curculigo wild resources, and solves the problem of over-exploitation of curculigo wild resources, The supply of medicinal materials is insufficient, the planting is not in scale, the planting is limited by the supply of seedlings, and the quality of medicinal materials is unstable.

Description

technical field [0001] The invention relates to a method for tissue culture and rapid propagation of Chinese medicinal materials, and belongs to the field of plant tissue culture and planting of Chinese medicinal materials, in particular to a method for in vitro cultivation and plant regeneration of curculigo grass leaves. Background technique [0002] Curculigo orchioides Gaertn. is a herbaceous plant of the genus Curculigo orchioides in the family Amaryllidaceae. It is used as medicine with rhizomes. It has the effects of nourishing kidney yang, strengthening muscles and bones, and dispelling cold and dampness. Cold pain, yang deficiency and cold diarrhea embolism. [0003] Curculigo curculigo is mainly distributed in Yunnan, Guizhou, Sichuan, Guangxi, Guangdong, Hunan, Fujian, Taiwan, Jiangxi, Zhejiang and other places, and grows in forests, grasslands or barren slopes below an altitude of 1600m. Also distributed in Southeast Asian countries to Japan. [0004] Curculigo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张金渝邓清杨绍兵曾祥飞杨维泽左应梅沈晓明杨天梅曾芸
Owner INST OF MEDICINAL PLANTS YUNNAN ACAD OF AGRI SCI
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