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A kind of tissue culture method of Uncaria

A technology of tissue culture and Uncaria, applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve the problems that the planting scale and production capacity cannot meet the market demand, the quality of medicinal materials is uneven, and the price of Uncaria medicinal materials has risen. , achieve the effect of fast reproduction speed, easy operation, and keeping the parental traits

Active Publication Date: 2021-08-27
INST OF MEDICINAL PLANTS YUNNAN ACAD OF AGRI SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In recent years, with the increase of its development use and the improvement of clinical recognition of its curative effect, the demand has been increasing year by year, and the price of Uncaria medicinal materials has also risen accordingly. The current planting scale production capacity can no longer meet the market demand
Traditional propagation methods mainly include seed propagation and cutting propagation. Seed propagation has disadvantages such as long propagation cycle and unstable germplasm traits, while cutting propagation has disadvantages such as low reproduction rate and serious pests and diseases during seedling and planting process.
At the same time, most of Uncaria's medicinal material standards have detection indicators for rhynchophylline content in various companies. Due to the large number of Uncaria species, even the differences between different varieties of the same species are large, resulting in uneven quality of medicinal materials.

Method used

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  • A kind of tissue culture method of Uncaria
  • A kind of tissue culture method of Uncaria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1. Selection of explants: Select the robust and pest-free branches of Uncaria in the current year, cut 10-20cm from the stem end as explants, and cut them into stem segments with buds (axillary buds or terminal buds) of about 2-3cm in size. Rinse with saturated soapy water solution for 10min, then rinse with tap water 3-4 times until no soap residue remains, add appropriate amount of water, add 3 drops of Tween-80, shake for 10min, and rinse with tap water for 60min;

[0020] 2. Disinfection: pour out all the running water, transfer the cleaned explants to the ultra-clean workbench, add 75% alcohol and shake for 30s, rinse with sterile water for 3 times, and then add saturated bleaching powder solution and shake for 20min. Rinse with sterile water for 3 times, finally add 0.1% mercuric chloride solution + 1 drop of Tween-80 and shake for 6 minutes, rinse with sterile water for 6 times;

[0021] 3. Induction of clump buds: the sterilized explants were sucked off the surf...

Embodiment 2

[0025] 1. Selection of explants: Select the robust and pest-free branches of Uncaria in the current year, cut 10-20cm from the stem end as explants, and cut them into stem segments with buds (axillary buds or terminal buds) of about 2-3cm in size. Rinse with saturated soapy water solution for 10min, then rinse with tap water for 3-4 times until no soap residue remains, add appropriate amount of water, add 2-3 drops of Tween-80, shake for 10min, and rinse with tap water for 60min;

[0026] 2. Disinfection: pour out all the running water, transfer the cleaned explants to the ultra-clean workbench, add 75% alcohol and shake for 26s, rinse with sterile water for 3 times, and then add saturated bleaching powder solution and shake for 25min. Rinse with sterile water for 3 times, finally add 0.1% mercuric chloride solution + 1 drop of Tween-80 and shake for 8 minutes, rinse with sterile water for 6 times;

[0027] 3. Induction of clump buds: The sterilized explants were soaked with s...

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Abstract

The invention discloses a tissue culture method of Uncaria uncaria, which comprises the steps of selecting explants, cleaning and disinfecting the explants, inducing clustered buds, proliferating clustered buds, rooting and the like. The present invention explores Uncaria tissue culture technology, establishes ideal culture conditions such as the acquisition of sterile materials, the induction of clustered buds, the proliferation of clustered buds, and the rooting of clustered buds, and finally successfully establishes the tissue culture system of Uncaria. It lays a technical foundation for the protection of wild Uncaria wild resources, the preservation of high-quality germplasm, and the large-scale planting of high-quality germplasm, and solves the problems of over-exploitation of wild Uncaria wild resources and standardized and large-scale planting of seedlings.

Description

technical field [0001] The invention relates to a tissue culture method of traditional Chinese medicinal materials, belonging to the field of plant tissue culture and planting of traditional Chinese medicinal materials, in particular to a tissue culture method of Uncaria chinensis. Background technique [0002] Uncaria rhynchophylla (Miq.) Miq.ex Havil. is a perennial woody vine of the Rubiaceae family. Uncaria rhynchophylla (Miq.) Wind movement, epilepsy convulsions, febrile convulsions, children screaming, pregnancy eclampsia, headache and dizziness. Modern medicine studies that it contains rhynchophylline, which has the effects of lowering blood pressure, anti-platelet aggregation and anti-thrombosis, and is used to treat some cardiovascular and cerebrovascular diseases. At present, there are about 170 kinds of traditional Chinese medicine preparations using Uncaria as the raw material, and the annual demand for medicinal materials is large. The wild is mainly distribut...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张金渝邓清杨绍兵柴丹杨维泽尹进超杨天梅蒋元省杨美权熊毅
Owner INST OF MEDICINAL PLANTS YUNNAN ACAD OF AGRI SCI
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