A kind of Tinospora tissue culture rapid propagation method

A technology for tissue culture rapid propagation and green ox bile is applied in horticultural methods, botanical equipment and methods, plant regeneration, etc. The effect of industrial promotion value, fast reproduction speed and high tissue culture efficiency

Active Publication Date: 2021-08-27
INST OF MEDICINAL PLANTS YUNNAN ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The medicinal herb Lavender, which uses Tinobilis as the plant-based source, is a cold-backed medicinal material. However, in recent years, with the research, development and application of uses, the demand for Lavender has increased by leaps and bounds, and the limited wild resources cannot meet the market demand. , its price has also risen rapidly thereupon, and various enterprises and institutions have carried out planting technology research on green bile gall, but due to the small amount of green cattle gallbladder seeds, low seed germination rate, and long germination cycle, the traditional seed reproduction rate is low, It cannot meet the needs of large-scale planting. Although some enterprises and institutions have made breakthroughs in cutting and layering propagation, they all have the dilemma of extremely low reproduction rate and slow growth.
In recent years, some units have started the research on the tissue culture technology of A. chinensis and achieved success, but there is no report on the research on the tissue culture and rapid propagation technology of Tinospora.

Method used

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  • A kind of Tinospora tissue culture rapid propagation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Plant strong plants indoors, spray 3 times with 500 times of 75% carbendazim every 7 days, and select young tender stem segments of 20-30 cm at the end as explants;

[0022] (2) Remove the leaves from the selected explants, cut them into 2-3 cm bud stem segments, wash them with saturated soapy water, then use a soft toothbrush to remove the dirt from the epidermis and depressions, and then rinse them with tap water for 3-4 days. Add 2-3 drops of Tween-80 and appropriate amount of water until no soap remains, shake for 15 minutes, and rinse with tap water for 60 minutes. Transfer to an ultra-clean workbench, disinfect with 75% alcohol for 40 seconds, rinse with sterile water for 2 to 3 times, disinfect with saturated bleach solution for 40 minutes, rinse with sterile water for 2 to 3 times, and rinse with 0.1% mercury solution + 2 to 3 drops. Disinfect at -80 for 15 minutes, rinse with sterile water 6 times;

[0023] (3) Use sterile paper to absorb the surface moist...

Embodiment 2

[0028] (1) Plant strong plants indoors, spray 4 times with 500 times of 75% carbendazim every 7 days, and select the young and tender stem segments of 20-30 cm at the end as explants;

[0029] (2) Remove the leaves from the selected explants, cut them into 2-3 cm bud stem segments, wash them with saturated soapy water, then use a soft toothbrush to remove the dirt from the epidermis and depressions, and then rinse them with tap water for 3-4 days. Add 2-3 drops of Tween-80 and appropriate amount of water until no soap remains, shake for 10 minutes, and rinse with tap water for 50 minutes. Transfer to an ultra-clean workbench, disinfect with 75% alcohol for 30 seconds, rinse with sterile water for 2 to 3 times, disinfect with saturated bleach solution for 30 minutes, rinse with sterile water for 2 to 3 times, and rinse with 0.1% mercury solution + 2 to 3 drops. Disinfect at -80 for 10 minutes, rinse with sterile water 7 times;

[0030] (3) Use sterile paper to absorb the surfa...

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Abstract

The invention discloses a method for rapid propagation by tissue culture of ox gall bladder, comprising (1) explant selection, (2) explant disinfection, (3) cluster bud induction, (4) cluster bud proliferation, and (5) cluster bud proliferation Rooting series of steps. The invention establishes a high-efficiency and low-cost tissue culture and rapid propagation system of ox bile, which makes the large-scale cultivation of ox bile possible.

Description

technical field [0001] The invention relates to the technical field of tissue culture of traditional Chinese medicinal materials, in particular to a tissue culture and rapid propagation method of Tinospora biloba. Background technique [0002] Tinospora sagittata (Oliv.) Gagnep. is a plant of the genus Tinospora sagittata (Oliv.) Gagnep. Its roots are used as medicine. Carbuncle cures poison, has loose bowels, dysentery, epigastric pain. Mainly distributed in Hubei, Sichuan, Tibet, Guizhou, Yunnan, Hunan, Jiangxi, Fujian, Guangxi. [0003] The medicinal herb Lavender, which uses Tinobilis as the plant-based source, is a cold-backed medicinal material. However, in recent years, with the research, development and application of uses, the demand for Lavender has increased by leaps and bounds, and the limited wild resources cannot meet the market demand. , its price has also risen rapidly thereupon, and various enterprises and institutions have carried out planting technology ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张金渝蒋元省杨维泽曾祥飞杨美权许宗亮赵露琴
Owner INST OF MEDICINAL PLANTS YUNNAN ACAD OF AGRI SCI
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