A new method for tissue culture and rapid propagation of red root wild broad bean

A technology of tissue culture rapid propagation and wild broad bean, which is applied in the field of tissue culture rapid propagation of red root wild broad bean, can solve the problems of excessive harvesting of wild resources, low germination rate, long germination cycle, etc., and achieve fast reproduction speed and high reproduction rate , low cost effect

Active Publication Date: 2021-08-27
INST OF MEDICINAL PLANTS YUNNAN ACAD OF AGRI SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

By 2014, the price of its red-rooted wild broad bean has been increasing rapidly due to the shortage of medicinal materials in the market, while wild resources are propagated by seeds, which have a long germination cycle and a very low germination rate, resulting in excessive harvesting of wild resources

Method used

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  • A new method for tissue culture and rapid propagation of red root wild broad bean

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Effect test

Embodiment 1

[0017] 1. Select healthy red-rooted wild broad bean plants as explants, cut off the stem end 10-20cm, cut into about 2-3cm in size with buds (axillary buds or terminal buds) and rinse with saturated soapy water After 15 minutes, rinse with tap water 3-4 times until no soap remains, add appropriate amount of water, add 2-3 drops of Tween-80, shake for 10 minutes, and rinse with tap water for 60 minutes.

[0018] 2. Transfer the cleaned explants to a sterile bottle in the ultra-clean workbench, add 75% alcohol to shake for 15 seconds, rinse with sterile water twice, then add saturated bleach solution and shake for 15 minutes, then shake with sterile water Rinse twice, and finally add 0.05% mercuric acid solution and 1 drop of Tween-80, shake for 8 minutes, and rinse with sterile water 6 times.

[0019] 3. Absorb the water on the surface of the explants with sterile paper, cut off the wounds at both ends to a 0.5-1.0cm size with bud stems, and insert them obliquely in 1 / 2WPM+NAA ...

Embodiment 2

[0023] 1. Select healthy red-rooted wild broad bean plants as explants, cut off the stem end 10-20cm, cut into about 2-3cm in size with buds (axillary buds or terminal buds) and rinse with saturated soapy water After 15 minutes, rinse with tap water 3-4 times until no soap remains, add appropriate amount of water, add 2-3 drops of Tween-80, shake for 10 minutes, and rinse with tap water for 60 minutes.

[0024] 2. Transfer the cleaned explants to a sterile bottle in the ultra-clean workbench, add 75% alcohol to shake for 15 seconds, rinse with sterile water twice, then add saturated bleach solution and shake for 15 minutes, then shake with sterile water Rinse twice, and finally add 0.05% mercuric acid solution and 1 drop of Tween-80, shake for 8 minutes, and rinse with sterile water 6 times.

[0025] 3. Absorb the water on the surface of the explant with sterile paper, cut off the wound at both ends to a 0.5-1.0cm size bud stem section, and insert it obliquely in 1 / 4MS+NAA 0.0...

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Abstract

The invention discloses a tissue culture fast propagation method of red-root wild broad bean, which includes the steps of selection of explants, cleaning and disinfection of explants, induction of clump buds, proliferation of clump buds and rooting. The invention explores the tissue culture and rapid propagation technology of red root wild bean, establishes ideal culture conditions such as the acquisition of sterile materials, the induction of clump buds, the proliferation of clump buds, the rooting of clump buds, etc., and finally successfully established the red root The tissue culture and rapid propagation system of wild broad bean has laid a technical foundation for the protection, cultivation, demand for seedlings and the establishment of gene bank of wild red root wild bean, and solved the over-exploitation of wild red root wild bean wild resources and medicinal materials. Problems such as low supply and insufficient planting.

Description

technical field [0001] The invention relates to a method for tissue culture and rapid propagation of Chinese medicinal materials, which belongs to the field of plant tissue culture and planting of Chinese medicinal materials, in particular to a method for tissue culture and rapid propagation of red-root wild broad bean. Background technique [0002] Red-root wild broad bean (Centranthera grandiflora Benth) is a herbaceous plant of the family Scrophulariaceae. The whole herb is used as medicine. It has the effect of dispelling blood stasis and stopping bleeding; reducing swelling and pain. It is mainly used for hemoptysis; coughing up blood; vomiting blood; women's amenorrhea; blood; rheumatic arthralgia in middle-aged and elderly people; muscle and bone pain; cerebral thrombosis; cerebral hemorrhage; alcoholic liver; gastroenteritis; pharyngitis; oral ulcers; bruises; gynecological inflammation; gastric cancer; It is mainly distributed in Yunnan, Guizhou, Guangxi and other p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张金渝赵露琴左应梅曾祥飞
Owner INST OF MEDICINAL PLANTS YUNNAN ACAD OF AGRI SCI
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